Strain Information

Name BN452   View On Wormbase
Species C. elegans
GenotypebqSi189 II; mel-28(bq5[GFP::mel-28]) III.
DescriptionbqSi189 [lmn-1p::mCherry::his-58 + unc-119(+)] II. Strain ubiquitously expresses GFP::MEL-28 from the endogenous mel-28 locus. Transgene was inserted into parental strain N2 using CRISPR/Cas9 and co-conversion of dpy-10. Outcrossed twice to N2 to remove dpy-10. bqSi189 is a single-copy MosSCI insertion into ttTi5605 derived from injection of pBN13. Derived from parental strains BN189 and BN426. Reference: Gomez-Saldivar G, et al. PLoS Genet. 2016 Jun 24;12(6):e1006131.
MutagenCrispr/Cas9
Outcrossedx2
Made byC. Ayuso Garcia
Laboratory BN
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