Strain Information
| Name | GLW35 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | gldi-2(utx27[mNG::3xFlag::T13C2.6]) II. |
| Description | T13C2.6. N-terminal tag of T13C2.6 via CRISPR/Cas9 knock-in of mNeonGreen at gldi-2 locus. Insertion verified by PCR and fluorescence. Left flank: 5' aaatattaattgataatcagaggagtaaaa 3'; Right flank: 5' ATGAGGACATGTCTCACCCTCACGGGTTTC 3'; sgRNA: taatcagaggagtaaaaATG; Cas9/sgRNA plasmid: pGLOW45; mNG^SEC^3xFlag plasmid: pGLOW54; SEC insertion allele strain: GLW34. |
| Mutagen | CRISPR/Cas9 homologous recombination |
| Outcrossed | x0 |
| Made by | Staci Guillen (Glow Worms '21) |
| Laboratory | GLW |
| Reference | N/A |
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