Laboratory Information
| Name | VH View on WormBase |
|---|---|
| Allele designation | hd |
| Head | Harald Hutter |
| Institution | Simon Fraser University, Burnaby BC, Canada |
| Address | Simon Fraser University Hutter Lab - Biology 8888 University Drive Burnaby V5A 1S6 Canada |
| Website | http://biology.sfu.ca/people/profiles/hutter |
| Gene classes | ast ddr igcm nas ncam nre rig scm |
Strains contributed by this laboratory
| Strain | Genotype | Species | Description |
|---|---|---|---|
| VH1075 | rhIs4 hdIs26 III; fmi-1(rh308) V. | C. elegans | rhIs4 [glr-1p::GFP + dpy-20(+)] III. hdIs26 [odr-2p::CFP + sra-6p::DsRed2] III. Ventral cord cross-over defects. PVQ axons sometimes stop short or leave the ventral cord. Reference: Steimel A, et al. Development. 2010 Nov;137(21):3663-73. |
| VH1160 | ast-1(hd92) II; rhIs4 III; hdEx237. | C. elegans | rhIs4 [glr-1p::GFP + dpy-20(+)] III. hdEx237 [ast-1(+) + rol-6(su1006)]. hd92 arrests as L1 due to pharyngeal differentiation defects; ventral cord midline crossing defects; rescued with ast-1(+) transgene. Rollers. |
| VH1195 | hdIs42. | C. elegans | hdIs42[ast-1::YFP + rol-6(su1006)]. GFP expression in neurons; contains full length AST-1 tagged with GFP at the C terminus. Rollers. |
| VH1312 | nas-6(hd108) IV. | C. elegans | Slow growth. Reduced rate of pumping and abnormal morphology of the grinder in pharynx. Reference: Park JO, et al. BMC Dev Biol. 2010 Jan 28;10:14. |
| VH1348 | nas-7(hd116) II. | C. elegans | Superficially wild-type. Reference: Park JO, et al. BMC Dev Biol. 2010 Jan 28;10:14. |
| VH1565 | rhIs4 hdIs26 III; fmi-1(hd121) V. | C. elegans | rhIs4 [glr-1p::GFP + dpy-20(+)] III. hdIs26 [odr-2p::CFP + sra-6p::DsRed2] III. Ventral cord cross-over defects. PVQ axons sometimes stop short or leave the ventral cord. Reference: Steimel A, et al. Development. 2010 Nov;137(21):3663-73. |
| VH17 | ast-1(rh300) II; rhIs4 III. | C. elegans | rhIs4 [glr-1p::GFP + dpy-20(+)] III. Ventral cord midline crossing defects. |
| VH1940 | cdh-4(hd40) III. | C. elegans | Partially penetrant embryonic and larval lethality. Variable morphological defects. Reference: Schmitz C, et al. Dev Biol. 2008 Apr 15;316(2):249-59. |
| VH254 | pha-1(e2123) III; hdEx81. | C. elegans | hdEx81 [F25B3.3::tau352(PHP) + pha-1(+)]. Maintain at 25C to select for array. Animals become progressively uncoordinated with age. Reference: Brandt R, et al. Neurobiol Aging. 2009 Jan;30(1):22-33. |
| VH255 | pha-1(e2123) III; hdEx82. | C. elegans | hdEx82 [F25B3.3::tau352(WT) + pha-1(+)]. Maintain at 25C to select for array. Animals become progressively uncoordinated with age. Reference: Brandt R, et al. Neurobiol Aging. 2009 Jan;30(1):22-33. |
| VH29 | cdh-4(rh310) rhIs4 III. | C. elegans | rhIs4 [glr-1p::GFP + dpy-20(+)] III. Lateral axons and ventral cord cross-over defects. Partially penetrant embryonic and larval lethality. Reference: Schmitz C, et al. Dev Biol. 2008 Apr 15;316(2):249-59. |
| VH4 | rhIs4 III; zag-1(rh315) IV. | C. elegans | rhIs4 [glr-1p::GFP + dpy-20(+)] III. Hypomorph. Unc. Axon outgrowth defects and misexpression of glr-1::GFP marker. |
| VH624 | rhIs13 V; nre-1(hd20) lin-15B(hd126) X. | C. elegans | rhIs13 [unc-119::GFP + dpy-20(+)]. RNAi hypersensitive, effective RNAi in the nervous system. unc-119::GFP in neurons is almost completely suppressed on anti-GFP RNAi plates. Reduced progeny at 25C (almost sterile). nre-1(hd20) and lin-15B(hd126) seem very closely linked. Maintain at 15C or 20C. |
| VH715 | hdIs17 I; hdIs10 V; nre-1(hd20) lin-15B(hd126) X. | C. elegans | hdIs17 [glr-1::YFP + unc-47::YFP + unc-129::YFP + rol-6(su1006)]. hdIs10 [unc-129::CFP + glr-1::YFP + unc-47::DsRed + hsp-16::rol-6(su1006)]. Rollers. Reduced progeny at 25C (almost sterile). RNAi hypersensitive, effective RNAi in the nervous system. unc-47::DsRed is weak and only visible in adults. hsp-16::rol-6 transgene is not effectively Roll. Maintain at 15 or 20C. |
| VH725 | pha-1(e2123) III; hdEx231. | C. elegans | hdEx231 [C16C10.10::GFP + pha-1(+)]. Ubiquitous expression of glyoxalase-1::GFP. Maintain at 25 C. Reference: Morcos M et al. (2008) Aging Cell 7(2):260-9. |
Alleles contributed by this laboratory
| Allele | Type | DNA Change | Protein Change |
|---|---|---|---|
| hd36 | Allele | deletion | |
| hd30 | Allele | deletion | |
| hd43 | Allele | deletion | |
| hd31 | Allele | deletion | |
| hd1 | Allele | substitution | |
| hd102 | Allele | deletion | |
| hd92 | Allele | ||
| hd108 | Allele | deletion | |
| hd116 | Allele | insertion | |
| hd121 | Allele | substitution | nonsense |
| hd20 | Allele | ||
| hd126 | Allele | substitution | splice_site |