Strain Information

Name AWR54   View On Wormbase
Species C. elegans
Genotypelin-35(kea7[lin-35p::degron::GFP::lin-35]) I; ieSi57 II.
DescriptionieSi57 [eft-3p::TIR1::mRuby::unc-54 3’UTR + Cbr-unc-119(+)] II. N-terminal degron::GFP tag was inserted into the endogenous lin-35 locus. A single copy insertion of eft-3p::TIR1::mRuby allows auxin-inducible degradation of LIN-35 in somatic tissues. Generated in N2 background. Reference: Willis AR, et al. Sci Adv. 2021 May 5;7(19):eabf3114. PMID: 33952520 [NOTE: lin-35(kea7) is an N-terminal tag; the methods section of the paper incorrectly describes the tag as a C-terminal insertion.]
MutagenNo mutagen
Outcrossedx3
Made byWinnie Zhao
Laboratory AWR
Reference Willis AR, Zhao W, Sukhdeo R, Wadi L, Tamim El Jarkass H, Claycomb JM, Reinke AW. A parental transcriptional response to microsporidia infection induces inherited immunity in offspring. Science Advances 05 May 2021;Vol. 7, no. 19, eabf3114 DOI: 10.1126/sciadv.abf3114.
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