Strain Information

Name DQM1138   View On Wormbase
Species C. elegans
Genotypedpff-1(bmd302[dpff-1p::^SEC^mNG::AID::DPFF-1]) III.
DescriptionPick Rollers to maintain. Endogenous N-term tagged DPFF-1 with mNG::AID using the self-excising cassette for drug selection. Animals are rollers which contains sqt-1 gene. Remove the SEC for normal expression using the protocol described in "Dickinson DJ, Pani AM, Heppert JK, Higgins CD, Goldstein B. Streamlined Genome Engineering with a Self-Excising Drug Selection Cassette. Genetics. 2015 Aug;200(4):1035-49. doi: 10.1534/genetics.115.178335. Epub 2015 Jun 3. PMID: 26044593; PMCID: PMC4574250."
Made byYutong Xiao
Laboratory DQM
Reference We are going to make a micropub about this allele, describing it facilitates visualization of the endogenous DPFF-1 protein expression in vivo, and allow people to achieve conditional depletion.
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