Strain Information
| Name | CGC98 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | lin-4(umn10[lin-4p::mScarlet-I + Lox511I::let-858 3'UTR sqt-1(d) hsp::CRE hygR Lox511I::let-858 3'UTR])/mIn1[dpy-10(e128) umnIs33] II. |
| Description | umnIs33 [myo-2p::GFP + NeoR, II: 11755713 (intergenic)] II. lin-4 deletion allele in which lin-4 pre-miRNA (II, lin4:5902254..5902347) was replaced by mScarlet. Heterozygotes are GFP+ mScarlet+ Rollers, and segregate GFP+ mScarlet+ Rollers, non-GFP mScarlet+ lin-4 homozygotes, and Dpy GFP+ mIn1 homozygotes. Maintain by picking wild-type GFP+ mScarlet+ and check for correct segregation of progeny to maintain. Generated in parental strain N2. [NOTE: Low levels of Cre activity can lead to excision of the SEC, causing the strain to lose the Roll phentoype. Pick Rollers to retain full transgene cassette.] |
| Mutagen | |
| Outcrossed | x0 |
| Made by | Julie Knott |
| Laboratory | CGC |
| Reference | n/a |
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