Strain Information
| Name | CGC118 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | lin-4(umn29[lin-4p+SL1long+Kozak::egl-13-NLS::mScarlet-I::c-myc-NLS + Lox511I sqt-1(d) hsp::CRE hygR Lox511I])/mIn1[dpy-10(e128) umnIs33] II. |
| Description | umnIs33 [myo-2p::GFP + NeoR, II: 11755713 (intergenic)] II. lin-4 pre-miRNA deletion strain deletion allele in which lin-4 pre-miRNA was replaced by myo-2p::wrmScarlet; includes SL1(long) and Kozak sequence upstream of mScarlet. Heterozygotes are Rol GFP+ mScarlet+, and segregate GFP+ mScarlet+ heterozygotes, non-GFP mScarlet+ lin-4 homozygotes, and Dpy GFP+ mIn1 homozygotes. Maintain by picking Rol GFP+ mScarlet+ and check for correct segregation of progeny to maintain. Derived by CRE-meditated excision of SEC in parental strain CGC118, leaving lin-4p::mScarlet. |
| Mutagen | Crispr/Cas9 |
| Outcrossed | x0 |
| Made by | Julie Knott & Marcus Vargas |
| Laboratory | CGC |
| Reference | n/a |
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