Laboratory Information

NamePJ View on WormBase
Allele designationj
HeadJacobson, Lew
InstitutionUniversity of Pittsburgh, PA
Address Department of Biological Sciences University of Pittsburgh Pittsburgh, PA 15260

Website http://www.pitt.edu/~biology/Dept/Frame/Faculty/jacobson.htm
Gene classes cad 

Strains contributed by this laboratory

Strain Genotype Species Description
PD55 tra-3(e1107) IV; ccIs55 V. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V. Strain PD55 is transformed with plasmid pPD9.10 (which has a non-nuclear unc-54::lacZ fusion and a copy of the sup-7(st5) gene).
PJ1 cad-1(j1) II. C. elegans Small. Egg retainer. Little or no reproduction at 25C. Gut transparent. 90% reduced cathepsin.
PJ1002 unc-13(e51) I; ccIs55 V. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V. Unc.
PJ1009 unc-51(e369) ccIs55 V. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V.
PJ1015 unc-29(e1072) I; ccIs55 V. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V. Unc.
PJ1017 egl-43(n1079) II; ccIs55 V. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V. Maintain at 25C.
PJ1022 him-8(e1489) IV; ccIs55 V. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V.
PJ1030 ccIs55 V; unc-18(e81) X. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V. Unc. Slow growing.
PJ1034 lev-1(e211) IV; ccIs55 V. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V. Resistant to 1 mM levamisole.
PJ1036 unc-38(e264) I; him-8(e1489) IV; ccIs55 V. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V. Him. Resistant to 1 mM levamisole.
PJ1037 unc-54(e190) I; ccIs55 V. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V.
PJ1039 unc-47(e307) III; ccIs55 V. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V. Unc. Slight shrinking when poked.
PJ104 cad-1(j1) unc-52(e669) II. C. elegans Unc-progressive paralysis. Reduced cathepsin.
PJ1043 tpa-1(k501) IV; ccIs55 V. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V. Superficially wild-type. Resistant to PMA.
PJ1044 unc-25(e156) III; ccIs55 V. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V. Recoils slightly when prodded; poor backward movement.
PJ1045 ccIs55 V; unc-2(e55) X. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V. Slow moving Kinkers. Slow growing.
PJ1046 cha-1(p1182) IV; ccIs55 V. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V. Unc lethal at 25C. Difficult to score <25C.
PJ105 cad-1(j1) II; daf-4(e1364) III. C. elegans Very small, almost Dpy. Temperature sensitive dauer constitutive. Many dauers at 16C. Reduced cathepsin.
PJ1054 unc-68(r1162) ccIs55 V. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V. unc-68 channel null.
PJ1055 cha-1(p1182) IV; unc-68(r1162) ccIs55 V. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V. Slow movement at 25C; might not curl like cha-1 typically does. Very slow movement at 20C. unc-68 channel null.
PJ1062 let-60(n1046) IV; ccIs55 V. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V.
PJ1063 let-60(ga89) IV; ccIs55 V. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V. Temperature sensitive. Nearly WT at 15C. At 20C the animals are 18% Muv and brood size is 88. At 25C the animals are 57% Muv and are almost sterile (brood size is 6).
PJ1065 let-60(n2021) IV; ccIs55 V. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V. Vul/Egl. ras loss-of-signal.
PJ1069 let-60(sy93) IV; ccIs55 V. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V. Dominant Vul allele, however, worms appear to be Egl and have multiple pseudovulvae (due to sup-7??).
PJ1070 clr-1(e1745) II; ccIs55 V. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V. Clear. Grows very poorly at 25C.
PJ1076 ccIs55 V; gap-1(ga133) X. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V.
PJ1077 let-60(ga89) IV; lwIs16 X. C. elegans lwIs16 [act-4::lacZ] X. Temperature sensitive gain-of-function allele of ras. At high temperatures worms become Clr. Should also become Muv- not noted. Maintain at 16C.
PJ1078 clr-1(e1745) II; ccIs55 V; egl-15(n1783) X. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V. Non-Egl. Non-Scrawny. Class IV egl-15 mutation. Supresses the temperature-sensitive Clr phenotype.
PJ1080 age-1(hx546) II; ccIs55 V. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V.
PJ1085 soc-2(n1774) IV; ccIs55 V. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V.
PJ1092 lin-45(sy96) IV; ccIs55 V. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V.
PJ1093 let-60(ga89) IV; ccIs55 V; gap-1(n1329) X. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V. Strain is viable at 15-25C. let-60(ga89) is temperature sensitive; however, with the gap-1 in the background the animals still appear somewhat Clr and Muv even at low temperatures.
PJ1099 lin-45(sy96) let-60(ga89) IV; ccIs55 V. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V. Vul. Non-Clr at 25C. Poor growers (sub-viable?) at 25C.
PJ1100 him-1(e879) I; let-60(ga89) IV; ccIs55 V. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V. Temperature sensitive. Nearly WT at 15C. At 20C the animals are 18% Muv and brood size is 88. At 25C the animals are 57% Muv and almost sterile (brood size is 6). Males appear to mate poorly - not quantitatively measured but very poor success with matings.
PJ1105 mek-2(ku114) I; let-60(ga89) IV; ccIs55 V. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V. Occasional bags and L1 lethality. ga89 is temperature sensitive. Maintain at 16C.
PJ1107 soc-2(n1774) let-60(ga89) IV; ccIs55 V. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V. Appear to have DV and bags with some frequencey. Appear to give Clr phenotype at 16C. Occasional Muv seen. Very frequently sterile due to lack of gonad development. Maintain at 16C.
PJ1109 mpk-1(n2521) III; let-60(ga89) IV; ccIs55 V. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V.
PJ1110 clr-1(e1745) II; lin-45(sy96) IV; ccIs55 V. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V. sy96 appears to suppress the Clr phenotype of e1745. Lots of Bags and larval lethals.
PJ1114 clr-1(e1745) II; mpk-1(n2521) III; ccIs55 V. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V. clr-1 is termperature-sensitive.
PJ1115 gaIs37 IV; ccIs55 V. C. elegans gaIs37 [Ef1a::Dmek hs::mpk-1] IV. ccIs55 [unc-54::lacZ + sup-7(st5)] V. At 20C, 99% of the worms are WT. At 25C, close to 100% of the worms are Muv. Also, a heat-shock at the L2 stage can produce a 80-90% Muv phenotype.
PJ1121 unc-4(e120) let-23(sa62) II; ccIs55 V. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V. Unc. Muv.
PJ1124 mek-2(ku114) I; clr-1(e1745) II; ccIs55 V. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V. Egl. Bags at high frequency. Semi-Clr.
PJ1126 clr-1(e1745) II; ccIs55 V; sem-5(n1779) X. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V. clr-1 is temperature-sensitive. n1779 suppresses clr-1.
PJ1132 daf-18(e1375) IV; ccIs55 V. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V. Dauer defective. May make bags of worms at low frequency??
PJ1134 ccIs55 V; pdk-1(mg142) X. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V. No visible phenotype (may be smallish??). Dominant suppressor of daf-c phenotype of age-1.
PJ1145 ccIs55 V; njEx38. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V. njEx38 [unc-54p::daf-2(+) + goa-1p::GFP + rol-6(su1006)]. Maintain by picking Rollers. Slight Egl.
PJ1146 daf-2(m41) III; ccIs55 V; pdk-1(mg142) X. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V. Gain of function allele of pdk-1.
PJ1153 clr-1(e1745) II; let-756(s2613) unc-32(e189) III; ccIs55 V. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V. Homozygous viable and fertile, but slow-growing, transparent, and small (but not scrawny). Coiler Unc. clr-1 is temperature-sensitive.
PJ1154 clr-1(e1745) II; ccIs55 V; egl-17(n1377) X. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V. Egl - moderate to severe bloating. 30% make bags of worms. Clr.
PJ1155 let-756(s2613) unc-32(e189) III; ccIs55 V; egl-17(n1377) X. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V. Egg laying defective. Moderate to severe bloating. >50% make bags of worms.
PJ1156 sqt-1(sc13) age-1(mg44) II; ccIs55 V; mgEx499. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V. mgEx499 [unc-54p::age-1 + mec-7p::GFP]. Left-hand rollers. Long and thin. Maintain at 16C.
PJ1158 clr-1(e1745) II; let-756(s2613) unc-32(e189) III; ccIs55 V; egl-17(n1377) X. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V. Egl: moderate to severe bloating. Clr-1 is ts. Homozygous viable and fertile, but slow-growing, transparent, and small (but not scrawny). Coiler Unc.
PJ1162 ccIs55 V; unc-1(e719) pdk-1(sa680) X. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V. Unc - recessive kinker. Daf-c at 25C. Egl, Clumpy, Lon, low brood size. Dauers do not recover when moved to 15C in the presence of food. Daf-c, Lon, Clumpy, and fertility defects can be rescued maternally.
PJ1164 cha-1(p1182) IV; ccIs55 V; jIs1. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V. jIs1 [myo-3::GFP + rol-6(su1006)]. jIs1 likely maps to LGI, LGII, or LGX. Rollers; not all animals roll well.
PJ1166 daf-2(m41) III; ccIs55 V; njEx38. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V. njEx38 [unc-54p::daf-2(+) + goa-1p::GFP + rol-6(su1006)]. Maintain by picking Rollers. Arrest as dauers at 25C. Maintain at 15C.
PJ1182 unc-43(n498) IV; ccIs55 V; njEx38. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V. njEx38 [unc-54p::daf-2(+) + goa-1p::GFP + rol-6(su1006)]. Rollers. Pick Rollers to maintain. unc-43 gain-of-function. Progressive paralysis.
PJ1185 daf-5(e1386) II; daf-4(m592) III; ccIs55 V. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V. Maintain at 15C. Small size and weak lacZ staining at 25C. daf-4 dauer defects are suppressed by daf-5, but not the small size.
PJ1190 sma-6(e1482) II; ccIs55 V. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V. Small, not temperature sensitive. Good lacZ staining.
PJ1192 daf-1(m40) IV; iwIs16 X. C. elegans iwIs16 [act-4::lacZ] X. Maintain at 15C. Temperature-sensitive dauer constitutive. 100% dauer formation at 25C; poor dauer recovery at 15C. Maternal effect. Good lacZ expression in spermatheca, but weak in body wall.
PJ1194 daf-14(m77) IV; ccIs55 V. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V. Maintain at 15C. Temperature-sensitive; constitutive dauer formation at 25C.
PJ1195 daf-8(e1393) I; ccIs55 V. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V. Maintain at 15C. Temperature-sensitive; constitutive dauer formation at 25C.
PJ1196 daf-7(m62) III; ccIs55 V. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V. Maintain at 15C. Temperature-sensitive; constitutive dauer formation at 25C. NOTE: m62 is an amber allele of daf-7, which is well suppressed by sup-7 in ccIs55 at 16C.
PJ1199 daf-7(m62) III; ccIs55 V. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V. Maintain at 15C. Constitutive dauer formation at 25C.
PJ1209 unc-129(ev557) IV; ccIs55 V. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V. Unc.
PJ1215 dbl-1(wk70) V; iwIs16 X. C. elegans iwIs16 [act-4::lacZ] X. Good lacZ expression in spermatheca, but weak in muscle.
PJ1224 sma-2(e502) III; ccIs55 V. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V. Small body size. Slow growth. Strong lacZ staining in body wall.
PJ1225 sma-3(wk28) III; ccIs55 V. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V. Small body size, slow growth. Strong lacZ staining in body wall.
PJ1227 sma-4(e729) III; ccIs55 V. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V. Small body size. Slow growth. Strong lacZ staining in body wall.
PJ1228 daf-4(m592) III; ccIs55 V. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V. Maintain at 15C. Leaky dauer constitutive -- ~95% dauer formation at 25C. Small body size at 20C. Strong lacZ staining in body wall at 15C; no staining at 25C.
PJ1254 ccIs55 V; csEx52. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V. csEx52 [hsp::lin-45AA + sur-5::GFP]. Array is unstable; pick GFP+ to maintain. Severe Unc.
PJ1256 unc-51(e369) ccIs55 V; csEx52. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V. csEx52 [hsp::lin-45AA + sur-5::GFP]. Array is unstable; pick GFP+ to maintain.
PJ1257 daf-4(m592) III; unc-51(e369) ccIs55 V. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V. Small. Unc. Most progeny shifted to 26C form dauers.
PJ1258 daf-1(m213) IV; ccIs55 V. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V. Most progeny shifted to 26C form dauers. Some dauer formation at 20C.
PJ1259 daf-1(m402) IV; ccIs55 V. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V. Most progeny shifted to 26C form dauers. Some dauer formation at 20C.
PJ1263 gaIs37 IV; unc-51(e369) ccIs55 V. C. elegans gaIs37 [EF1a::Dmek + hs::mpk-1] IV. ccIs55 [unc-54::lacZ + sup-7(st5)] V. Unc. MuV with some frequency at 25C. Low frequency of tail defects at all temps. Appear more Dpy at 25C than 15C.
PJ1270 unc-43(n408) IV; ccIs55 V; csEx52. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V. csEx52 [hsp::lin-45AA + sur-5::GFP]. Unc slow. Array is unstable, pick GFP+ to maintain.
PJ1271 daf-4(m592) III; ccIs55 V; daf-3(e1376) X. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V. Maintain at 15C. daf-4 is temperature-sensitive and dauer formation (not body size) phenotype is suppressed by daf-3. Dauers will form only when plates are crowded, starved, and maintained at 26C. Animals are small at 25C.
PJ1272 daf-4(m592) III; daf-18(e1375) IV; ccIs55 V. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V. Abnormal-looking dauers form at 25C.
PJ1276 daf-8(e1393) I; daf-14(m77) IV; ccIs55 V. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V. Temperature-sensitive. Some dauer formation at 16C.
PJ1277 ccIs4251 I; unc-51(e369) ccIs55 V. C. elegans ccIs4251 [(pSAK2) myo-3p::GFP::LacZ::NLS + (pSAK4) myo-3p::mitochondrial GFP + dpy-20(+)] I. ccIs55 [unc-54::lacZ + sup-7(st5)] V. GFP expression in nuclei and mitochondria of muscle cells.
PJ1283 clr-1(e1745) II; unc-51(e369) ccIs55 V. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V. Clr fairly well suppressed by unc-51.
PJ1290 clr-1(e1745) II; unc-43(e408) IV; ccIs55 V. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V. Very slow movement. Clr phenotyope is difficult to score.
PJ1305 unc-43(n498j038) IV; ccIs55; njEx38. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V. njEx38 [unc-54p::daf-2(+) + goa-1p::GFP + rol-6(su1006)]. Rollers. Pick Rollers to maintain. unc-43 gain-of-function suppressed; not markedly small. Egl-d. Lethal @ 25C; short L1's do not survive. No GFP expression.
PJ1700 daf-2(m41) III; unc-51(e369) ccIs55 V. C. elegans ccIs55 [unc-54::lacZ + sup-7(st5)] V. Unc -- very slow. Grows very slowly.
PJ2 cad-1(j1) II. C. elegans 90% reduced cathepsin D. Cleaner genetic background than PJ1 cad-1(j1); this strain has been outcrossed 2X.
PJ801 jDf1/mnC1 [dpy-10(e128) unc-52(e444)] II. C. elegans Heterozygotes are Unc (Paralyzed adult) and segregate more Uncs, DpyUncs and dead eggs. Growth slow. Pick Unc to maintain. Crossover suppressed.
PJ803 jDf2/mnC1 [dpy-10(e128) unc-52(e444)] II. C. elegans Heterozygotes are Unc (Paralyzed adult) and segregate more Unc, DpyUnc and dead eggs. Pick Unc to maintain. Balanced well.
PJ821 unc-4(e120) jDf4/unc-4(e120) let-257(mn235) II. C. elegans Heterozygotes are Unc and segregate Unc, dead eggs and UncLets. Lethal in larval development. Also reference 710.
temp_name292 unc-25(e156) III; ccIs55 V. PJ Does not carry e156

Alleles contributed by this laboratory

Allele Type DNA Change Protein Change
j1 Allele