Laboratory Information
| Name | TAM View on WormBase |
|---|---|
| Allele designation | ram |
| Head | Montgomery, Taiowa |
| Institution | Colorado State University, Fort Collins, CO |
| Address | Colorado State University 200 West Lake Street 1878 Campus Delivery Fort Collins 80523 United States |
| Gene classes |
Strains contributed by this laboratory
| Strain | Genotype | Species | Description |
|---|---|---|---|
| TAM109 | ramTi3 II. | C. elegans | ramTi3 [ubl-1::mCherry::pri-mir-58 + Cbr-unc-119(+)] II. mCherry-based sensor for pri-miR-58 processing. Weak ubiquitous mCherry fluorescence. MosSCI insertion. Previously described as ram3. Reference: Knittel TL, et al. Nat Commun. 2025 Jul 1;16(1):5595. doi: 10.1038/s41467-025-60721-5. PMID: 40595566. |
| TAM127 | drsh-1(syb6628[mCherry::drsh-1]) I. | C.elegans | mCherry tag inserted inserted directly after start codon of endogenous drsh-1 locus, labeling both F26E4.10A.1 and F26E4.10B.1. mCherry fluorescence enriched in nuclei of embryos and germ cells. Reference: Knittel TL, et al. Nat Commun. 2025 Jul 1;16(1):5595. doi: 10.1038/s41467-025-60721-5. PMID: 40595566. |
| TAM129 | pash-1(syb6091[pash-1::GFP]) I. | C.elegans | GFP tag inserted directly before the stop codon of endogenous pash-1 locus. GFP fluorescence enriched in nuclei of embryos and germ cells. Reference: Knittel TL, et al. Nat Commun. 2025 Jul 1;16(1):5595. doi: 10.1038/s41467-025-60721-5. PMID: 40595566. |
| TAM142 | rde-4(syb6665) III. | C.elegans | RNAi-defective. Mild fertility defects. rde-4(syb6665) is an engineered full length deletion (start codon to stop codon) in the endogenous rde-4 locus. Reference: Knittel TL, et al. Nat Commun. 2025 Jul 1;16(1):5595. doi: 10.1038/s41467-025-60721-5. PMID: 40595566. |
| TAM2 | alg-5(ram2[GFP::3xFLAG + LoxP]) I. | C. elegans | alg-5(ram2[GFP::3xFLAG + LoxP]) is a deletion allele of alg-5. The coding region of the alg-5 genomic locus was replaced with GFP::3xFLAG using CRISPR-Cas9. Transgenic animals express GFP::3xFLAG under the control of alg-5 regulatory elements. GFP expression in the germline can be seen under a microscope. Reference: Nucleic Acids Res. 2017;45(15):9093-9107. doi:10.1093/nar/gkx536 PMID: 28645154 |
| TAM201 | rde-1(ram40) V. | C.elegans | RNAi-defective. rde-1(ram40) is an engineered full length deletion (start codon to stop codon) in the endogenous rde-1 locus. Reference: Knittel TL, et al. Nat Commun. 2025 Jul 1;16(1):5595. doi: 10.1038/s41467-025-60721-5. PMID: 40595566. |
| TAM93 | pash-1(ram33[G179R]) I. | C. elegans | Maintain at 15C. Low penetrance protruding vulva and bursting at permissive temperatures. pash-1(ram33[G179R]) is a G754A substitution (relative to the start codon in T22A3.5A.1) that results in a G179R substitution in the PASH-1 WW domain. Reference: Knittel TL, et al. Nat Commun. 2025 Jul 1;16(1):5595. doi: 10.1038/s41467-025-60721-5. PMID: 40595566. |
| TAM94 | pash-1(syb4327[W180A]) I. | C. elegans | Maintain at 15C. Reduced brood size. Low penetrance protruding vulva and bursting. Temperature sensitive-sterility. pash-1(syb4327[W180A]) is a TGG to GCA substitution at position 756 (relative to the start codon in T22A3.5A.1) that results in a W180A substitution in the PASH-1 WW domain. Reference: Knittel TL, et al. Nat Commun. 2025 Jul 1;16(1):5595. doi: 10.1038/s41467-025-60721-5. PMID: 40595566. |
| TAM95 | drsh-1(syb6628[mCherry::drsh-1]) pash-1(syb6091[pash-1::GFP]) I. | C. elegans | mCherry tag inserted inserted directly after start codon of endogenous drsh-1 locus. GFP tag inserted directly before the stop codon of endogenous pash-1 locus. Fluorescence enriched in nuclei of embryos and germ cells. Reference: Knittel TL, et al. Nat Commun. 2025 Jul 1;16(1):5595. doi: 10.1038/s41467-025-60721-5. PMID: 40595566. |
This laboratory hasn't submitted any alleles to the CGC.