Laboratory Information
| Name | ZG View on WormBase |
|---|---|
| Allele designation | ia |
| Head | Powell-Coffman, Jo Anne |
| Institution | Iowa State University, Ames, IA |
| Address | Iowa State University 2128 Molecular Biology Building 2437 Pammel Dr Ames 50011-1079 United States |
| Website | http://www.public.iastate.edu/~japc/homepage.html |
| Gene classes | cky hif rhy |
Strains contributed by this laboratory
| Strain | Genotype | Species | Description |
|---|---|---|---|
| ZG119 | unc-119(ed3) III; iaIs7 IV; vhl-1(ok161) X. | C. elegans | iaIs7 [nhr-57p::GFP + unc-119(+)] IV. Over-expression of nhr-57:GFP in vhl-1 mutant background. Reference: Shen C, et al. Genetics. 2006 Nov;174(3):1205-14. |
| ZG120 | unc-119(ed3) III; iaIs7 IV. | C. elegans | iaIs7 [nhr-57p::GFP + unc-119(+)] IV. GFP expression is very weak. Reference: Shen C, et al. Genetics. 2006 Nov;174(3):1205-14. |
| ZG24 | ahr-1(ia3) I. | C. elegans | Homozygous viable with neuronal defects. |
| ZG31 | hif-1(ia4) V. | C. elegans | Healthy and fertile in standard lab conditions, but unable to adapt to 1% oxygen. When hif-1(+) animals are incubated in1% oxygen, >94% will complete embryogenesis and larval development. In contrast, hif-1(ia4) mutants exhibit 66% embryonic lethality and 9% larval lethality in 1% oxygen. The requirement of hif-1 is alleviated if the oxygen level is increased to 2%. The ia4 mutation is a 1231 bp deletion of the second, third, and fourth exons, which encode much of the helix-loop-helix and PAS domains. Analysis of ESTs suggests that there are at least 4 alternatively spliced hif-1 transcripts. The ia4 deletion introduces a frameshift and a premature stop in the three longest forms. |
| ZG443 | iaIs7 IV; egl-9(ia58) V. | C. elegans | iaIs7 contains [nhr-57p::GFP + unc-119(+)]. Egl. Published in Shao, Zhang, and Powell-Coffman Genetics (2009). |
| ZG444 | iaIs7 IV; egl-9(gk277) V. | C. elegans | iaIs7 contains [nhr-57p::GFP + unc-119(+)]. nhr-57p::GFP is expressed at low levels. Superficially wild-type. Published in Shao, Zhang, and Powell-Coffman Genetics (2009). |
| ZG448 | iaIs7 IV; egl-9(ia60) V. | C. elegans | iaIs7 contains [nhr-57p::GFP + unc-119(+)]. Egl. Published in Shao, Zhang, and Powell-Coffman Genetics (2009). |
| ZG449 | iaIs7 IV; egl-9(ia61) V. | C. elegans | iaIs7 contains [nhr-57p::GFP + unc-119(+)]. ia61 was induced by Mos1 mutagenesis. Egl. Published in Shao, Zhang, and Powell-Coffman Genetics (2009). |
| ZG492 | iaIs7 IV; egl-9(ok478) V. | C. elegans | iaIs7 contains [nhr-57p::GFP + unc-119(+)]. Egl. Published in Shao, Zhang, and Powell-Coffman Genetics (2009). |
| ZG494 | unc-119(ed3) III; egl-9(sa307) V; iaIs38. | C. elegans | iaIs38 contains [egl-9p::egl-9::tag + unc-119(+)]. Superficially WT. Published in Shao, Zhang, and Powell-Coffman Genetics (2009). |
| ZG580 | unc-119(ed3) III; iaIs28. | C. elegans | iaIs28 contains [hif-1p::hif-1a::tag + unc-119(+)]. Published in Zhang et al PLoS ONE 4: e6348 (2009). |
| ZG583 | iaIs34. | C. elegans | iaIs34 contains [hif-1p::hif-1a (P621G)::tag + unc-119(+)]. Published in Zhang et al PLoS ONE 4: e6348 (2009). |
| ZG596 | hif-1(ia7) V. | C. elegans | Published in Zhang et al PLoS ONE 4: e6348 (2009). |
| ZG601 | iaIs21. | C. elegans | iaIs21 [gcy-35p::GFP + unc-119(+)]. gcy-35::GFP is expressed in fifteen neurons, all of which also express ahr-1: AQR, PQR, URXR/L, ALNL/R, BDUL/R, SDQL/R, PLML/R, AVM, and two neurons in the tail tentatively identified as PLNL/R. gcy-35::GFP is only transiently expressed in PLML/R during the first larval stage. |
| ZG610 | iaIs25. | C. elegans | iaIs25 [gcy-37p::GFP + unc-119(+)]. gcy-37::GFP is consistently expressed in AQR, PQR, URXL. and URXR neurons. Expression also observed in AVM and two unidentified neurons located in the head. |
| ZG611 | iaIs19. | C. elegans | iaIs19 [gcy-32p::GFP + unc-119(+)]. Expression of gcy-32::GFP is consistenly observed in AQR, PQR, and URX neurons. |
| ZG629 | iaIs22. | C. elegans | iaIs22 [gcy-36p::GFP + unc-119(+)]. gcy-36::GFP is consistently expressed in AQR, PQR, URXL and URXR neurons. |
| ZG686 | unc-119(ed3) III; egl-9(sa307) V; iaEx101. | C. elegans | iaEx101 contains [egl-9p::egl-9(H487A)::tag + unc-119(+)]. Published in Shao, Zhang, and Powell-Coffman Genetics (2009). |
Alleles contributed by this laboratory
| Allele | Type | DNA Change | Protein Change |
|---|---|---|---|
| ia4 | Allele | deletion | |
| ia3 | Allele | deletion | |
| ia58 | Allele | deletion | frameshift |
| ia60 | Transposon insertion | insertion | |
| ia61 | Allele | substitution | frameshift |
| ia7 | Allele | substitution | nonsense |