Laboratory Information
| Name | TG View on WormBase |
|---|---|
| Allele designation | gt |
| Head | Anton Gartner |
| Institution | University of Dundee, Dundee, UK |
| Address | Department for Gene Expression and Regulation The University of Dundee Dow Street Dundee DD1 5EH United Kingdom |
| Website | http://sky.lifesci.dundee.ac.uk/lifesciences/gartner/archive/antongartner.htm |
| Gene classes | gen hus tdpo |
Strains contributed by this laboratory
| Strain | Genotype | Species | Description |
|---|---|---|---|
| SLE1 | E. coli [argA, lysA, mcrA, mcrB, IN(rrnD-rrnE)1, lambda-, rcn14::Tn10(DE3 lysogen::lavUV5 promoter -T7 polymerase]. | Escherichia coli | Bacteria. E. coli carrying pAG607 (orn-1 RNAi feeding vector) for SILAC. Arg-, Lys-, AmpR, TetR. argA, lysA, mcrA, mcrB, IN(rrnD-rrnE)1, lambda-, rnc14::Tn10(DE3 lysogen::lavUV5 promoter -T7 polymerase). Resistant to ampicillin and tetracycline. Reference: Larance M, et al. Nat Methods. 2011 Aug 28;8(10):849-51. Biosafety Level: BSL-1. |
| TG11 | cep-1(lg12501) I; unc-119(ed4) III; gtEx2. | C. elegans | gtEx2[CEP-1::GFP + unc-119(+)]. GFP expression in germ line of adult worms (20-40 hours post L4) in the late pacytene area in the nucleus. Also expression in alae and a few nuclei in the pharynx. Compound microscope needed to see staining. Maintain by picking non-Unc. |
| TG12 | cep-1(lg12501) I; unc-119(ed4) III; gtIs1. | C. elegans | gtIs1[CEP-1::GFP + unc-119(+)]. GFP expression in germ line of adult worms (20-40 hours post L4) in the late pacytene area in the nucleus. Also expression in alae and a few nuclei in the pharynx. Compound microscope needed to see staining. |
| TG1540 | gen-1(tm2940) III. | C. elegans | Reference: Bailly AP, et al. PLoS Genet. 2010 Jul 15;6(7):e1001025. Agostinho A, et al. PLos Genetics 2013. |
| TG1568 | fan-1(tm423) IV. | C. elegans | 411 bp deletion removes exons 6-8. Homozygotes are viable, but are hypersensitive to DNA crosslinking. |
| TG1660 | xpf-1(tm2842) II. | C. elegans | Reference: Agostinho A, et al. PLos Genetics 2013. |
| TG1663 | ercc-1(tm1981) I. | C. elegans | Reference: Agostinho A, et al. PLos Genetics 2013. |
| TG1681 | vtIs1 V; tsp-17(gt1681) X. | C. elegans | vtIs1 [dat-1p::GFP + rol-6] V. Rollers. Reference: Masoudi N, et al. PLoS Genet. 2014 Dec 4;10(12):e1004767. |
| TG1749 | unc-119(ed3) III; ltIs37 IV; gtIs60. | C. elegans | gtIs60 [pie-1p::GFP(lap)::orc-1 + unc-119(+)]. ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV. Reference: Sonneville R, et al. J Cell Biol. 2012 Jan 23;196(2):233-46. |
| TG1750 | unc-119(ed3) III; ltIs37 IV, gtIs61. | C. elegans | gtIs61 [pie-1p::GFP(lap)::orc-2 + unc-119(+)]. ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV. Reference: Sonneville R, et al. J Cell Biol. 2012 Jan 23;196(2):233-46. |
| TG1751 | unc-119(ed3) III; ltIs37 IV; gtIs62. | C. elegans | gtIs62 [pie-1p::GFP(lap)::cdc-6 + unc-119(+)]. ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV. Reference: Sonneville R, et al. J Cell Biol. 2012 Jan 23;196(2):233-46. |
| TG1752 | unc-119(ed3) III; gtIs63. | C. elegans | gtIs63 [pie-1p::GFP(lap)::mcm-2 + unc-119(+)]. Reference: Sonneville R, et al. J Cell Biol. 2012 Jan 23;196(2):233-46. |
| TG1753 | unc-119(ed3) III; ltIs37 IV; gtIs64. | C. elegans | gtIs64 [pie-1p::GFP(lap)::mcm-3 + unc-119(+)]. ltIs37 [(pie-1p::mCherry::his-58 + unc-119(+)] IV. Reference: Sonneville R, et al. J Cell Biol. 2012 Jan 23;196(2):233-46. |
| TG1754 | unc-119(ed3) III; ltIs37 IV; gtIs65. | C. elegans | gtIs65 [pie-1p::GFP(lap)::cdc-45 + unc-119(+)]. ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV. Reference: Sonneville R, et al. J Cell Biol. 2012 Jan 23;196(2):233-46. |
| TG1755 | unc-119(ed3) III; ltIs37 IV; gtIs66. | C. elegans | gtIs66 [pie-1p::GFP(lap)::div-1 + unc-119(+)]. ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV. Reference: Sonneville R, et al. J Cell Biol. 2012 Jan 23;196(2):233-46. |
| TG1756 | unc-119(ed3) III; ltIs37 IV. gtIs67. | C. elegans | gtIs67 [pie-1p::GFP(lap)::sld-5 + unc-119(+)]. ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV. Reference: Sonneville R, et al. J Cell Biol. 2012 Jan 23;196(2):233-46. |
| TG1760 | mus-81(tm1937) I. | C. elegans | Reference: Agostinho A, et al. PLos Genetics 2013. |
| TG1791 | ung-1(tm2862) III. | C. elegans | Superficially wild-type. Deletion site verified by PCR. Reference: Meier B, et al. Genome Res. 2014 Oct;24(10):1624-36. |
| TG1792 | helq-1(tm2134) III. | C. elegans | Superficially wild-type. Deletion site verified by PCR. Reference: Volkova NV, et al. Nat. Commun. 2020 May 1;11(1):2169. |
| TG1868 | slx-1(tm2644) I. | C. elegans | Reference: Agostinho A, et al. PLos Genetics 2013. |
| TG1878 | mus-81(tm1937) slx-1(tm2644) I. | C. elegans | Reference: Agostinho A, et al. PLos Genetics 2013. |
| TG1890 | mus-81(tm1937) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III); xpf-1(tm2842) II. | C. elegans | Segregates WT GFP+ heterozygotes, non-GFP mus-81; xpf-1 double homozygotes, very rare GFP+ homozygous hT2, and dead eggs. Maintain by picking wild-type GFP+ to retain balanced strain: 15-25% of mus-81; xpf-1 double homozygotes are viable. unc-119(ed3) has likely been lost through outcrossing, but could still be present in the background. Reference: Agostinho A, et al. PLos Genetics 2013. |
| TG1891 | slx-1(tm2644) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III); xpf-1(tm2842) II. | C. elegans | Segregates WT GFP+ heterozygotes, non-GFP slx-1; xpf-1 double homozygotes, very rare GFP+ homozygous hT2, and dead eggs. Maintain by picking wild-type GFP+ to retain balanced strain: 15-25% of slx-1 xpf-1 double homozygotes are viable. Reference: Agostinho A, et al. PLos Genetics 2013. |
| TG2196 | him-6(ok412) spo-11(me44) IV/nT1 (IV;V). | C. elegans | Him. Heterozygotes are WT and segregate WT, Vul and dead eggs. Maintain by picking WT. Reference: Agostinho A, et al. PLos Genetics 2013. |
| TG2226 | xpc-1(tm3886) IV. | C. elegans | Superficially wild-type. Deletion site verified by PCR. Reference: Meier B, et al. Genome Res. 2014 Oct;24(10):1624-36. |
| TG2228 | polq-1(tm2026) III. | C. elegans | Superficially wild-type. Deletion site verified by PCR. Reference: Muzzini DM, et al. DNA Repair (Amst.) 2008 Jun 1;7(6):941-50. |
| TG2367 | unc-119(ed3) III; gtIs2367/+. | C. elegans | gtIs2367 [pie-1p::GFP(lap)::orc-5 + unc-119(+)]. Maintain by picking non-Unc. Reference: Sonneville R, et al. J Cell Biol. 2012 Jan 23;196(2):233-46. |
| TG2368 | unc-119(ed3) III; ltIs37 IV; gtIs2368. | C. elegans | gtIs2368 [pie-1p::GFP(lap)::rpa-1 + unc-119(+)]. ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV. Reference: Sonneville R, et al. J Cell Biol. 2012 Jan 23;196(2):233-46. |
| TG2394 | cat-2(e1112) II; vtIs1 V; tsp-17(gt1681) X. | C. elegans | vtIs1 [dat-1p::GFP + rol-6(su1006)] V. Rollers. Reference: Masoudi N, et al. PLoS Genet. 2014 Dec 4;10(12):e1004767. |
| TG2401 | dat-1(ok157) III; vtIs1 V; tsp-17(gt1681) X. | C. elegans | vtIs1 [dat-1p::GFP + rol-6(su1006)] V. Many animals roll weakly or not at all, but still express GFP. Reference: Masoudi N, et al. PLoS Genet. 2014 Dec 4;10(12):e1004767. |
| TG2411 | vtIs1 dop-2(vs105) V; tsp-17(gt1681) X. | C. elegans | vtIs1 [dat-1p::GFP + rol-6(su1006)] V. Rollers. Reference: Masoudi N, et al. PLoS Genet. 2014 Dec 4;10(12):e1004767. |
| TG2415 | vtIs1 dop-2(vs105) V; dop-1(vs100) dop-3(vs106) X. | C. elegans | vtIs1 [dat-1p::GFP + rol-6(su1006)] V. Rollers. Reference: Masoudi N, et al. PLoS Genet. 2014 Dec 4;10(12):e1004767. |
| TG2416 | vtIs1 dop-2(vs105) V; dop-1(vs100) dop-3(vs106) tsp-17(gt1681) X. | C. elegans | vtIs1 [dat-1p::GFP + rol-6(su1006)] V. Rollers. Reference: Masoudi N, et al. PLoS Genet. 2014 Dec 4;10(12):e1004767. |
| TG2435 | vtIs1 V. | C. elegans | vtIs1 [dat-1p::GFP + rol-6(su1006)] V. Strain does not roll well, but GFP expression is easily detected. Reference: Nass R, et al. Proc Natl Acad Sci U S A. 2002 Mar 5;99(5):3264-9. Masoudi N, et al. PLoS Genet. 2014 Dec 4;10(12):e1004767. |
| TG2436 | vtIs1 V; tsp-17(tm4995) X. | C. elegans | vtIs1 [dat-1p::GFP + rol-6(su1006)] V. Rollers. [NOTE: tsp-17(tm4995) is the correct allele carried in this strain. The genotype was annotated incorrectly in Masoudi N, et al. (S. Mitani, 11/2016)] Reference: Masoudi N, et al. PLoS Genet. 2014 Dec 4;10(12):e1004767. |
| TG2437 | vtIs1 V; tsp-17(tm5169) X. | C. elegans | vtIs1 [dat-1p::GFP + rol-6(su1006)] V. Rollers. Reference: Masoudi N, et al. PLoS Genet. 2014 Dec 4;10(12):e1004767. |
| TG2452 | mus-81(tm1937) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III); xpf-1(tm2842) II; gtIs2512. | C. elegans | gtIs2512 [pie-1p::his-11::GFP + unc-119(+)]. Segregates WT GFP+ heterozygotes, non-GFP mus-81; xpf-1 double homozygotes, very rare GFP+ homozygous hT2, and dead eggs. Maintain by picking wild-type GFP+ to retain balanced strain: 15-25% of mus-81; xpf-1 double homozygotes are viable. unc-119(ed3) has likely been lost through outcrossing, but could still be present in the background. Reference: Agostinho A, et al. PLos Genetics 2013. |
| TG2454 | slx-1(tm2644) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III); xpf-1(tm2842) II; gtIs2512. | C. elegans | gtIs2512 [pie-1p::his-11::GFP + unc-119(+)]. Segregates WT GFP+ heterozygotes, non-GFP slx-1; xpf-1 double homozygotes, very rare GFP+ homozygous hT2, and dead eggs. Maintain by picking wild-type GFP+ to retain balanced strain: 15-25% of slx-1; xpf-1 double homozygotes are viable. unc-119(ed3) has likely been lost through outcrossing, but could still be present in the background. Reference: Agostinho A, et al. PLos Genetics 2013. |
| TG2519 | rip-1(tm2948) III. | C. elegans | Superficially wild-type. Deletion site verified by PCR. Reference: Taylor MRG, et al. Cell. 2015 Jul 16;162(2):271-286. |
| TG2520 | pole-4(tm4613) II. | C. elegans | Superficially wild-type. Deletion site verified by PCR. Reference: Meier B, et al. Genome Res. 2018 May;28(5):666-675. |
| TG2932 | tdpo-1(gk889420) IV. | C. elegans | Superficially wild-type. Deletion site verified by PCR. Reference: Volkova NV, et al. Nat. Commun. 2020 May 1;11(1):2169. |
| TG2978 | rev-1(gk924750) II. | C. elegans | Superficially wild-type. Deletion site verified by PCR. Reference: Volkova NV, et al. Nat. Commun. 2020 May 1;11(1):2169. |
| TG3320 | apn-1(cxTi10435) II. | C. elegans | Superficially wild-type. Deletion site verified by PCR. Mos transposon insertion into apn-1; insertion site verified by PCR. Reference: Volkova NV, et al. Nat. Commun. 2020 May 1;11(1):2169. |
| TG34 | gld-1(op236) I. | C. elegans | Hypersensitive to cep-1/p53 mediated apoptosis. |
| TG3525 | fnci-1(tm3081) I. | C. elegans | Superficially wild-type. Deletion site verified by PCR. Reference: Volkova NV, et al. Nat. Commun. 2020 May 1;11(1):2169. |
| TG3527 | fncm-1(tm3148) I. | C. elegans | Superficially wild-type. Deletion site verified by PCR. Reference: Volkova NV, et al. Nat. Commun. 2020 May 1;11(1):2169. |
| TG3796 | bub-3(gt2000) II. | C. elegans | Y54G9A.6. Nonsense C to T transition. IR sensitive. To genotype: WT left mismatch primer: GAAACAGGCAACGGAACAC; mutant left mismatch primer: GAAACAGGCAACGGAAACT; right mismatch primer: CTCTTCATCATCTCCTCTCC. WT and mutant amplicon: 407 bp. Reference: Bertolini S, et al. G3 (Bethesda). 2017 Dec 4;7(12):3875-3885. |
| TG38 | aak-2(gt33) X. | C. elegans | Hypersensitive to oxidative stress, heat, and UV. 606 bp deletion from nucleotide 22 of Exon 3 to nucleotide 160 of Intron 3. |
| TG3867 | xpg-1(tm1670) I. | C. elegans | Superficially wild-type. Deletion site verified by PCR. Reference: Meier B, et al. 2020 bioRxiv, https://doi.org/10.1101/2020.06.04.133306 |
| TG3880 | rev-3(gk919715) III. | C. elegans | Superficially wild-type. Deletion site verified by PCR. Reference: Volkova NV, et al. Nat. Commun. 2020 May 1;11(1):2169. |
| TG3967 | bub-3(ok3437) II | C. elegans | Y54G9A.6. IR sensitive. To genotype: External left primer: GTCCCGTTTCCCCCATTTG. External right primer: CTCTTCATCATCTCCTCTCC. Internal right primer: CTCCTCCGAACGCTACTT. External WT amplicon: 1970 bp. External mutant amplicon: 1635 bp. Internal WT amplicon: 1534 bp. Reference: Bertolini S, et al. G3 (Bethesda). 2017 Dec 4;7(12):3875-3885. Kim T, et al. J Cell Biol. 2015 May 25;209(4):507-17. |
| TG3969 | san-1(ok1580) I. | C. elegans | ZC328.4. IR sensitive. External left primer: AACAAGAAGGGGAAGAAAGA. External right primer: TGTCTCATCGAAATCCAACT. Internal Left Sequence: AGGAAGAAACGAGAAAAGCA. External WT amplicon: 1420 bp. External mutant amplicon: 428 bp. Internal WT amplicon: 720 bp. Reference: Bertolini S, et al. G3 (Bethesda). 2017 Dec 4;7(12):3875-3885. |
| TG4094 | unc-119(ed3) III; cxTi10816 IV; otIs433 V; gtEx4094. | C. elegans | otIs433 [dat-1::NLS::RFP + ttx-3::mCherry] V. gtEx4094 [glit-1p::GFP::glit-1 3'UTR + myo-3p::mCherry]. Pick animals with red fluorescence in body muscles to maintain. Transcriptional glit-1 reporter. Reference: Offenburger SL, et al. https://www.biorxiv.org/content/early/2017/10/13/203067. |
| TG4100 | vtIs1 V; glit-1(gt1981) X. | C. elegans | vtIs1 [dat-1p::GFP + rol-6(su1006)] V. Strain does not roll obviously. gt1981 is a point mutation in a highly conserved residue. Hypersensitive to oxidative stress: Increased dopaminergic neurodegeneration after 6-OHDA exposure and increased developmental delay after exposure to rotenone and paraquat. Reference: Offenburger SL, et al. https://www.biorxiv.org/content/early/2017/10/13/203067. |
| TG4103 | ttr-33(gt1983) vtIs1 V. | C. elegans | vtIs1 [dat-1p::GFP + rol-6(su1006)] V. Strain does not roll obviously. Hypersensitive to oxidative stress: Increased dopaminergic neurodegeneration after 6-OHDA exposure and increased developmental delay after exposure to rotenone and paraquat. Reference: Offenburger SL, et al. https://www.biorxiv.org/content/early/2017/10/13/198606. |
| TG4209 | smg-1(gk761853) I. | C. elegans | |
| TG4267 | lem-3(gt3309[eGFP::Stag::lem-3]) I. | C. elegans | GFP tag inserted into endogenous lem-3 locus by CRISPR. Reference: Hong Y, et al. Nat Commun. 2018 Feb 20;9(1):728. |
| TG4281 | unc-119(ed3) III; cxTi10816 IV; gtEx4170. | C. elegans | gtEx4170 [ttr-33p::GFP::ttr-33 3'UTR + myo-2p::mCherry + myo-3p::mCherry]. Pick mCherry+ animals to maintain. Transcriptional ttr-33 reporter. Reference: Offenburger SL, et al. https://www.biorxiv.org/content/early/2017/10/13/198606. |
| TG4298 | lem-3(gt3310[eGFP::STag::lem-3[S192A S194A]]) I. | C. elegans | Endogenous lem-3 locus carries GFP tag and two misense mutations in putative phosphorylation sites [S192 S194]. Homozygous viable, though [S192A S194A] mutants exhibit increased embryonic lethality after irradiation. Reference: Hong Y, et al. Nat Commun. 2018 Feb 20;9(1):728. |
| TG4300 | lem-3(gt3311[eGFP::Stag::lem-3[Y556A G558A]]) I. | C. elegans | Endogenous lem-3 locus carries GFP tag and two misense mutations in conserved residues [Y556A G558A] of GIY-YIG nuclease domain. Homozygous viable, though [Y556A G558A] mutants exhibit increased embryonic lethality after irradiation and abolished localization of GFP::LEM-3 at the midbodies. Reference: Hong Y, et al. Nat Commun. 2018 Feb 20;9(1):728. |
| TG4319 | lem-3(tm3468) I. | C. elegans | Homozygous viable. Increased embryonic lethality after irradiation. |
| TG5 | rad-51(lg8701) IV/nT1 [let-?(m435)] (IV;V). | C. elegans | Heterozygotes are WT and segregate WT and dead eggs. 1/3 of the WT are rad-51(lg8701) homozygotes and will give only dead eggs. |
| TG9 | dpy-13(e184) rad-51(lg8701) IV/nT1 [let-?(m435)] (IV;V). | C. elegans | Heterozygotes are WT and segregate WT, Dpys which give only dead eggs, and dead eggs. |
| XY1054 | cep-1(lg12501) I. | C. elegans | 1213 bp deletion corresponding to bp 30458-31670 on cosmid F52B5. Takes out a large part of the cep-1 open reading frame. |
Alleles contributed by this laboratory
| Allele | Type | DNA Change | Protein Change |
|---|---|---|---|
| gt33 | Allele | deletion |