Strain Information
| Name | CGC176 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | lin-4(umn83[lin-4p::SL1::EGL-13NLS::lox2272::::mScarlet-I::cMycNLS:: lox511I sqt-1(d) hsp::CRE HygR Lox511I::let-858 3'UTR::lox2722])/mIn1[dpy-10(e128) umnIs33] II. |
| Description | umnIs33 [myo-2p::GFP + NeoR, II: 11755713 (intergenic)] II. Weak-nuclear mScarlet-I was inserted in place of the endogenous lin-4 pre-miRNA via CRISPR/CAS9. Heterozygotes are Rol+, mScarlet+ GFP+, and segregate Rol+ mScarlet+ GFP+, Lin-4 Rol mScarlet+ non-GFP (umn83 homozygotes), and Dpy non-mScarlet GFP+ (mIn1 homozygotes). Maintain by picking wild-type mScarlet+ GFP+. Left Flanking: AGAGTTTTGGTTGGTTTATGAGTTT, Right Flanking: CCAGGACGGTTTGAGCAGATCtttt. sgRNA: TGAGGTCTCAGGGAACAGGC. |
| Mutagen | Crispr/Cas9 |
| Outcrossed | x0 |
| Made by | Marcus L. Vargas |
| Laboratory | CGC |
| Reference | n/a |
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