Strain Information

Name DAG355   View On Wormbase
Species C. elegans
Genotypelite‐1(ce314) X; domIs355.
DescriptiondomIs355 [mec‐3p::QF + mec‐4p::QS + QUAS::CoChR::GFP + unc122p::RFP]. High sensitivity blue-light optogenetic line for FLP neurons. Transgenic animals expressing the high-sensitivity blue light-activated channelrhodopsin CoChR into FLP using the Q-system combining mec-3p and mec-4p promoters. In animals grown on all trans-retinal-containing medium, low intensity blue light stimuli trigger reversal responses. Animals have red coelomocytes. The transgene was integrated with UV, and outcrossed 2x to parental ce314 mutant strain KG1180. Reference: Schild LC & Glauser DA. Genetics. 2015 Aug;200(4):1029-34. doi: 10.1534/genetics.115.177956. PMID: 26022242.
Made byLisa Schild
Laboratory DAG
Reference Schild LC, Glauser DA. Dual Color Neural Activation and Behavior Control with Chrimson and CoChR in Caenorhabditis elegans. Genetics. 2015 Aug;200(4):1029-34. doi: 10.1534/genetics.115.177956. Epub 2015 May 28. PMID: 26022242; PMCID: PMC4574232.
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