Laboratory Information

NameVH View on WormBase
Allele designationhd
HeadHarald Hutter
InstitutionSimon Fraser University, Burnaby BC, Canada
Address Simon Fraser University
Hutter Lab - Biology
8888 University Drive
Burnaby V5A 1S6
Canada
Website http://biology.sfu.ca/people/profiles/hutter
Gene classes ast  ddr  igcm  nas  ncam  nre  rig  scm 

Strains contributed by this laboratory

Strain Genotype Species Description
VH1075 rhIs4 hdIs26 III; fmi-1(rh308) V. C. elegans rhIs4 [glr-1p::GFP + dpy-20(+)] III. hdIs26 [odr-2p::CFP + sra-6p::DsRed2] III. Ventral cord cross-over defects. PVQ axons sometimes stop short or leave the ventral cord. Reference: Steimel A, et al. Development. 2010 Nov;137(21):3663-73.
VH1160 ast-1(hd92) II; rhIs4 III; hdEx237. C. elegans rhIs4 [glr-1p::GFP + dpy-20(+)] III. hdEx237 [ast-1(+) + rol-6(su1006)]. hd92 arrests as L1 due to pharyngeal differentiation defects; ventral cord midline crossing defects; rescued with ast-1(+) transgene. Rollers.
VH1195 hdIs42. C. elegans hdIs42[ast-1::YFP + rol-6(su1006)]. GFP expression in neurons; contains full length AST-1 tagged with GFP at the C terminus. Rollers.
VH1312 nas-6(hd108) IV. C. elegans Slow growth. Reduced rate of pumping and abnormal morphology of the grinder in pharynx. Reference: Park JO, et al. BMC Dev Biol. 2010 Jan 28;10:14.
VH1348 nas-7(hd116) II. C. elegans Superficially wild-type. Reference: Park JO, et al. BMC Dev Biol. 2010 Jan 28;10:14.
VH1565 rhIs4 hdIs26 III; fmi-1(hd121) V. C. elegans rhIs4 [glr-1p::GFP + dpy-20(+)] III. hdIs26 [odr-2p::CFP + sra-6p::DsRed2] III. Ventral cord cross-over defects. PVQ axons sometimes stop short or leave the ventral cord. Reference: Steimel A, et al. Development. 2010 Nov;137(21):3663-73.
VH17 ast-1(rh300) II; rhIs4 III. C. elegans rhIs4 [glr-1p::GFP + dpy-20(+)] III. Ventral cord midline crossing defects.
VH1940 cdh-4(hd40) III. C. elegans Partially penetrant embryonic and larval lethality. Variable morphological defects. Reference: Schmitz C, et al. Dev Biol. 2008 Apr 15;316(2):249-59.
VH254 pha-1(e2123) III; hdEx81. C. elegans hdEx81 [F25B3.3::tau352(PHP) + pha-1(+)]. Maintain at 25C to select for array. Animals become progressively uncoordinated with age. Reference: Brandt R, et al. Neurobiol Aging. 2009 Jan;30(1):22-33.
VH255 pha-1(e2123) III; hdEx82. C. elegans hdEx82 [F25B3.3::tau352(WT) + pha-1(+)]. Maintain at 25C to select for array. Animals become progressively uncoordinated with age. Reference: Brandt R, et al. Neurobiol Aging. 2009 Jan;30(1):22-33.
VH29 cdh-4(rh310) rhIs4 III. C. elegans rhIs4 [glr-1p::GFP + dpy-20(+)] III. Lateral axons and ventral cord cross-over defects. Partially penetrant embryonic and larval lethality. Reference: Schmitz C, et al. Dev Biol. 2008 Apr 15;316(2):249-59.
VH4 rhIs4 III; zag-1(rh315) IV. C. elegans rhIs4 [glr-1p::GFP + dpy-20(+)] III. Hypomorph. Unc. Axon outgrowth defects and misexpression of glr-1::GFP marker.
VH624 rhIs13 V; nre-1(hd20) lin-15B(hd126) X. C. elegans rhIs13 [unc-119::GFP + dpy-20(+)]. RNAi hypersensitive, effective RNAi in the nervous system. unc-119::GFP in neurons is almost completely suppressed on anti-GFP RNAi plates. Reduced progeny at 25C (almost sterile). nre-1(hd20) and lin-15B(hd126) seem very closely linked. Maintain at 15C or 20C.
VH715 hdIs17 I; hdIs10 V; nre-1(hd20) lin-15B(hd126) X. C. elegans hdIs17 [glr-1::YFP + unc-47::YFP + unc-129::YFP + rol-6(su1006)]. hdIs10 [unc-129::CFP + glr-1::YFP + unc-47::DsRed + hsp-16::rol-6(su1006)]. Rollers. Reduced progeny at 25C (almost sterile). RNAi hypersensitive, effective RNAi in the nervous system. unc-47::DsRed is weak and only visible in adults. hsp-16::rol-6 transgene is not effectively Roll. Maintain at 15 or 20C.
VH725 pha-1(e2123) III; hdEx231. C. elegans hdEx231 [C16C10.10::GFP + pha-1(+)]. Ubiquitous expression of glyoxalase-1::GFP. Maintain at 25 C. Reference: Morcos M et al. (2008) Aging Cell 7(2):260-9.

Alleles contributed by this laboratory

Allele Type DNA Change Protein Change
hd36 Allele deletion
hd30 Allele deletion
hd43 Allele deletion
hd31 Allele deletion
hd1 Allele substitution
hd102 Allele deletion
hd92 Allele
hd108 Allele deletion
hd116 Allele insertion
hd121 Allele substitution nonsense
hd20 Allele
hd126 Allele substitution splice_site