Strain Information

Name CZ24324   View On Wormbase
Species C. elegans
GenotypemuIs32 II; qns-1(ju1563)/mIs11 IV.
DescriptionmuIs32 [mec-7p::GFP + lin-15(+)]. mIs11 [myo-2p::GFP + pes-10p::GFP + F22B7.9::GFP]. GFP expression in 4-cell embryos, pharyngeal muscle and gut. Heterozygotes are WT with dim GFP signal in the pharynx. mIs11 homozygotes are WT with bright GFP in the pharynx. qns-1(ju1563) animals are sterile. Pick WT with dim GFP+ in pharynx to maintain. mIs11 homozygotes will quickly overtake the population if not selected against. Reference: Kim KW, et al. Elife. 2018 Nov 21;7:e39756. doi: 10.7554/eLife.39756. PMID: 30461420
MutagenCrispr/Cas9
Outcrossedx2
Made byKyung Won Kim
Laboratory 4112
Reference Expanded genetic screening in Caenorhabditis elegans identifies new regulators and an inhibitory role for NAD+ in axon regeneration. Kim KW, Tang NH, Piggott CA, Andrusiak MG, Park S, Zhu M, Kurup N, Cherra SJ 3rd, Wu Z, Chisholm AD, Jin Y. Elife. 2018 Nov 21;7:e39756. doi: 10.7554/eLife.39756.
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