Laboratory Information

NameZX View on WormBase
Allele designationzx
HeadAlexander Gottschalk
InstitutionUniversity of Frankfurt, Frankfurt, Germany
Address Buchmann Institute
Goethe University
Max-vov-Laue-Strasse 15
Frankfurt D-61118
Germany
Website http://www.biochem.uni-frankfurt.de/index.php?id=8
Gene classes nra 

Strains contributed by this laboratory

Strain Genotype Species Description
ZX299 lin-15B&lin-15A(n765) X; zxEx22. C. elegans zxEx22 [myo-3p::ChR2(H134R)::YFP + lin-15(+)].
ZX388 lin-15B&lin-15A(n765) X; zxIs3. C. elegans zxIs3 [unc-47p::ChR2(H134R)::YFP + lin-15(+)]. Strain occasionally segregrates Muv animals; pick non-Muv animals to maintain.
ZX398 lin-15B&lin-15A(n765) X; zxEx32. C. elegans zxEx32 [myo-3p::NpHR + myo-3p::ChR2(H134R)::YFP + lin-15(+)].
ZX422 lin-15B&lin-15A(n765) X; zxEx33. C. elegans zxEx33 [unc-17p::NpHR::eCFP + lin-15(+)].
ZX460 zxIs6 V. C. elegans zxIs6 [unc-17p::ChR2(H134R)::YFP + lin-15(+)] V.
ZX679 zxIs12. C. elegans zxIs12 [F49H12.4P::ChR2(H134R)::mCherry + F49H12.4P::GFP]. Strain expresses Channelrhodopsin-2 (ChR2(H134R)::mCherry) and GFP (as marker) in PVD and AQR, as well as in a non-identified tail neuron, using the F49H12.4 promoter. When grown in the presence of all-trans retinal and illuminated with blue light, animals show strong forward locomotion escape behavior, which can be quantified using locomotion video tracking. The strain can be used to analyze function of PVD cells, and to estimate the role of specific genes in the function of this polymodal nociceptor, downstream of depolarization via ChR2. All-trans retinal needs to be added with OP50 bacteria when seeding plates to render ChR2 functional. References: Husson S, et al. Curr Biol. 2012 May 8;22(9):743-52. Smith CJ, et al. Neuron. 2013 Jul 24;79(2):266-80. Cohen E, et al. Molecular and Cellular Neuroscience 2104 59C: 85-96.
ZX819 lite-1(ce314); zxIs12. C. elegans zxIs12 [F49H12.4P::ChR2(H134R)::mCherry + F49H12.4P::GFP]. Strain expresses Channelrhodopsin-2 (ChR2(H134R)::mCherry) and GFP (as marker) in PVD and AQR, as well as in a non-identified tail neuron, using the F49H12.4 promoter (see also ZX679). When grown in the presence of all-trans retinal and illuminated with blue light, animals show strong forward locomotion escape behavior, which can be quantified using locomotion video tracking. The strain can be used to analyze function of PVD cells, and to estimate the role of specific genes in the function of this polymodal nociceptor, downstream of depolarization via ChR2. All-trans retinal needs to be added with OP50 bacteria when seeding plates to render ChR2 functional. This strain is in lite-1(ce314) background, which eliminates the photophobic behavioral response that will be startled by blue light when longer light stimuli are used (>1s). To not confuse the photophobic behavior induced by LITE-1, with the PVD evoked escape behavior, this strain is needed for experiments with prolonged photostimulation. References: Husson S, et al. Curr Biol. 2012 May 8;22(9):743-52. Smith CJ, et al. Neuron. 2013 Jul 24;79(2):266-80. Cohen E, et al. Molecular and Cellular Neuroscience 2104 59C: 85-96.
ZX899 lite-1(ce314) X; ljIs123; zxEx621. C. elegans ljIs123 [mec-4p::ChR2(H134R)::YFP(codon-optimized) + unc-122p::RFP]. zxEx621 [glr-1p::Mac::mCherry + elt-2p::GFP]. Pick animals with robust GFP expression in intestinal nuclei to maintain.
This laboratory hasn't submitted any alleles to the CGC.