Strain Information
Name | CGC153 View On Wormbase |
---|---|
Species | C. elegans |
Genotype | mir-48(umn60[mir-48p+SL1::EGL-13NLS::mScarlet-I::cMycNLS::linker::mODC(422-461)(E428A/E430A/E431A):: lox511I::let-858 3'UTR]) V. |
Description | Nuclear mScarlet-I was inserted in place of the endogenous mir-48 pre-miRNA via CRISPR/CAS9. Left Flanking: CACAGGTAAGTCAATTAACCAATTG, Right Flanking: TTATTATTATGTTTCATTCAATAAC. sgRNA: GGGAATGCGAGCTAGGCTGG. |
Mutagen | Crispr/Cas9 |
Outcrossed | x0 |
Made by | Julie Knott & Marcus Vargas |
Laboratory | CGC |
Reference | n/a |
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