Laboratory Information
| Name | SU View on WormBase |
|---|---|
| Allele designation | jc |
| Head | Jeff Hardin |
| Institution | University of Wisconsin-Madison, Madison WI, USA |
| Address | University Of WI Madison 326 Zoology res 1117 W. johnson Madison 53706 United States |
| Website | http://worms.zoology.wisc.edu/ |
| Gene classes | ajm jam sorb srgp tes zoo |
Strains contributed by this laboratory
| Strain | Genotype | Species | Description |
|---|---|---|---|
| JJ1136 | unc-119(e2498) III; zuEx24. | C. elegans | zuEx24 [hmp-1::GFP + unc-119(+)]. Animals with the duplication are WT. Occasionally pick green hermaphrodites to maintain. zuEx24 transmits at a very high frequency. |
| NG3124 | dsh-2(or302)/mIn1 [mIs14 dpy-10(e128)] II. | C. elegans | mIs14 [myo-2p::GFP + pes-10p::GFP]. Heterozygotes are WT with relatively dim pharyngeal GFP signal, and segregate WT dim GFP+ heterozygotes, Dpy bright GFP+ (mIn1 homozygotes), and non-GFP or302 homozygotes. Pick WT dim GFP and check for correct segregation of progeny to maintain. or302 homozygotes are GFP- and are Emb, ABar and have EMS spindle misalignment. or302 is a deletion starting at 503 and ending at 1578 of C27A2.6. |
| NR220 | kzIs7. | C. elegans | kzIs7 [lin-26p::NLS::GFP + rol-6(su1006)]. Maintain by picking Rollers. |
| NR221 | rde-1(ne219) V; kzIs8. | C. elegans | kzIs8 [lin-26p::NLS::GFP + rol-6(su1006)]. Maintain by picking Rollers. |
| SU1085 | tes-1(jc110[mScarlet-1::FLAG::tes-1 + LoxP511]) IV. | C. elegans | mScarlet and FLAG tags inserted into endogenous tes-1 locus by CRISPR/Cas9 genome editing. Reference: Lynch AM, et al. Curr Biol. 2022 Dec 5;32(23):5189-5199.e6. doi: 10.1016/j.cub.2022.10.045. PMID: 36384139. |
| SU112 | hmr-1(zu389)/lin-11(n566) unc-75(e950) I; jcIs1 IV. | C. elegans | jcIs1 [ajm-1::GFP + unc-29(+) + rol-6(su1006)] IV. Heterozygotes are Rollers and segregate Rollers, Hmr inviable embyros and Egl Unc. ajm-1 was formerly known as jam-1 (Junction Associated Protein) and "the gene encoding the antigen recognized by the monoclonal antibody MH27." jcIs1 consists of pJS191, C45D3 and pRF4. Reference: Koppen M, et al. Nat Cell Biol. 2001 Nov;3(11):983-91. |
| SU159 | ajm-1(ok160) X; jcEx44. | C. elegans | jcEx44 [ajm-1::GFP + rol-6(su1006)]. Throws Rollers (weak -- some animals appear nearly wild-type) expressing ajm-1::GFP and dead eggs. |
| SU180 | itr-1(jc5) jcIs1 IV. | C. elegans | jcIs1 [ajm-1::GFP + unc-29(+) + rol-6(su1006)] IV. itr-1(jc5) is cold sensitive. Progeny of homozygotes reared at 15C exhibit 95% maternal effect lethality, while progeny of homozygotes reared at 20C exhibit 15.2% lethality. Arrested embryos are defective in epithelial morphogenesis. ajm-1 was formerly known as jam-1 (Junction Associated Protein) and "the gene encoding the antigen recognized by the monoclonal antibody MH27." jcIs1 consists of pJS191, C45D3 and pRF4. |
| SU265 | jcIs17. | C. elegans | jcIs17 [hmp-1p::hmp-1::GFP + dlg-1p::dlg-1::DsRed + rol-6(su1006)]. Rollers. References: Zaidel-Bar R, et al. J Cell Biol. 2010 Nov 15;191(4):761-9. Raich WB, et al. Curr Biol. 1999 Oct 21;9(20):1139-46. |
| SU295 | jcIs25. | C. elegans | jcIs25 [pPE103 (jac-1::GFP) + rol-6(su1006)]. Rollers. Reference: Pettitt et al. 2003. LCB 162:15-22. |
| SU351 | mig-5(rh94)/mIn1 [dpy-10(e128) mIs14] II. | C. elegans | Heterozygotes are WT with GFP in pharynx. Segregate Dpy and GFP+. mig-5 homozygotes are non-GFP and show a weakly penetrant gonad defect and a fully penetrant QL.d migration defect. |
| SU352 | mig-5(rh147)/mIn1 [dpy-10(e128) mIs14] II. | C. elegans | Heterozygotes are WT with GFP in pharynx. Segregate Dpy and GFP+. mig-5 homozygotes are non-GFP and show a weakly penetrant gonad defect and a fully penetrant QL.d migration defect. |
| SU62 | unc-44(e1260) lag-1(q385)/zen-4(w35) IV. | C. elegans | Heterozygotes are WT and segregate WT, Unc L1 lethals and dead eggs (homozygous zen-4(w35)). |
| SU875 | mig-10(jc53[mig-10::mNeonGreen::3xFlag + LoxP]) III. | C. elegans | Endogenously tagged mig-10. No growth, behavior, or morphological defects observed. Reference: Serre JM & Hardin J. (2022) The Lamellipodin homologue MIG-10 is not essential for dorsal intercalation in the embryonic epidermis of the C. elegans embryo. microPublication Biology. 10.17912/micropub.biology.000522. |
| SU896 | hmp-1(jc58[hmp-1::mScarlet-1 + Lox511]) V. | C.elegans | mScarlet tag inserted into endogenous hmp-1 locus by CRISPR/Cas9 genome editing. Reference: Serre JM, et al. PLoS Genet. 2023 Mar 3;19(3):e1010507. doi: 10.1371/journal.pgen.1010507. PMID: 36867663. |
| SU93 | jcIs1 IV. | C. elegans | jcIs1 [ajm-1::GFP + unc-29(+) + rol-6(su1006)] IV. ajm-1 was formerly known as jam-1 (Junction Associated Protein) and "the gene encoding the antigen recognized by the monoclonal antibody MH27." jcIs1 consists of pJS191, C45D3 and pRF4. Reference: Koppen M, et al. Nat Cell Biol. 2001 Nov;3(11):983-91. |
| SU955 | tes-1(jc71[mNG::tes-1 + LoxP]) IV. | C. elegans | mNeonGreen and FLAG tags inserted into endogenous tes-1 locus by CRISPR/Cas9 genome editing. Reference: Lynch AM, et al. Curr Biol. 2022 Dec 5;32(23):5189-5199.e6. doi: 10.1016/j.cub.2022.10.045. PMID: 36384139. |
Alleles contributed by this laboratory
| Allele | Type | DNA Change | Protein Change |
|---|---|---|---|
| jc36 | Allele | deletion | |
| jc5 | Allele |