Strain Information
| Name | AMJ912 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | jamSi28 II; rde-4(ne301) III. |
| Description | jamSi28 [myo-3p::rde-4(+)::rde-4 3' UTR + unc-119(+)] II. Enables RNAi silencing in body wall muscles in an otherwise rde-4(ne301) background. jamSi28 was integrated into ttTi5605 II in an EG4322 background using MosSCI. Unknown if unc-119(ed9) is still present or homozygous in background. An isolated inserted line was crossed into AMJ8 (juIs73 [unc-25p::GFP] III) to temporarily balance the endogenous rde-4 locus during the subsequent cross; resulting jamSi28 heterozygous males were crossed into WM49 (rde-4(ne301) III). rde-4(ne301) presumed to be homozygous in this strain due to crossing strategy and minimal recombination between rde-3 and juIs73. Reference: Raman P, et al. Nucleic Acids Res. 2017 Aug 21;45(14):8463-8473. doi: 10.1093/nar/gkx484. PMID: 28541563. |
| Mutagen | No mutagen |
| Outcrossed | x0 |
| Made by | Pravrutha Raman |
| Laboratory | AMJ |
| Reference | Raman P, et al. Nucleic Acids Research, 45(14):8463–8473 (2017). https://doi.org/10.1093/nar/gkx484. PMID: 28541563. |
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