Strain Information
| Name | DAG253 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | lite‐1(ce314) X; domEx253. |
| Description | domEx253 [mec‐4p::Chrimson::GFP + unc‐122p::RFP]. Pick animals with red fluorescence in coelomocytes to maintain. Red-light optogenetic line for gentle touch receptor neurons (TRN). Transgenic animals expressing the red light-activated channelrhodopsin Chrimson into TRNs using the mec-4 promoter. In animals grown on all trans-retinal-containing medium, red light stimuli trigger behaviors similar to those evoked by gentle touch. Note that very strong blue light stimuli may also activate Chrimson. Reference: Schild LC & Glauser DA. Genetics. 2015 Aug;200(4):1029-34. doi: 10.1534/genetics.115.177956. PMID: 26022242. |
| Mutagen | No mutagen |
| Outcrossed | x0 |
| Made by | Lisa Schild |
| Laboratory | DAG |
| Reference | Schild LC, Glauser DA. Dual Color Neural Activation and Behavior Control with Chrimson and CoChR in Caenorhabditis elegans. Genetics. 2015 Aug;200(4):1029-34. doi: 10.1534/genetics.115.177956. Epub 2015 May 28. PMID: 26022242; PMCID: PMC4574232. |
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