Strain Information
| Name | AV828 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | nbs-1(me102) meIs8/mIn1 [mIs14 dpy-10(e128)] II. |
| Description | meIs8 [pie-1p::GFP::cosa-1 + unc-119(+)] II. Transgene contains a combination of cDNA and genomic sequences of cosa-1 including 212 bp of 3'UTR. GFP is expressed in the adult germline as 6 bright foci per nucleus (one per chromosome pair) from late pachytene through diplotene stages. Homozygous sterile mutation balanced by GFP- and dpy-10-marked inversion. Heterozygotes are WT with relatively dim pharyngeal GFP signal, and segregate WT dim GFP, Dpy bright GFP (mIn1 homozygotes), and non-GFP me103 homozygotes (sterile adult). Pick WT dim GFP and check for correct segregation of progeny to maintain. nbs-1(me103) homozygotes have frayed and aggregated chromosomes at diakinesis of meiosis I. References: Girard C, et al. Proc Natl Acad Sci U S A. 2018 May 8;115(19):E4443-E4452. Yokoo R, et al. Cell. 2012 Mar 30;149(1):75-87. |
| Mutagen | EMS |
| Outcrossed | x4 |
| Made by | Chloe Girard, Baptiste Roelens, Karl A. Zawadzki |
| Laboratory | AV |
| Reference | Interdependent and separable functions of Caenorhabditis elegans MRN-C complex members couple formation and repair of meiotic DSBs Chloe Girard, Baptiste Roelens, Karl A. Zawadzki, Anne M. Villeneuve Proceedings of the National Academy of Sciences May 2018, 115 (19) E4443-E4452; DOI: 10.1073/pnas.1719029115 |
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