Strain Information
| Name | CFJ302 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | unc-119(kst33) III. |
| Description | Maintain at 15C. Temperature-sensitive unc-119 allele. Wild-type at 15C, intermediate Unc and Egl at 20C, and fully penetrant Unc and Egl at 25C. For use in transgenesis, maintain the strain at lower temperatures for increased brood size and easier injection, then transfer animals to 25C to select for transgenic animals based on Unc rescue. Molecular characterization shows a complex allele with a 210 bp duplication from a nearby exon-intron junction, which introduces 12 amino acids and a putative splice donor at a consensus splice acceptor site. The phenotype is most likely caused by temperature-sensitive splicing defects based on RT-PCR. Reference: Aljohani M, et al. Arrayed oligo libraries: genome-wide DNA- and RNP-based platforms for templated and non-templated CRISPR-Cas9 editing in C. elegans. (Submitted) |
| Mutagen | Crispr/Cas9 |
| Outcrossed | x0 |
| Made by | Mohammed Aljohani |
| Laboratory | CFJ |
| Reference | Aljohani M, Vargas-Velazquez A, Frøkjær-Jensen C. Arrayed oligo libraries: genome-wide DNA- and RNP-based platforms for templated and non-templated CRISPR-Cas9 editing in C. elegans. (Submitted) |
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