Strain Information
| Name | DQM1256 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | bmdSi346 I; bmdSi297 II. |
| Description | bmdSi346 [loxN::lin-31p::FLP]; inserted into safe harbor site ttTi4348 in Chr I. bmdSi297[loxN::rpl-28p::FRT3::STOP::FRT3::TIR1(F79G)::T2A::DHB::2xmK ate2]; inserted into safe harbor site ttTi5605 in Chr II. FLP-ON::TIR1 system for AID-tagged protein degradation in VPCs. High levels of TIR1(F79G) expression in vulval precursor cells by lin-31p::FLP with co-expression of CDK activity sensor. bmdSi297 contains the ubiquitous rpl-28 promoter driving expression of FRT3::STOP::FRT3::TIR1(F79G)::DHB construct dependent upon tissue-specific FLPase. High levels of TIR1(F79G) can be expressed in specific tissue or cell types via FLPase activity, allowing spatiotemporally-targeted degradation of AID-tagged proteins. Reference: Xiao Y, et al. An expandable FLP-ON::TIR1 system for precise spatiotemporal protein degradation in C. elegans. bioRxiv 2022.10.14.512315; doi: https://doi.org/10.1101/2022.10.14.512315 |
| Mutagen | No mutagen |
| Outcrossed | x2 |
| Made by | Matus Lab |
| Laboratory | DQM |
| Reference | An expandable FLP-ON::TIR1 system for precise spatiotemporal protein degradation in C. elegans Yutong Xiao, View ORCID ProfileCallista Yee, Michael A.Q. Martinez, Chris Z Zhao, Wan Zhang, Kang Shen, View ORCID ProfileDavid Q Matus, View ORCID ProfileChristopher M. Hammell doi: https://doi.org/10.1101/2022.10.14.512315 |
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