Laboratory Information

NameMQ View on WormBase
Allele designationqm
HeadSiegfried Hekimi
InstitutionMcGill University, Montreal PQ, Canada
Address 1205 rue Docteur Penfield, Stewart Biology Building W-5/26
Hekimi Lab
Montreal H3A1B1
Canada
Website http://hekimilab.mcgill.ca/
Gene classes clk  coq  ctb  cux  dsc  gop  hap  inx  mad  mal  mau  mud  mum  nlt 
opu  ora  stl  sto  toc  tpk 

Strains contributed by this laboratory

Strain Genotype Species Description
CB4876 clk-1(e2519) III. C. elegans Maternally rescued. Homozygous e2519 worms coming from homozygous mothers develop slowly. Adult rhythmic behaviors are also slowed.
MQ1050 daf-16(m26) I; isp-1(qm150) IV. C. elegans Slow development.
MQ1236 clk-1(e2519) III; rte-5(qm197) X. C. elegans qm197 suppresses the L2 arrest and sterility of clk-1(e2519) on UQ- bacteria.
MQ125 clk-2(qm37) III. C. elegans Maternal effect slow growth. Lethal at 25C. Grow at 20C or 15C. See also WBPaper00002456.
MQ130 clk-1(qm30) III. C. elegans Maternally rescued slow growing strain. Grows better at 15C. See also WBPaper00002456.
MQ131 clk-3(qm38) II. C. elegans Maternal effect slow growth. See also WBPaper00002456.
MQ1333 nuo-6(qm200) I. C. elegans Slow biological timing (pharyngeal pumping, defecation, development). May grow better at 15C. Reference: Yang W & Hekimi S (2010) Aging Cell March 19.
MQ1343 dsc-1(qm133) X. C. elegans Pale. Short defecation cycle length. Expulsion defective.
MQ1395 clk-1(e2519) III; rte-1(qm199) X. C. elegans qm199 suppresses the growth arrest and sterility of clik-1(e2519) on ubi- bacteria.
MQ1403 rte-2(qm210) I; clk-1(e2519) III. C. elegans qm210 suppresses the growth arrest and sterility of clik-1(e2519) on UQ- bacteria.
MQ1407 clk-1(e2519) III; rte-4(qm213) X. C. elegans qm213 suppresses the growth arrest and sterility of clik-1(e2519) on ubi- bacteria.
MQ1408 clk-1(e2519) III; rte-3(qm211) X. C. elegans qm211 suppresses the growth arrest and sterility of clik-1(e2519) on ubi- bacteria.
MQ141 dpy-17(e164)/clk-1(e2519) clk-2(qm37) III. C. elegans Hets are WT and segregate WT, Dpys and maternally rescued clk double mutants (WT). To study clk double mutants, pick WT worms singly onto plates. Maternally rescued clk-1 clk-2 worms segregate very slow hatching and developing progeny and no Dpys. Double mutants do not give a viable strain but rather die out over a few generations.
MQ1444 dsc-1(tm241) X. C. elegans Pale. Short defecation cycle length. Expulsion defective.
MQ149 mau-6(qm50) V. C. elegans Maternal effect uncoordinated. Progressive paralysis leading to full paralysis and rapid death after the 4th larval moult. Some embryonic and larval lethality.
MQ1766 sod-2(ok1030) I; sod-5 (tm1146) sod-1(tm783) II; sod-4(gk101) III; sod-3(tm760) X. C. elegans Normal lifespan. Increased sensitivity to oxidative stress, osmotic stress, cold stress, and heat stress. Slow development, slow physiological rates (thrashing, defecation), and reduced fertility. van Raamsdonk J & Hekimi S. Proc Natl Acad Sci U S A. 2012 Apr 10;109(15):5785-90.
MQ177 mau-7(qm56) IV. C. elegans Maternal effect uncoordinated. Lethargic, kinky jerky, ratchet-like movements in reverse. Constipated due to frequent absence of the expulsion contraction. 20% embryonic lethality. 16% embryonic lethality.
MQ200 mud-1(qm21) III. C. elegans Maternal effect Uncoordinated and Dumpy. High degree of embryonic and larval lethality. Dpy phenotype only partially resuced. Hatchlings are of variable length, with poor movement. Adults are short and show kinky uncoordination.
MQ210 mau-4(qm45) X. C. elegans Maternal effect uncoordinated. Stiff posterior body. Severly Unc when attempting to move backwards. Partially wasted posterior body. Some embryonic and larval lethality.
MQ223 clk-3(qm38) II; gro-1(e2400)/dpy-17(e164) III. C. elegans Develops slowly (at the clk-3(qm38) rate). Segregates 1/4 Dpy. gro-1(e2400) is maternally rescued. clk-3; gro-1 doubles throw no Dpy and their progeny develop very slowly. To maintain strain pick WT worms singly onto plates and score progeny.
MQ224 clk-3(qm38) II; clk-1(qm30)/dpy-17(e164) III. C. elegans Develops slowly (clk-3(qm38) rate). clk-1(qm30) is maternally rescued. Segregates 1/4 Dpy progeny. To study clk-3; clk-1 doubles place WT worms singly onto plates-the clk doubles will throw no Dpys and their progeny develop extremely slowly (about 3 days to hatch and 10 days for postembryonic development)-they will be sterile when they finally become adults.
MQ225 clk-3(qm38) II; clk-2(qm37) III. C. elegans Maternally rescued slow growth. Post-embryonic development takes approximately 1.5 days longer then WT at 20C ( clk-2(qm37) is a ts embryonic lethal). Maintain at or below 20C.
MQ228 mad-2(qm62) I. C. elegans Maternal effect dumpy. Adults are short but larvae are not. Animals are lethargic but display hyperactive foraging movement and very rapid pumping when stimulated by light touch. Hermaphrodite and male tails are abnormal. Very slow development. High degree of embryonic and larval lethality.
MQ251 mau-1(e2507) V. C. elegans Maternal effect uncoordinated.
MQ29 mau-3(qm10) IV. C. elegans Maternal effect uncoordinated. Progressive paralysis. Abnorml muscle anatomy. Some embryonic and larval lethality.
MQ4 mau-2(qm4) I. C. elegans Maternal effect uncoordinated. Egg-laying defective. Some larval lethality. Full zygotic rescue. Almost complete maternal rescue, no maternal rescue of egg-laying defect. Neuroanatomical defects. Short excretory canals.
MQ415 age-1(hx546) rrf-3(b26) II; gro-1(e2400) III. C. elegans Slow growing. Not fertile at 25C. Daf-c at 27C.
MQ420 mal-4(qm36) II. C. elegans Maternally rescued variable abnormal. Some embryonic lethality and a high degree of larval lethality. Worms often have a hypertrophic left side of the head, generally anterior of the terminal bulb.
MQ452 aptf-2(qm27) IV. C. elegans Dorsal protrusions on head and tail. Head frequently twisted. 30% of embryos die. 86% of larvae die. 44% of adult survivors show a mutant phenotype. Full zygotic and maternal rescue.
MQ464 emb-4(qm31) V. C. elegans Maternal-effect morphologically abnormal. Variably deformed; frequent hypertrophic ventral side of the head. This strain has a high degree of embryonic and larval lethality. No zygotic rescue and full maternal rescue. Strict maternal effect. PKA mal-2.
MQ465 mum-1(qm32) IV. C. elegans Variably deformed with no prominent single feature. Deformed pharynx. Very severly Unc: from strongly kinky to complete paralysis. Neuroanatomical defects. Abnormal excretory system. Abnormal gonads. 32% of embyros die. 80% of larvae die. 100% of adult survivors show a mutant phenotype.
MQ466 pigv-1(qm34) I. C. elegans Maternally rescued variable abnormal. Some embryonic lethality and a very high level of larval lethality. Head frequently deformed because the buccal cavity does not open at the tip of the head but ventrally, dorsally or laterally.
MQ467 mum-3(qm46) III. C. elegans Variably deformed with no prominent single feature. Deformed pharynx. Very severly Unc: from strongly kinky to complete paralysis. Neuroanatomical defects. Abnormal excretory system. Abnormal gonads. 15% of embyros die. 26% of larvae die. 100% of adult survivors show a mutant phenotype. Full zygotic and maternal rescue.
MQ468 hmp-2(qm39) I. C. elegans Maternally rescued Dpy. Fully zygotically rescued but only partially maternally rescued. Some embryonic lethality and a high degree of larval lethality. Very poor embryonic elongation. Hatchlings are short and deformed. In later stages the anterior half of the body is short but well developed while the posterior is thin.
MQ470 rop-1(pk93) V. C. elegans No visible phenotype. Severely decreased levels of the RoRNP-associated CeY RNA. Higher frequency of mutant 5S rRNA molecules into rop-1 ribosomes. See also WBPaper00003694.
MQ471 mum-2(qm33) IV. C. elegans Variably deformed with no prominent single feature. Deformed pharynx. Very severly Unc: from strongly kinky to complete paralysis. Neuroanatomical defects. Abnormal excretory system. Abnormal gonads. 5% of embyros die. 54% of larvae die. 82% of adult survivors show a mutant phenotype.
MQ475 mau-5(qm17) III. C. elegans Maternal effect uncoordinated. Progressively worsening sluggishness and saccidic forward movement. Egg laying defective. Abnormal muscle anatomy. Some embryonic and larval lethality.
MQ499 mad-3(qm64) V. C. elegans Maternal effect Dpy. High level of embryonic and larval lethality. Lethal at 25C. Males are Dpy.
MQ513 daf-2(e1370) clk-1(e2519) III. C. elegans Daf-c at 25C. Slower growing than clk-1(e2519).
MQ523 mau-8(qm57) IV. C. elegans Maternal effect uncoordinated. Lethargic, kinky jerky, ratchet-like movement in reverse. Constipated due to frequent absence of the expulsion contraction. 15% embryonic lethality. 16% larval lethality.
MQ524 dpy-17(e164)/gro-1(e2400) clk-2(qm37) III. C. elegans Hets are WT and segregate WT, Dpys and maternally rescued GroClks (WT). To study gro-1 clk-2 doubles, pick WT worms singly onto plates. Maternally rescued gro-1 clk-2 worms segregate very slow hatching and developing progeny and no Dpys. Double mutants do not give a viable strain but rather die out after a few generations.
MQ770 tpk-1(qm162) III. C. elegans Maternal effect slow growing.
MQ809 dpy-17(e164) dec-7(qm166) III. C. elegans Dpy. Fast defecation.
MQ853 mau-2(qm160) I. C. elegans 100% Unc. 100% Egl. 15% Lid.
MQ876 daf-2(e1370) III; isp-1(qm150) IV. C. elegans Slow development rate. Dauer constitutive at 25C. Grow at 15C.
MQ887 isp-1(qm150) IV. C. elegans Slow development and behavior. Grows better at 15C.
MQ920 dsc-4(qm182) IV. C. elegans Defecation suppressor of clk-1(qm30).
MQ989 isp-1(qm150) IV; ctb-1(qm189). C. elegans Slow post-embryonic development and behavior.
MQ992 coq-3(qm188)/dpy-4(e1166) IV. C. elegans Heterozygotes are WT and segregate WT, Dpys, and Steriles.
temp_name22 clk-1(qm11)III. has same lesion as e2519, qm11 lost-deleted 5/7/96

Alleles contributed by this laboratory

Allele Type DNA Change Protein Change
qm150 Allele substitution
qm197 Allele substitution
qm37 Allele substitution
qm30 Allele deletion
qm38 Allele
qm200 Allele substitution
qm133 Allele substitution
qm199 Allele substitution
qm210 Allele substitution
qm213 Allele substitution
qm211 Allele substitution
qm50 Allele
qm56 Allele
qm21 Allele
qm45 Allele
qm62 Allele
qm10 Allele
qm4 Allele substitution
qm36 Allele
qm27 Allele
qm31 Allele substitution nonsense
qm32 Allele
qm34 Allele
qm46 Allele
qm39 Allele
qm33 Allele
qm17 Allele
qm64 Allele
qm57 Allele substitution
qm162 Allele substitution
qm166 Allele
qm160 Allele deletion
qm182 Allele substitution
qm189 Allele substitution
qm188 Allele deletion