Laboratory Information

NameKK View on WormBase
Allele designationit
HeadKemphues, Ken
InstitutionCornell University, Ithaca, NY
Address 107 Biotechnology Building Department of Molecular Biology and Genetics Cornell University Ithaca, NY 14853

Website http://www.mbg.cornell.edu/cals/mbg/research/kemphues-lab/
Gene classes frl  inft  mei  mel  nop  ogr  par 

Strains contributed by this laboratory

Strain Genotype Species Description
KK112 mel-8(b309) unc-4(e120) sqt-1(sc13)/mnC1 [dpy-10(e128) unc-52(e444)] II. C. elegans mel-8 is a maternal effect lethal. WT heterozygotes (sc13 recessive) segregate WT, MelUncSqt (sick and small), mnC1 dpy-10 unc-52 homozygotes (paralyzed Dpys). Rescued by male mating.
KK114 daf-7(e1372) par-2(it5) III. C. elegans Temperature sensitive-grow at 15C. Par at 25C. Strict maternal effect lethal.
KK1216 par-3(it298[par-3::GFP]) III. C. elegans Superficially wild-type. Made in N2 background.
KK1218 par-3(it300[par-3::mCherry]) III. C. elegans Superficially wild-type. Made in N2 background.
KK1228 pkc-3(it309[GFP::pkc-3]) II. C. elegans Superficially wild-type. Note that double homozygous mutants of pkc-3(it309) with par-2(it315) exhibited partially penetrant and variable maternal effect lethality and maternal effect sterility that was stronger than it315 alone. Thus, although the GFP tag on pkc-3 shows no effect in an otherwise wild-type background, it does seem to somewhat compromise the activity of the protein it tags. Made in N2 background.
KK1248 par-6(it319[par-6::GFP]) I. C. elegans Superficially wild-type. Made in N2 background.
KK1254 par-2(it315[mCherry::par-2]) III. C. elegans par-2(it315) exhibits a weak maternal effect sterility, suggesting that the tag reduces the protein activity. Note, however, that double homozygous mutants of it315 with pkc-3(it309) or par-6(it310) exhibited partially penetrant and variable maternal effect lethality and maternal effect sterility that was stronger than it315 alone. Thus, although the GFP tags on pkc-3 and par-6 show no effect in an otherwise wild-type background, they do seem to somewhat compromise the activity of the proteins they tag. Made in N2 background.
KK1262 par-1(it324[par-1::GFP::par-1 exon11a]) V. C. elegans Superficially wild-type. Made in N2 background.
KK1273 par-2(it328[GFP::par-2]) III. C. elegans Superficially wild-type. Made in N2 background.
KK157 mel-9(b293) II. C. elegans Maternal effect lethal mutation. Temperature sensitive-grow at 15C. Some growth at 20C. Does not grow at 25C. Rescued by male mating.
KK184 par-4(it47) V. C. elegans Strict maternal effect lethal. Temperature sensitive-grow at 15C. Par at 20C and 25C.
KK204 sma-4(e729) mab-5(e1239) unc-36(e251) III. C. elegans Small and Unc.
KK237 lon-1(e185) par-3(e2074) III; sDp3 (III;f). C. elegans WT sDp3-bearing animals segregate WT and lon-1 par-3 homozygotes that lay eggs that don't hatch. par-3 exhibits mildly ts penetrance in homozygotes. Strict maternal effect. Received new stock 3/01.
KK241 lon-1(e185) par-3(it62) III; sDp3 (III;f). C. elegans Animals with the Duplication are WT. Animals which have lost the Duplication are Lon and throw dead eggs.
KK255 dpy-20(e1282) unc-22(e66) IV. C. elegans Dpy. Twitcher.
KK26 unc-4(e120) ooc-3(b310)/mnC1 [dpy-10(e128) unc-52(e444)] II. C. elegans Heterozygotes are WT and segregate WT, DpyUnc and Unc-4 which give dead eggs. Maintain by picking WT. Strict maternal effect.
KK28 unc-4(e120) mel-17(b299)/mnC1 [dpy-10(e128) unc-52(e444)] II. C. elegans Heterozygotes are WT and segregate WT, DpyUnc and Unc-4s which give only dead eggs. Maintain by picking WT. Strict maternal effect.
KK288 sqt-3(sc8) par-1(b274) IV/nT1 [unc-?(n754) let-?] (IV;V). C. elegans Heterozygotes are Unc and segregate Unc, RolPar (adult homozygotes lay eggs that don't hatch) and dead eggs. nT1[unc-?(n754) let-?] is dominant Unc and recessive lethal. Strict maternal effect. sc8 previously called rol-4(sc8).
KK299 par-5(it55) unc-22(e66) IV/nT1 [unc-?(n754) let-?] (IV;V). C. elegans Heterozygotes are Unc and segregate Unc, Twitchers which give only dead eggs, and dead eggs. nT1 heterozygotes are shorter and slower than par-5 unc-22 homozygous worms. par-5 region is not well balanced by nT1: check to make sure that unc-22 homozygotes lay dead eggs. Strict maternal effect lethal.
KK300 par-4(it57) V. C. elegans Temperature-sensitive maternal effect lethal (strict). Maintain at 15-16C (95% of embyros hatch). Shift to 25C for Par phenotype (no hatching embryos).
KK312 him-14(it44) II. C. elegans Maternal effect lethal mutation. High incidence of males in surviving offspring. Not rescued by male mating. Temperature sensitive.
KK332 mel-11(it26) unc-4(e120) sqt-1(sc13)/mnC1 [dpy-10(e128) unc-52(e444)] II. C. elegans Heterozygotes are WT and segregate WT, DpyUnc, and UncRol (which give only dead eggs). Maintain by picking WT. mel-11 is not strict. See also WBPaper00002726.
KK337 unc-4(e120) mex-1(it9)/mnC1 [dpy-10(e128) unc-52(e444)] II. C. elegans Heterozygotes are WT and segregate WT, DpyUnc and Unc-4 which give dead eggs. Maintain by picking WT. Strict maternal effect. it9 was previously called mel-21(it9).
KK338 mel-10(it10) unc-4(e120) sqt-1(sc13)/mnC1 [dpy-10(e128) unc-52(e444)] II. C. elegans Heterozygotes are WT and segregate WT, DpyUnc and Unc-4s which give only dead eggs. Maintain by picking WT. Strict maternal effect.
KK34 unc-4(e120) mel-16(b298)/mnC1 [dpy-10(e128) unc-52(e444)] II. C. elegans Heterozygotes are WT and segregate WT, DpyUnc and Unc-4s which give only dead eggs. Maintain by picking WT.
KK344 unc-4(e120) mel-22(it30)/mnC1 [dpy-10(e128) unc-52(e444)] II. C. elegans Heterozygotes are WT and segregate WT, DpyUnc and Unc-4 which give dead eggs. Maintain by picking WT. Strict maternal effect.
KK352 unc-4(e120) top-2(it38)/mnC1 [dpy-10(e128) unc-52(e444)] II. C. elegans Heterozygotes are WT and segregate WT, DpyUnc and Unc-4s which give only dead eggs. Maintain by picking WT. Not a strict maternal effect-rescued by male mating.
KK356 mel-4(it12) unc-4(e120)/mnC1 [dpy-10(e128) unc-52(e444)] II. C. elegans Heterozygotes are WT and segregate WT, DpyUnc and Unc-4s which give only dead eggs (it12 is temperature sensitive). Maintain by picking WT. Strict maternal effect.
KK357 mel-1(it19) unc-4(e120)/mnC1 [dpy-10(e128) unc-52(e444)] II. C. elegans Heterozygotes are WT and segregate WT, DpyUnc and Unc-4s which give only dead eggs. Maintain by picking WT. Strict maternal effect.
KK359 tofu-6(it20) unc-4(e120)/mnC1 [dpy-10(e128) unc-52(e444)] II. C. elegans Heterozygotes are WT and segregate WT, DpyUnc and Unc-4s which give only dead eggs. Maintain by picking WT. Strict maternal effect.
KK36 unc-4(e120) mel-13(b306) sqt-1(sc13)/mnC1 [dpy-10(e128) unc-52(e444)] II. C. elegans Heterozygotes are WT and segregate WT, paralysed DpyUnc and RolUnc-4 which give dead eggs. Maintain by picking WT. Strict maternal effect.
KK364 mel-8(it27) unc-4(e120)/mnC1 [dpy-10(e128) unc-52(e444)] II. C. elegans mel-8 is a maternal effect lethal. WT heterozygotes segregate WT; mel-8 unc-4 homozygotes that lay eggs that don't hatch, and mnC1 dpy-10 unc-52 homozygotes (paralyzed Dpys). Some F2 mel homozygotes hatch, but such escapers arrest at L1 or L2. Previously called mel-6(it27).
KK366 mel-7(it36) unc-4(e120)/mnC1 [dpy-10(e128) unc-52(e444)] II. C. elegans Heterozygotes are WT and segregate WT, DpyUnc and Unc-4s which give only dead eggs. Maintain by picking WT. Strict maternal effect.
KK37 mel-5(b314) unc-4(e120)/mnC1 [dpy-10(e128) unc-52(e444)] II. C. elegans Heterozygotes are WT and segregate WT, DpyUnc and Unc-4s which give only dead eggs (0.5% leaky). Maintain by picking WT. Not a strict maternal effect.
KK375 mel-12(it42) unc-4(e120)/mnC1 [dpy-10(e128) unc-52(e444)] II. C. elegans Heterozygotes are WT and segregate WT, DpyUnc and Unc-4s which give only dead eggs. Maintain by picking WT. Not a strict maternal effect-rescued by male mating.
KK40 mel-3(b281) unc-4(e120)/mnC1 [dpy-10(e128) unc-52(e444)] II. C. elegans Heterozygotes are WT and segregate WT, DpyUnc and Unc-4s which give only dead eggs. Maintain by picking WT. Strict maternal effect.
KK41 unc-4(e120) mel-20(b317)/mnC1 [dpy-10(e128) unc-52(e444)] II. C. elegans Heterozygotes are WT and segregate WT, DpyUnc and Unc-4s which give only dead eggs. Maintain by picking WT. Not a strict maternal effect-rescued by male mating.
KK436 unc-4(e120) mel-14(it24)/mnC1 [dpy-10(e128) unc-52(e444)] II. C. elegans Heterozygotes are WT and segregate WT, DpyUnc and Unc-4s which give only dead eggs (approx .2% leaky). Maintain by picking WT.
KK49 unc-4(e120) mel-18(b300)/mnC1 [dpy-10(e128) unc-52(e444)] II. C. elegans Heterozygotes are WT and segregate WT, DpyUnc and Unc-4s which give only dead eggs. Maintain by picking WT. Not a strict maternal effect-rescued by male mating.
KK571 lon-1(e185) par-3(it71)/qC1 [dpy-19(e1259) glp-1(q339)] III. C. elegans Heterozygotes are WT and segregate WT, Dpy Steriles, and Lon which give only dead eggs.
KK627 itDf2 V/nT1 [unc-?(n754) let-?] (IV;V). C. elegans Heterozygotes are Unc and segregate Uncs and dead eggs.
KK725 nop-1(it142) III. C. elegans Recessive maternal effect. Embryos from nop-1 mothers exhibit no pseudocleavage contractions or furrow. The majority of the embryos survive and grow into fertile adults.
KK818 par-6(zu222) unc-101(m1)/hIn1 [unc-54(h1040)] I. C. elegans Heterozygotes are WT and segregate WT, paralyzed Unc, and Coilers which give only dead eggs (slightly leaky, but survivors are agametic). zu222 is a strict maternal effect embryonic lethal. Partitioning defect similar to that of par-3; fails to localize PAR-3 protein.
KK822 par-1(zu310) V. C. elegans Temperature-sensitive maternal effect lethal. Maintain at 15C. At 25C embryos show polarity defects and fail to hatch. Reference: Morton DG, Hoose WA, Kemphues KJ. Genetics. 2012 Aug 10.

Alleles contributed by this laboratory

Allele Type DNA Change Protein Change
it21 Allele substitution nonsense
it132 Allele substitution
it127 Allele substitution
it112 Allele
it118 Allele
it110 Allele substitution splice_site
it33 Allele substitution
it143 Allele substitution
it71 Allele
it46 Allele
it5 Allele
it47 Allele substitution
it62 Allele
it55 Allele substitution
it57 Allele substitution
it44 Allele substitution
it26 Allele
it9 Allele
it10 Allele
it30 Allele
it38 Allele
it12 Allele
it19 Allele
it20 Allele substitution nonsense
it27 Allele
it36 Allele
it42 Allele
it24 Allele
it145 Allele substitution nonsense
it142 Allele substitution nonsense
it25 Allele substitution
it149 Allele
it150 Allele substitution splice_site
it129 Allele substitution