Strain Information
| Name | BRC546 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | antIs30 II; unc-119(ed9) III. |
| Description | antIs30 [attP-f + Cbr-unc-119(+) + glh-2p::phiC31 + rol-6(partial) + myo-2p::GFP + attP-r] II. antIs30 was inserted into ttTi5605 on LG II using MosSCI. GFP expression in pharynx is very weak (as it is single copy) and is easiest to see during the L1-L3 stages. This strain contains a phiC31 docking site and can be used for precise single-copy integration of transgenes via recombination mediated cassette exchange. The docking site contains inverted phiC31-attP sites flanking phiC31 integrase expressed from the glh-2 germline promoter. Integration constructs need to have inverted phiC31-attB sites that flank the intended sequence to be inserted. Reference: Yang FJ, et al. "phiC31 integrase for recombination mediated single copy insertion and genome manipulation in C. elegans." Genetics 2021. |
| Mutagen | MosSCI |
| Outcrossed | x0 |
| Made by | Fang-Jung Yang |
| Laboratory | BRC |
| Reference | Manuscript in revision at Genetics phiC31 integrase for recombination mediated single copy insertion and genome manipulation in C. elegans Fang-Jung Yang*, Chiao-Nung Chen*, Tiffany Chang*, Ting-Wei Cheng, Ni-Chen Chang, Chia-Yi Kao, Chih-Chi Lee, Yu-Ching Huang, Shih-Peng Chan#, John Wang# |
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