Strain Information
| Name | EAG16 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | eagIs6[*fxIs10] II. |
| Description | eagIs6 [spn-4p::jGCaMP7s::pie-1 3'UTR + HygR [*fxIs10] ] II. CaFE reporter (calcium inducible fluorescence in germline). Calcium-inducible fluorescent jGCaMP7s protein codon-optimized for elegans and expressed in germline enables visualization of calcium wave upon fertilization. Reference: Toperzer KM, et al. Biol Open. 2023 Sep 15;12(9):bio059832. PMID: 37602653. |
| Mutagen | No mutagen |
| Outcrossed | x0 |
| Made by | Karen Kim Guisbert |
| Laboratory | EAG |
| Reference | Visualization of the biphasic calcium wave during fertilization in Caenorhabditis elegans using a genetically encoded calcium indicator. Toperzer et al. Biol. Open (2023) 12 (9): bio059832. https://doi.org/10.1242/bio.059832 |
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