Strain Information
| Name | AGK234 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | unc-119(ed3) III; niDf199 IV; armEx53. |
| Description | armEx53 [WRM0611aH08 + unc-119(+)]. Pick non-Unc to maintain. unc-119(ed3) was crossed into JU258, the niDf199IV deletion was confirmed by PCR, and these Unc worms were used for bombardment. This strain contains a transgenic array that expresses the WRM0611aH08 fosmid construct. This fosmid contains the niDF199 locus (around 4 kb) that is deleted in the natural C. elegans isolate strain JU258. JU258 worms lack specific 21U-RNAs normally present in N2 worms due to this deletion of the niDF199 locus. Expression of this fosmid construct in JU258 worms restores the expression of the missing 21U-RNAs in the germline, as measured by RT-qPCR. Reference: Cecere G, et al. Mol Cell. 2012 Sep 14;47(5):734-45. |
| Mutagen | No mutagen |
| Outcrossed | x0 |
| Made by | Cecere & Mansisidore |
| Laboratory | AGK |
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