Laboratory Information
| Name | ZX View on WormBase |
|---|---|
| Allele designation | zx |
| Head | Alexander Gottschalk |
| Institution | University of Frankfurt, Frankfurt, Germany |
| Address | Buchmann Institute Goethe University Max-vov-Laue-Strasse 15 Frankfurt D-61118 Germany |
| Website | http://www.biochem.uni-frankfurt.de/index.php?id=8 |
| Gene classes | nra |
Strains contributed by this laboratory
| Strain | Genotype | Species | Description |
|---|---|---|---|
| ZX2604 | sng-1(ok234) X; zxIs127. | C. elegans | zxIs127 [sng-1p::SNG-1::CRY2olig(535) + myo-2p::mCherry]. Strain should be kept in the dark; it is very light-sensitive. Pan-neuronal expression of a truncated variant of Arabidopsis thaliana Cryptochrome-2 fused to synaptogyrin. If illuminated with blue light, synaptic vesicles cluster, resulting in inhibition of synaptic transmission. Synaptic transmission is restored within 15 minutes in the absence of light (ca. 6.5min time constant), resulting in normal wild type behavior afterwards. Reference: Vettkotter D, et al. Nat Commun 13, 7827 (2022). https://doi.org/10.1038/s41467-022-35324-z. PMID: 36535932 |
| ZX299 | lin-15B&lin-15A(n765) X; zxEx22. | C. elegans | zxEx22 [myo-3p::ChR2(H134R)::YFP + lin-15(+)]. |
| ZX388 | lin-15B&lin-15A(n765) X; zxIs3. | C. elegans | zxIs3 [unc-47p::ChR2(H134R)::YFP + lin-15(+)]. Strain occasionally segregrates Muv animals; pick non-Muv animals to maintain. |
| ZX398 | lin-15B&lin-15A(n765) X; zxEx32. | C. elegans | zxEx32 [myo-3p::NpHR + myo-3p::ChR2(H134R)::YFP + lin-15(+)]. |
| ZX422 | lin-15B&lin-15A(n765) X; zxEx33. | C. elegans | zxEx33 [unc-17p::NpHR::eCFP + lin-15(+)]. |
| ZX460 | zxIs6 V. | C. elegans | zxIs6 [unc-17p::ChR2(H134R)::YFP + lin-15(+)] V. |
| ZX679 | zxIs12. | C. elegans | zxIs12 [F49H12.4P::ChR2(H134R)::mCherry + F49H12.4P::GFP]. Strain expresses Channelrhodopsin-2 (ChR2(H134R)::mCherry) and GFP (as marker) in PVD and AQR, as well as in a non-identified tail neuron, using the F49H12.4 promoter. When grown in the presence of all-trans retinal and illuminated with blue light, animals show strong forward locomotion escape behavior, which can be quantified using locomotion video tracking. The strain can be used to analyze function of PVD cells, and to estimate the role of specific genes in the function of this polymodal nociceptor, downstream of depolarization via ChR2. All-trans retinal needs to be added with OP50 bacteria when seeding plates to render ChR2 functional. References: Husson S, et al. Curr Biol. 2012 May 8;22(9):743-52. Smith CJ, et al. Neuron. 2013 Jul 24;79(2):266-80. Cohen E, et al. Molecular and Cellular Neuroscience 2104 59C: 85-96. |
| ZX819 | lite-1(ce314); zxIs12. | C. elegans | zxIs12 [F49H12.4P::ChR2(H134R)::mCherry + F49H12.4P::GFP]. Strain expresses Channelrhodopsin-2 (ChR2(H134R)::mCherry) and GFP (as marker) in PVD and AQR, as well as in a non-identified tail neuron, using the F49H12.4 promoter (see also ZX679). When grown in the presence of all-trans retinal and illuminated with blue light, animals show strong forward locomotion escape behavior, which can be quantified using locomotion video tracking. The strain can be used to analyze function of PVD cells, and to estimate the role of specific genes in the function of this polymodal nociceptor, downstream of depolarization via ChR2. All-trans retinal needs to be added with OP50 bacteria when seeding plates to render ChR2 functional. This strain is in lite-1(ce314) background, which eliminates the photophobic behavioral response that will be startled by blue light when longer light stimuli are used (>1s). To not confuse the photophobic behavior induced by LITE-1, with the PVD evoked escape behavior, this strain is needed for experiments with prolonged photostimulation. References: Husson S, et al. Curr Biol. 2012 May 8;22(9):743-52. Smith CJ, et al. Neuron. 2013 Jul 24;79(2):266-80. Cohen E, et al. Molecular and Cellular Neuroscience 2104 59C: 85-96. |
| ZX899 | lite-1(ce314) X; ljIs123; zxEx621. | C. elegans | ljIs123 [mec-4p::ChR2(H134R)::YFP(codon-optimized) + unc-122p::RFP]. zxEx621 [glr-1p::Mac::mCherry + elt-2p::GFP]. Pick animals with robust GFP expression in intestinal nuclei to maintain. |
This laboratory hasn't submitted any alleles to the CGC.