Strain Information
| Name | AML470 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | juSi164 unc-119(ed3) III; wtfIs458. |
| Description | juSi164 [mex-5p::HIS-72::miniSOG + Cbr-unc-119(+)] III. wtfIs458 [mec-4::Chrimson4.2::SL2::mCherry::unc-54 3' UTR + unc-122::GFP]. Maintain in the covered box to avoid unnecessary exposure to ambient light. Upon blue light treatment (460 nm LED light for 30 min at 4 Hz with 2 mW/mm2), Histone-miniSOG in the germline can induce heritable mutations. Transgenic animals express light-gated ion channel Chrimson and a fluorescent protein mCherry in mechanosensory neurons alongside GFP in coelomocytes. Reference: Liu M, et al. PLoS Biol. 2022 Jan 28;20(1):e3001524. doi: 10.1371/journal.pbio.3001524. PMID: 35089912. |
| Mutagen | none |
| Outcrossed | x0 |
| Made by | Anuj Sharma |
| Laboratory | AML |
| Reference | manuscript in preparation. |
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