Strain Information
| Name | BK533 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | ifc-2(qpIs111[gfp::3xflag::ifc-2]) ifa-4(qpIs112[mKate2::3xmyc::ifa-4]) X. |
| Description | GFP::3xFlag tag inserted at N-terminus of endogenous ifc-2 locus. mKate2::3xMyc tag inserted at N-terminus of endogenous ifa-4 locus. Strong GFP signal forming meshwork at lumenal surface of excretory canal cell. Strong GFPand mKate2 signal at lumenal surface of excretory canal cell lumen along entire canal lengths. qpIs112 insertion made in parental strain BK531 qpIs111. Inserted between bases 4515979 and 4515978 of Chom. X (Wormbase WS297 release). Reference: Al-Hashimi H, et al. Genetics. 2018 Oct;210(2):637-652. doi: 10.1534/genetics.118.301078. PMID: 29945901. |
| Mutagen | Crispr/Cas9 |
| Outcrossed | x3 |
| Made by | H. Al-Hashimi |
| Laboratory | BK |
| Reference | Al-Hashimi et al., Genetics, 210:637-652 2018. |
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