Strain Information
| Name | BN1117 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | bqSi1030 II; bqSi508 IV. |
| Description | bqSi1030 [hsp16.41p::FRT::mCherry::his-58::FRT::vhhGFP4::zif-1 + unc-119(+)] II. bqSi508 [elt-2p::FLP + unc-119(+)] IV. Can be used for conditional depletion of GFP-tagged proteins in the intestine via anti-GFP nanobody fusion to ZIF1 (mediated by recruited ZIF-1 but NOT requiring ZF1 tags). Mechanism features heat-shock inactive ZIF-1 with FRT sites flanking inactivating sequence and intestinal-specific expression of Cre to remove inactivating sequences to free vhhGFP4::zif-1 GFP degradation. May carry unc-119(ed3) III. Reference: Fragoso-Luna A, et al. 2021 bioRxiv 2021.12.21.473632; doi: https://doi.org/10.1101/2021.12.21.473632 |
| Mutagen | none |
| Outcrossed | x2 |
| Made by | Cristina Ayuso |
| Laboratory | BN |
| Reference | Expanded FLP toolbox for spatiotemporal protein degradation and transcriptomic profiling in C. elegans. Fragoso-Luna A, Ayuso C, Eibl M, Muñoz-Jiménez C, Benes V, Cases I, Askjaer P. doi: 10.1101/2021.12.21.473632. |
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