Strain Information
| Name | CGC181 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | umnDf1[mir-35p+SL1::EGL13NLS::lox2272] II. |
| Description | Deletion of mir-35, mir-36, mir-37, mir-38, mir-39 and mir-40 miRNA. A cassette containing mScarlet-I and an SEC flanked by lox2272 was introduced into the mir-35 loci using CRISPR/Cas9. This line was generated by excising mScarlet-I and the SEC leaving a SL1::EGL13NLS::lox2272 scar. Left Flanking: CCTCTCCTAATTTCCATTCCCAACTATTAT, Right Flanking: GCTCTTTTTTTGCTGTTTTTAAACTTAAAC. sgRNA #1: ACTGTGGAAGAAACCAGCAA. sgRNA #2: GGTGAAAAATCACCTAGGTC. |
| Mutagen | Crispr/Cas9 |
| Outcrossed | x0 |
| Made by | Julie Knott and Marcus L. Vargas |
| Laboratory | CGC |
| Reference | n/a |
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