Laboratory Information

NameSU View on WormBase
Allele designationjc
HeadJeff Hardin
InstitutionUniversity of Wisconsin-Madison, Madison WI, USA
Address University Of WI Madison
326 Zoology res
1117 W. johnson
Madison 53706
United States
Website http://worms.zoology.wisc.edu/
Gene classes ajm  jam  sorb  srgp  tes  zoo 

Strains contributed by this laboratory

Strain Genotype Species Description
JJ1136 unc-119(e2498) III; zuEx24. C. elegans zuEx24 [hmp-1::GFP + unc-119(+)]. Animals with the duplication are WT. Occasionally pick green hermaphrodites to maintain. zuEx24 transmits at a very high frequency.
NG3124 dsh-2(or302)/mIn1 [mIs14 dpy-10(e128)] II. C. elegans mIs14 [myo-2p::GFP + pes-10p::GFP]. Heterozygotes are WT with relatively dim pharyngeal GFP signal, and segregate WT dim GFP+ heterozygotes, Dpy bright GFP+ (mIn1 homozygotes), and non-GFP or302 homozygotes. Pick WT dim GFP and check for correct segregation of progeny to maintain. or302 homozygotes are GFP- and are Emb, ABar and have EMS spindle misalignment. or302 is a deletion starting at 503 and ending at 1578 of C27A2.6.
NR220 kzIs7. C. elegans kzIs7 [lin-26p::NLS::GFP + rol-6(su1006)]. Maintain by picking Rollers.
NR221 rde-1(ne219) V; kzIs8. C. elegans kzIs8 [lin-26p::NLS::GFP + rol-6(su1006)]. Maintain by picking Rollers.
SU112 hmr-1(zu389)/lin-11(n566) unc-75(e950) I; jcIs1 IV. C. elegans jcIs1 [ajm-1::GFP + unc-29(+) + rol-6(su1006)] IV. Heterozygotes are Rollers and segregate Rollers, Hmr inviable embyros and Egl Unc. ajm-1 was formerly known as jam-1 (Junction Associated Protein) and "the gene encoding the antigen recognized by the monoclonal antibody MH27." jcIs1 consists of pJS191, C45D3 and pRF4. Reference: Koppen M, et al. Nat Cell Biol. 2001 Nov;3(11):983-91.
SU159 ajm-1(ok160) X; jcEx44. C. elegans jcEx44 [ajm-1::GFP + rol-6(su1006)]. Throws Rollers (weak -- some animals appear nearly wild-type) expressing ajm-1::GFP and dead eggs.
SU180 itr-1(jc5) jcIs1 IV. C. elegans jcIs1 [ajm-1::GFP + unc-29(+) + rol-6(su1006)] IV. itr-1(jc5) is cold sensitive. Progeny of homozygotes reared at 15C exhibit 95% maternal effect lethality, while progeny of homozygotes reared at 20C exhibit 15.2% lethality. Arrested embryos are defective in epithelial morphogenesis. ajm-1 was formerly known as jam-1 (Junction Associated Protein) and "the gene encoding the antigen recognized by the monoclonal antibody MH27." jcIs1 consists of pJS191, C45D3 and pRF4.
SU265 jcIs17. C. elegans jcIs17 [hmp-1p::hmp-1::GFP + dlg-1p::dlg-1::DsRed + rol-6(su1006)]. Rollers. References: Zaidel-Bar R, et al. J Cell Biol. 2010 Nov 15;191(4):761-9. Raich WB, et al. Curr Biol. 1999 Oct 21;9(20):1139-46.
SU295 jcIs25. C. elegans jcIs25 [pPE103 (jac-1::GFP) + rol-6(su1006)]. Rollers. Reference: Pettitt et al. 2003. LCB 162:15-22.
SU351 mig-5(rh94)/mIn1 [dpy-10(e128) mIs14] II. C. elegans Heterozygotes are WT with GFP in pharynx. Segregate Dpy and GFP+. mig-5 homozygotes are non-GFP and show a weakly penetrant gonad defect and a fully penetrant QL.d migration defect.
SU352 mig-5(rh147)/mIn1 [dpy-10(e128) mIs14] II. C. elegans Heterozygotes are WT with GFP in pharynx. Segregate Dpy and GFP+. mig-5 homozygotes are non-GFP and show a weakly penetrant gonad defect and a fully penetrant QL.d migration defect.
SU62 unc-44(e1260) lag-1(q385)/zen-4(w35) IV. C. elegans Heterozygotes are WT and segregate WT, Unc L1 lethals and dead eggs (homozygous zen-4(w35)).
SU875 mig-10(jc53[mig-10::mNeonGreen::3xFlag + LoxP]) III. C. elegans Endogenously tagged mig-10. No growth, behavior, or morphological defects observed. Reference: Serre JM & Hardin J. (2022) The Lamellipodin homologue MIG-10 is not essential for dorsal intercalation in the embryonic epidermis of the C. elegans embryo. microPublication Biology. 10.17912/micropub.biology.000522.
SU93 jcIs1 IV. C. elegans jcIs1 [ajm-1::GFP + unc-29(+) + rol-6(su1006)] IV. ajm-1 was formerly known as jam-1 (Junction Associated Protein) and "the gene encoding the antigen recognized by the monoclonal antibody MH27." jcIs1 consists of pJS191, C45D3 and pRF4. Reference: Koppen M, et al. Nat Cell Biol. 2001 Nov;3(11):983-91.
temp_name258 dsh-1(or301)/mIn1 [dpy-10(e128) mIs14] II. Actually carries dsh-2(or302) instead of or301

Alleles contributed by this laboratory

Allele Type DNA Change Protein Change
jc36 Allele deletion
jc5 Allele