Strain Information

Name GLW2   View On Wormbase
Species C. elegans
Genotypeattf-2(utx2[mNG::attf-2]) V.
DescriptionSuperficially wild-type. N-terminal tag of ATTF-2 via CRISPR/Cas9 knock-in of mNeonGreen at attf-2 locus. Insertion verified by PCR. Left flank: 5' cttttttgctcacatcatcatttttcagtc 3'; Right flank: 5' ATGTCGGCCGAGACCGCGACTATCCCCGAAGTTTC 3' (5 silent mutations). sgRNA: 5' CGAAACTGCAACCATACCCG 3'
MutagenCRISPR/Cas9 homologous recombination
Made byGeorge Huang (Glow Worms '20)
Laboratory GLW
Reference N/A
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