Strain Information
| Name | AFS222 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | zen-4(cle10) IV. |
| Description | Temperature-sensitive embryonic-lethal mutant. Maintain at 15C. Shift L4s to 25C overnight to observe mutant phenotype of embryos produced by adults. Mutants lack a central spindle during early embryonic mitosis and exhibits a late cytokinesis defect (cleavage furrows regress after ingressing in nearly to the center of dividing embryonic cells). This strain can be used for CRISPR-Cas9 co-conversion. There is a causal mis-sense mutation present in zen-4(cle10), GAC to AAC (D520N), and two silent mutations. One silent mutation is a CGA to CGG mutation at codon 523 that creates a recognition site for a Cas9 guide RNA, in order to use zen-4(cle10ts) as a CRISPR/Cas9 co-conversion marker. The other silent mutation is a GCA to GCT mutation at codon 519 that introduces an AluI site for RFLP analysis. Reference: Farboud B, et al. Genetics Early online November 30, 2018; https://doi.org/10.1534/genetics.118.301775. |
| Mutagen | Crispr/Cas9 |
| Outcrossed | x5 |
| Made by | Aaron F. Severson |
| Laboratory | AFS |
| Reference | Strategies for Efficient Genome Editing Using CRISPR-Cas9 Behnom Farboud, Aaron F. Severson, Barbara J. Meyer Early Online November 30, 2018 | https://doi.org/10.1534/genetics.118.301775 |
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