Strain Information

Name BV70   View On Wormbase
Species C. elegans
Genotypeunc-119(ed3) III; zbIs2.
DescriptionzbIs2 [pie-1p::lifeact::RFP::pie-1 3' UTR + unc-119(+)]. Lifeact tag labels F-actin without interfering F-actin dynmics. The strain has never been officially published; however, a GFP version of this transgene is described in Pohl C & Bao Z. Dev Cell. 2010 Sep 14;19(3):402-12. PMID: 20833362.
Made byZhirong Bao
Laboratory BV
Reference The strain has never been officially published. However, the GFP version of the strain is published here: Christian Pohl, Zhirong Bao (2010) Chiral Forces Organize Left-Right Patterning in C. elegans by Uncoupling Midline and Anteroposterior Axis, Developmental Cell,Volume 19, Issue 3, 402-412. The following recent publications from other labs make use of the strain directly or a modified transgenic strain based on our strain: Priti, A., Ong, H.T., Toyama, Y. et al. Syncytial germline architecture is actively maintained by contraction of an internal actomyosin corset. Nat Commun 9, 4694 (2018). Fazeli G, Trinkwalder M, Irmisch L, Wehman AM. C. elegans midbodies are released, phagocytosed and undergo LC3-dependent degradation independent of macroautophagy. J Cell Sci. 2016 Oct 15;129(20):3721-3731. doi: 10.1242/jcs.190223. Epub 2016 Aug 25. PMID: 27562069; PMCID: PMC5087666. Hu J, Cheng S, Wang H, Li X, Liu S, et al. (2019) Distinct roles of two myosins in C. elegans spermatid differentiation. PLOS Biology 17(4): e3000211. Padmanabhan A, Ong HT, Zaidel-Bar R. Non-junctional E-Cadherin Clusters Regulate the Actomyosin Cortex in the C. elegans Zygote. Curr Biol. 2017 Jan 9;27(1):103-112. doi: 10.1016/j.cub.2016.10.032. Epub 2016 Dec 15. PMID: 27989674.
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