Laboratory Information

NamePY View on WormBase
Allele designationoy
HeadPiali Sengupta
InstitutionBrandeis University
Address Biology Volen 206
415 south st, lab supply receiving
Waltham 02453
United States
Website http://elegans.swmed.edu/CeRD/search.cgi?re=sengupta&Search=Search
Gene classes alr  osta  snd  sns  srtx 

Strains contributed by this laboratory

Strain Genotype Species Description
KJ461 cng-1(jh111) V. C. elegans
KJ5559 tax-4(p678) III; cng-1(jh111) V. C. elegans
KJ5560 cng-3(jh113) IV; cng-1(jh111) V. C. elegans
KJ5561 tax-4(p678) III; cng-3(jh113) IV. C. elegans
KJ5562 tax-4(p678) III; cng-3(jh113) IV; cng-1(jh111) V. C. elegans
PY1058 oyIs14 V; lin-15B&lin-15A(n765) X. C. elegans oyIs14 [sra-6::GFP + lin-15(+)].
PY1133 unc-130(oy10) II. C. elegans Slighty Dpy. Slighty Unc. Ventral clear patch due to distal tip cell migration defects. Ectopic expression of AWA neuronal markers.
PY12101 oyIs96. C.elegans oyIs96 [gcy-8p::FlincG3 gcy-8p::myr::TagRFP + unc-122p::dsRed]. Red fluorescence in coelomocytes and faint green fluorescence in AFD head neurons. cGMP fluorescent sensor (FlincG3) expression in AFD neurons allows visualization of AFD signaling. Generated in N2 background. Reference: Extrachromosomal array used in the generation of this strain detailed in https://doi.org/10.1534/genetics.119.302392.
PY1260 ttx-1(oy26) oyIs17 V. C. elegans oyIs17 [gcy-8p::GFP + lin-15(+)] V. Thermotaxis defective. Reduced gcy-8::GFP expression in AFD at 25C.
PY1283 ttx-1(oy29) oyIs17 V. C. elegans oyIs17 [gcy-8p::GFP + lin-15(+)] V. Thermotaxis defective. Reduced gcy-8::GFP expression in AFD at 25C.
PY1322 oyIs18 X. C. elegans oyIs18 [gcy-8::GFP]. GFP in AFD.
PY1463 oyEx45. C. elegans oyEx45 [nhr-82::GFP + coelomocyte::GFP]. N2 line injected. Maintain by picking GFP+.
PY1466 oyEx38. C. elegans oyEx38 [nhr-77::GFP, coelomocyte::GFP]. N2 line injected. Maintain by picking GFP+.
PY1479 kin-29(oy38) X. C. elegans Animals are small, developmentally delayed, and have reduced expression of certain olfactory receptors. oy38 is a complex rearrangement of kin-29 genomic sequences.
PY1539 ref-1(oy40) II. C. elegans WT phenotype.
PY1589 cmk-1(oy21) IV. C. elegans Thermotaxis defective.
PY1598 alr-1(oy42) X. C. elegans Previously called sns-10(oy42).
PY2223 mef-2(oy65) I; kin-29(oy39) kyIs104 X. C. elegans kyIs104 [str-1::GFP]. AWB expression of GFP. mef-2 suppresses the phenotypes of kin-29 (small body size, hypersensitivity to dauer pheromone, reduced expression of the str-1 chemoreceptor in the AWB olfactory neurons). WT looking.
PY2285 kin-29(oy39) hda-4(oy59) kyIs104 X. C. elegans kyIs104 [str-1::GFP]. AWB expression of GFP. hda-4 suppresses the phenotypes of kin-29 (small body size, hypersensitivity to dauer pheromone, reduced expression of the str-1 chemoreceptor in the AWB olfactory neurons). WT looking.
PY2289 mef-2(oy63) I; kin-29(oy39) kyIs104 X. C. elegans kyIs104 [str-1::GFP]. AWB expression of GFP. mef-2 suppresses the phenotypes of kin-29 (small body size, hypersensitivity to dauer pheromone, reduced expression of the str-1 chemoreceptor in the AWB olfactory neurons). WT looking.
PY2417 oyIs44 V. C. elegans oyIs44 [odr-1::RFP + lin-15(+)]. Bright RFP in AWB and AWC.
PY3019 alr-1(oy56) X. C. elegans Previously called sns-10(oy56).
PY6523 srbc-66(tm2943) V. C. elegans Superficially wild-type. Reduced dauer formation in response to specific ascarosides. Reference: Kim K, et al. Science. 2009 Nov 13;326(5955):994-8.
PY6560 srbc-64(tm1946) I. C. elegans Superficially wild-type. Reduced dauer formation in response to specific ascarosides. Reference: Kim K, et al. Science. 2009 Nov 13;326(5955):994-8.
PY7502 oyIs85. C. elegans oyIs85 [ceh-36p::TU#813 + ceh-36p::TU#814 + srtx-1p::GFP + unc-122p::DsRed]. TU#813 and TU#814 are split caspase vectors (Chalfie Lab) subcloned downstream of the ceh-36 promoter. AWC ablated in this strain. Defective thermotaxis. Expression of dsRed in coelomocytes. GFP expression in AFD. Reference: Beverly M, Anbil S, Sengupta P. J Neurosci. 2011 Aug 10;31(32):11718-27.
PY7505 oyIs84. C. elegans oyIs84 [gpa-4p::TU#813 + gcy-27p::TU#814 + gcy-27p::GFP + unc-122p::DsRed]. TU#813 and TU#814 are split caspase vectors (Chalfie Lab) subcloned downstream of the gpa-4 promoter and gcy-27 promoter, respectively. ASI is ablated in this strain. Increased dauer formation. Expression of dsRed in coelomocytes. Reference: Beverly M, Anbil S, Sengupta P. J Neurosci. 2011 Aug 10;31(32):11718-27.

Alleles contributed by this laboratory

Allele Type DNA Change Protein Change
oy44 Allele deletion
oy10 Allele
oy26 Allele substitution
oy29 Allele
oy38 Allele deletion
oy40 Allele substitution
oy21 Allele substitution nonsense
oy42 Allele deletion
oy65 Allele substitution
oy39 Allele substitution
oy59 Allele substitution nonsense
oy63 Allele substitution
oy56 Allele substitution splice_site