Laboratory Information

NameTWH View on WormBase
Allele designationyyz
HeadKalia, Lorraine
InstitutionToronto Western Hospital, Toronto, ON, Canada
Address Krembil Research Institution
Rm. 8KD-408
60 Leonard Ave.
Toronto M5T 2R1
Canada
Gene classes

Strains contributed by this laboratory

Strain Genotype Species Description
TWH179 hrpr-1(yyz8) I; tmIs905 II; egIs1 IV. C.elegans tmIs905 [dat-1p::alpha-synuclein(A30P) + ges-1p::DsRed] II. egIs1 [dat-1p::GFP]; reportedly maps to LG IV. hrpr-1 also known as hrp-2. yyz8 is a weak loss-of-function allele. Neurodegeneration in ADE neurons. Neurites are shorter in PDE neurons.
TWH211 hrpr-1(yyz8) I; sid-1(pk3321) uIs69 V; egIs1. C.elegans uIs69 [(pCFJ90) myo-2p::mCherry + unc-119p::sid-1]. egIs1 [dat-1p::GFP]. hrpr-1 also known as hrp-2. Derived from parental strain TU3401, which has been reported as showing RNAi response in non-neuronal tissues.
TWH283 hrpr-1(yyz8) I; tmIs905 II; egIs1 IV; yyzSi1. C.elegans tmIs905 [dat-1p::alpha-synuclein(A30P) + ges-1p::DsRed] II. egIs1 [dat-1p::GFP]; reportedly maps to LG IV. yyzSi1 [dat-1p::hrpr-1(genomic)::hrpr-1 3'UTR + HygR]. Transgene expression in dopaminergic neurons rescues yyz8 phenotype. hrpr-1 also known as hrp-2.
TWH334 yyzEx41. C.elegans yyzEx41 [dat-1p::UbG76V::mRuby::T2A::mClover + rol-6(su1006)]. Pick Rollers to maintain. Red and green fluorescence in dopaminergic neurons. TWH336 can be used as a control strain.
TWH335 sid-1(pk3321) uIs69 V; yyzEx41. C.elegans uIs69 [pCFJ90 (myo-2p::mCherry) + unc-119p::sid-1]. yyzEx41 [dat-1p::UbG76V::mRuby::T2A::mClover + rol-6(su1006)]. Pick Rollers to maintain. Red and green fluorescence in dopaminergic neurons. Red fluorescence in pharynx. Derived from parental strain TU3401, which has been reported as showing RNAi response in non-neuronal tissues.
TWH336 yyzEx42. C.elegans yyzEx42 [dat-1p::mRuby::T2A::mClover + rol-6(su1006)]. Pick Rollers to maintain. Red and green fluorescence in dopaminergic neurons. Can be used as a control for Ub::mRuby in dopaminergic neurons to analyze UPS activity.
TWH337 sid-1(pk3321) uIs69 V; yyzEx42. C.elegans uIs69 [pCFJ90 (myo-2p::mCherry) + unc-119p::sid-1]. yyzEx42 [dat-1p::mRuby::T2A::mClover + rol-6(su1006)]. Pick Rollers to maintain. Red and green fluorescence in dopaminergic neurons. Derived from parental strain TU3401, which has been reported as showing RNAi response in non-neuronal tissues.
TWH388 egIs1 IV; yyzSi9. C. elegans egIs1 [dat-1p::GFP]; reportedly maps to LG IV. yyzSi9 [rab-3p::hrp-2(cDNA)::mRuby::hrpr-1 3'UTR + HygR]. Hygromycin resistant. hrpr-1 transgene expression in neurons. hrpr-1 also known as hrp-2.
This laboratory hasn't submitted any alleles to the CGC.