Strain Information
| Name | AMJ188 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | rde-4(ne301) III; jamEx3[rgef-1p::rde-4(+)::rde-4 3’UTR & rgef-1p::gfp::unc-54 3’UTR] |
| Description | rde-4(ne301); qtEx136[rgef-1p(F25B3.3)∷unc-22sense; rgef-1p∷unc-22antisense; rgef-1p∷DsRed line 8]; jamEx3[rgef-1p::rde-4(+)::rde-4 3’UTR & rgef-1p::gfp::unc-54 3’UTR] animals were crossed with WM49 (rde-4(ne301) III) animals. rde-4(ne301); jamEx3[rgef-1p::rde-4(+)::rde-4 3’UTR & rgef-1p::gfp::unc-54 3’UTR] animals were isolated. This strain is expected to drive the expression of RDE-4 under the control of the neuronal promoter rgef-1p from an extrachromosomal array in an rde-4(ne301) background, which could result in wider expression of RDE-4 because of the unknown complexity of extrachromosomal arrays. GFP+ animals from this strain show silencing of muscle, intestine, and hypodermal genes by dsRNA. Pick GFP+ animals to maintain the extrachromosomal array. |
| Mutagen | none |
| Outcrossed | x0 |
| Made by | Ed Traver |
| Laboratory | AMJ |
| Reference | Jose, A., Garcia, G. & Hunter, C. Two classes of silencing RNAs move between Caenorhabditis elegans tissues. Nat Struct Mol Biol. 18:1184–1188 (2011). https://doi.org/10.1038/nsmb.2134. Raman, P., Zaghab, S.M., Traver, E.C. & Jose, A.M. The double-stranded RNA binding protein RDE-4 can act cell autonomously during feeding RNAi in C. elegans. Nucleic Acids Research, 45(14):8463–8473 (2017). https://doi.org/10.1093/nar/gkx484. |
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