Strain Information
| Name | ERC102 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | ieSi57 II; smc-3(syb5520[smc-3::GGGGS::AID::emGFP]) III. |
| Description | ieSi57 [eft-3p::TIR1::mRuby::unc-54 3'UTR + Cbr-unc-119(+)] II. Single copy transgene inserted into chromosome II (oxTi179) expressing modified Arabidopsis thaliana TIR1 tagged with mRuby in the soma. Degron and emGFP tag inserted into endogenous smc-3 locus. Derived by crossing parental strains PHX5520 with CA1200. Reference: https://www.biorxiv.org/content/10.1101/2023.09.18.558239v1. |
| Mutagen | No mutagen |
| Outcrossed | x1 |
| Made by | Jun Kim |
| Laboratory | ERC |
| Reference | https://www.biorxiv.org/content/10.1101/2023.09.18.558239v1 The degron-GFP allele was generated by SUNY Biotech PHX5520 smc-3(syb5520) sequence: GATTTTGTTGAGGATGACACGACTCATGGAGGGGGAGGAGGATCGATGCCTAAAGATCCAGCCAAACCTCCGGCCAAGGCACAAGTTGTGGGATGGCCACCGGTGAGATCATACCGGAAGAACGTGATGGTTTCCTGCCAAAAATCAAGCGGTGGCCCGGAGGCGGCGGCGTTCGTGAAGGTTTCTAAAGGAGAAGAACTTTTCACAGGAGTTGTTCCAATACTTGTTGAACTTGATGGAGATGTAAATGGACATAAGTTCTCTGTTTCTGGCGAAGGAGAAGGAGATGCTACTTATGGAAAACTTACTCTCAAGTTCATTTGCACTACTGGAAAACTTCCCGTTCCATGGCCAACTCTTGTTACTACTTTgACTTATGGAGTTCAATGTTTCGCTCGTTATCCAGACCATATGAAACAACACGATTTCTTCAAATCTGCTATGCCAGAAGGATATGTTCAAGAAAGAACTATTTTCTTCAAAGATGATGGAAACTACAAAACTCGTGCGGAAGTAAAGTTTGAGGGAGATACTCTTGTAAATAGAATAGAACTCAAAGGAATAGATTTCAAAGAAGATGGAAATATACTTGGACATAAACTTGAATATAACTACAATAGTCATAAAGTTTATATTACTGCTGATAAACAAAAAAATGGAATAAAAGTAAACTTCAAAACTCGTCATAATATAGAAGATGGAAGTGTTCAACTTGCTGACCATTACCAACAAAATACTCCAATAGGAGATGGCCCAGTTCTTCTCCCAGATAATCATTATCTTTCTACTCAATCTGCTCTTTCTAAAGACCCAAATGAAAAAAGAGACCATATGGTTCTTTTAGAGTTCGTTACTGCGGCGGGAATAACTCTTGGAATGGATGAACTTTACAAATAAatatatgcatattttgaatattttttt |
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