Strain Information

Name DQM543   View On Wormbase
Species C. elegans
GenotypebmdSi147 I.
DescriptionbmdSi147 [loxN::rps-27p::DHB::2xmKate2::P2A::H2B::GFP] I. bmdSi147 is a single-copy CRISPR/Cas9-engineered insertion of a codon-optimized CDK sensor (amino acids 994–1087 of human DNA Helicase B (DHB) fused to two copies of mKate2) co-expressed with his-58 (H2B) fused to GFP. Reference: Adikes RC, et al. "Visualizing the metazoan proliferation-terminal differentiation decision in vivo." bioRxiv 2019.12.18.881888
MutagenCrispr/Cas9
Outcrossedx0
Made byTaylor N. Medwig-Kinney
Laboratory DQM
Reference "Visualizing the metazoan proliferation-terminal differentiation decision in vivo Rebecca C. Adikes, Abraham Q. Kohrman, Michael A. Q. Martinez, Nicholas J. Palmisano, Jayson J. Smith, Taylor N. Medwig-Kinney, Mingwei Min, Maria D. Sallee, Ononnah B. Ahmed, Nuri Kim, Simeiyun Liu, Robert D. Morabito, Nicholas Weeks, Qinyun Zhao, Wan Zhang, Jessica L. Feldman, Michalis Barkoulas, Ariel M. Pani, Sabrina L. Spencer, Benjamin L. Martin, David Q. Matus bioRxiv 2019.12.18.881888; doi: https://doi.or
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