Strain Information
| Name | APL622 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | ljfSi2 I; ljfSi39 IV; egl-17(ljf7[egl-17::mNG::3xFlag]) X. |
| Description | ljfSi2 [hlh-8p::2x mKate2::D. melanogaster moesin actin-binding domain::SL2::2x mTurquoise2::PH::3xHA::tbb-2 3'UTR loxN] I. ljfSi39 [myo-3p::egl-15(5a)::SL2::2x mKate2::PH::3xHA::tbb-2 3' UTR lox511i] IV. mNeonGreen::3xFlag tag inserted at the C-terminus of the endogenous egl-17. EGL-15(5a) expression in body wall muscle cells captures free EGL-17, reducing long-range signaling and causing moderately penetrant sex myoblast migration defects. ljfSi2 is a single copy transgene inserted at Chr I:2851088 near ttTi4348 using Cas9-triggered homologous recombination. ljfSi39 is a single copy transgene inserted at Chr IV:4237723 using Cas9-triggered homologous recombination. Reference: Gibney TV & Pani AM. Development. 2025 Aug 21:dev.204802. doi: 10.1242/dev.204802. PMID: 40838367. |
| Mutagen | Crispr/Cas9 |
| Outcrossed | x0 |
| Made by | Theresa Gibney |
| Laboratory | APL |
| Reference | Gibney TV, Pani AM. FGF diffusion is required for directed migration of postembryonic muscle progenitors in C. elegans. Development. 2025 Aug 21:dev.204802. doi: 10.1242/dev.204802. PMID: 40838367. |
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