Strain Information
| Name | GLW37 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | lin-5(utx29[mNG::3xFlag::lin-5]) II. |
| Description | N-terminal tag of LIN-5 via CRISPR/Cas9 knock-in of mNeonGreen at lin-5 locus. Insertion verified by PCR and fluorescence. Left flank: 5' ttttctttgaataattactccttctgcaga 3'; Right flank: 5' ATGAGCGTGAGCACATCAGTTGTGAAGTCA 3'; sgRNA: GCTCACGCTCATtctgcaga; Cas9/sgRNA plasmid: pGLOW32; mNG^SEC^3xFlag plasmid: pGLOW33; SEC insertion allele strain (balanced): GLW36. |
| Mutagen | CRISPR/Cas9 homologous recombination |
| Outcrossed | x0 |
| Made by | Alex Koh (Glow Worms '20) |
| Laboratory | GLW |
| Reference | N/A |
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