Laboratory Information

NameNP View on WormBase
Allele designationcd
HeadFares, Johnny
InstitutionUniversity of Arizona, Tucson, AZ
Address Department of Molecular and Cellular Biology The University of Arizona Life Sciences South 531 P.O. Box 210106 Tucson, AZ 85721-0106

Website http://www.mcb.arizona.edu/facultyResearchDetail.cfm?id=fares
Gene classes cup  mtm  der 

Strains contributed by this laboratory

Strain Genotype Species Description
GH534 mrp-4(cd8) X. C. elegans WT strain. cd8 will suppress the embryonic and larval lethality of cup-5(zu223).
GS1826 dpy-20(e1282) IV; arIs36. C. elegans arIs36 [(pJF43) hsp::ssGFP + dpy-20(+) + pBluescript SK+]. GFP is secreted into the pseudocoelom upon heat shock and is taken up by coelomocytes. Not known where arIs36 is attached. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.
GS1912 arIs37 I; dpy-20(e1282) IV. C. elegans arIs37 [myo-3p::ssGFP + dpy-20(+)] I. GFP is secreted from muscle cells. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.
GS2477 arIs37 I; cup-5(ar465) III; dpy-20(e1282) IV. C. elegans arIs37 [myo-3p::ssGFP + dpy-20(+)] I. cup-5 is defective in endocytosis by coelomocytes. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.
GS2478 arIs37 I; dpy-20(e1282) IV; cup-8(ar466) V. C. elegans arIs37 [myo-3p::ssGFP + dpy-20(+)] I. cup-8 is defective in endocytosis by coelomocytes. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.
GS2479 arIs37 I; dpy-20(e1282) IV; cup-9(ar467) V. C. elegans arIs37 [myo-3p::ssGFP + dpy-20(+)] I. cup-9 is defective in endocytosis by coelomocytes. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.
GS2484 arIs37 I; dpy-20(e1282) IV; cup-11(ar472) X. C. elegans arIs37 [myo-3p::ssGFP + dpy-20(+)] I. cup-11 is defective in endocytosis by coelomocytes. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.
GS2495 arIs37 I; dpy-20(e1282) IV; mtm-9(ar479) V. C. elegans arIs37 [myo-3p::ssGFP + dpy-20(+)] I. Coelomocyte endocytosis defect. GFP accumulates in body cavity. MTM-9=Y39H10A.3 mtm-9 pka cup-10. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.
GS2526 arIs37 I; mca-3(ar492) dpy-20(e1282) IV. C. elegans arIs37 [myo-3p::ssGFP + dpy-20(+)] I. mca-3 is defective in endocytosis by coelomocytes. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.
GS2527 arIs37 I; mca-3(ar493) dpy-20(e1282) IV. C. elegans arIs37 [myo-3p::ssGFP + dpy-20(+)] I. mca-3 is defective in endocytosis by coelomocytes. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.
GS2532 arIs37 I; cup-3(ar498) II; dpy-20(e1282) IV. C. elegans arIs37 [myo-3p::ssGFP + dpy-20(+)] I. cup-3 is defective in endocytosis by coelomocytes. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.
GS2555 cup-2(ar506) arIs37 I; dpy-20(e1282) IV. C. elegans arIs37 [myo-3p::ssGFP + dpy-20(+)] I. cup-2 is defective in endocytosis by coelomocytes. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.
GS2643 arIs37 I; cup-5(ar465) III; dpy-20(e1282) IV. C. elegans arIs37 [myo-3p::ssGFP + dpy-20(+)] I. cup-5(ar465) is defective in endocytosis by coelomocytes. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.
NP1054 unc-119(ed3) III; cdIs97. C. elegans cdIs97 [pcc1::mCherry::cup-5 + ttx-3::GFP + unc-119(+)]. Ballistic transformation. mCherry::CUP-5 expressed in front coelomocyte promoter.
NP1086 unc-119(ed3) III; cdIs113. C. elegans cdIs113 [pcc1::mCherry::rab-5 + ttx-3::GFP + unc-119(+)]. Ballistic transformation. mCherry::RAB-5 expressed in front coelomocyte promoter.
NP1129 unc-119(ed3) III; cdIs131. C. elegans cdIs131 [pcc1::GFP::rab-5 + myo-2p::GFP + unc-119(+)]. Ballistic transformation. GFP::RAB-5 expressed in front coelomocyte promoter.
NP1154 unc-119(ed3) III; cdIs141. C. elegans cdIs141[pcc1::mCherry::rab-7 + ttx-3::GFP + unc-119(+)]. Ballistic transformation. mCherry::RAB-7 expressed in front coelomocyte promoter.
NP1360 arIs37 I; cup-14(cd31) II. C. elegans arIs37 [myo-3p::ssGFP + dpy-20(+)] I. myo-3p::ssGFP is a secreted GFP that is taken up by coelomocytes. Reference: Gee, K et al. (2017) G3 7: 991.
NP147 cdIs10. C. elegans cdIs10 [pcc1::cup-4::GFP + rol-6(su1006)]. Rollers.
NP704 unc-119(ed3) III; cdIs28. C. elegans cdIs28 [pcc1::mRFP::TRAM + unc-119p::ttx-3::GFP].
NP705 unc-119(ed3) III; cdIs29. C. elegans cdIs29 [pcc1::GFP::TRAM + unc-119p::ttx-3::GFP].
NP738 unc-119(ed3) III; cdIs36. C. elegans cdIs36 [pcc1::C31E10.7::GFP + myo-2p::GFP + unc-119(+)]. Ballistic transformation. C31E10.7::GFP (smooth ER marker) expressed in front coelomocyte promoter.
NP744 unc-119(ed3) III; cdIs39 X. C. elegans cdIs39 [pcc1::GFP::rme-1(271alpha1) + myo-2p::GFP + unc-119(+)].
NP745 unc-119(ed3) III; cdIs40. C. elegans cdIs40 [pcc1::GFP::cup-5 + myo-2p::GFP + unc-119(+)]. Ballistic transformation. cup-5::GFP expressed in front coelomocyte promoter.
NP822 unc-119(ed3) III; cdIs54. C. elegans cdIs54 [pcc1::MANS::GFP + myo-2p::GFP + unc-119(+)]. Ballistic transformation. Mannosidasell::GFP (Golgi marker) expressed in front coelomocyte promoter.
NP871 unc-119(ed3) III; cdIs66. C. elegans cdIs66 [pcc1::GFP::rab-7 + myo-2p::GFP + unc-119(+)]. GFP::rab-7 expressed in front of coelomocyte promoter.
NP878 unc-119(ed3) III; cdIs73. C. elegans cdIs73[RME-8::mRFP + ttx-3::GFP + unc-119(+)]. Ballistic transformation. RME-8::mRFP expressed in front coelomocyte promoter.
NP898 cdIs80. C. elegans cdIs80 [pcc1::PLCd::GFP + rol-6(su1006)]. Rollers.
NP941 unc-119(ed3) III; cdIs85. C. elegans cdIs85 [pcc1::2xFYVE::GFP + myo-2p::GFP + unc-119(+)]. Ballistic transformation. 2xFYVE(Hrs)::GFP expressed in front coelomocyte promoter.

Alleles contributed by this laboratory

Allele Type DNA Change Protein Change
cd8 Allele substitution nonsense