Strain Information
| Name | BN69 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | npp-5(tm3039)/mIn1 [mIs14 dpy-10(e128)] II; bqIs51 ltIs37 IV. |
| Description | bqIs51 [pie-1p::GFP::npp-5 + unc-119(+)] IV. ltIs37 [(pAA64) pie-1p::mCherry::his-58 + unc-119(+)] IV. Expresses GFP::NPP-5 and mCherry in the germ line. Homozygous deletion chromosome balanced by GFP- and dpy-10-marked inversion. tm3039 homozygotes are viable but produce progengy that are primarily Lva or Lvl; bqIs51 transgene rescues the npp-5(tm3039) phenotype. Heterozygotes are WT with relatively dim pharyngeal GFP signal, and segregate WT dim GFP, Dpy bright GFP (mIn1 homozygotes), and non-GFP tm3039 homozygotes. Pick WT with dim GFP in the pharynx and check for correct segregation of progeny to maintain. Reference: Rodenas E, et al. Mol Biol Cell. 2012 Mar;23(5):930-44. |
| Mutagen | No mutagen |
| Outcrossed | x6 |
| Made by | Eduardo Rodenas |
| Laboratory | BN |
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