KK112 |
mel-8(b309) unc-4(e120) sqt-1(sc13)/mnC1 [dpy-10(e128) unc-52(e444)] II. |
C. elegans |
mel-8 is a maternal effect lethal. WT heterozygotes (sc13 recessive) segregate WT, MelUncSqt (sick and small), mnC1 dpy-10 unc-52 homozygotes (paralyzed Dpys). Rescued by male mating. |
KK114 |
daf-7(e1372) par-2(it5) III. |
C. elegans |
Temperature sensitive-grow at 15C. Par at 25C. Strict maternal effect lethal. |
KK1216 |
par-3(it298[par-3::GFP]) III. |
C. elegans |
Superficially wild-type. Made in N2 background. |
KK1218 |
par-3(it300[par-3::mCherry]) III. |
C. elegans |
Superficially wild-type. Made in N2 background. |
KK1228 |
pkc-3(it309[GFP::pkc-3]) II. |
C. elegans |
Superficially wild-type. Note that double homozygous mutants of pkc-3(it309) with par-2(it315) exhibited partially penetrant and variable maternal effect lethality and maternal effect sterility that was stronger than it315 alone. Thus, although the GFP tag on pkc-3 shows no effect in an otherwise wild-type background, it does seem to somewhat compromise the activity of the protein it tags. Made in N2 background. |
KK1248 |
par-6(it319[par-6::GFP]) I. |
C. elegans |
Superficially wild-type. Made in N2 background. |
KK1254 |
par-2(it315[mCherry::par-2]) III. |
C. elegans |
par-2(it315) exhibits a weak maternal effect sterility, suggesting that the tag reduces the protein activity. Note, however, that double homozygous mutants of it315 with pkc-3(it309) or par-6(it310) exhibited partially penetrant and variable maternal effect lethality and maternal effect sterility that was stronger than it315 alone. Thus, although the GFP tags on pkc-3 and par-6 show no effect in an otherwise wild-type background, they do seem to somewhat compromise the activity of the proteins they tag. Made in N2 background. |
KK1262 |
par-1(it324[par-1::GFP::par-1 exon11a]) V. |
C. elegans |
Superficially wild-type. Made in N2 background. |
KK1273 |
par-2(it328[GFP::par-2]) III. |
C. elegans |
Superficially wild-type. Made in N2 background. |
KK157 |
mel-9(b293) II. |
C. elegans |
Maternal effect lethal mutation. Temperature sensitive-grow at 15C. Some growth at 20C. Does not grow at 25C. Rescued by male mating. |
KK184 |
par-4(it47) V. |
C. elegans |
Strict maternal effect lethal. Temperature sensitive-grow at 15C. Par at 20C and 25C. |
KK204 |
sma-4(e729) mab-5(e1239) unc-36(e251) III. |
C. elegans |
Small and Unc. |
KK237 |
lon-1(e185) par-3(e2074) III; sDp3 (III;f). |
C. elegans |
WT sDp3-bearing animals segregate WT and lon-1 par-3 homozygotes that lay eggs that don't hatch. par-3 exhibits mildly ts penetrance in homozygotes. Strict maternal effect. Received new stock 3/01. |
KK241 |
lon-1(e185) par-3(it62) III; sDp3 (III;f). |
C. elegans |
Animals with the Duplication are WT. Animals which have lost the Duplication are Lon and throw dead eggs. |
KK255 |
dpy-20(e1282) unc-22(e66) IV. |
C. elegans |
Dpy. Twitcher. |
KK26 |
unc-4(e120) ooc-3(b310)/mnC1 [dpy-10(e128) unc-52(e444)] II. |
C. elegans |
Heterozygotes are WT and segregate WT, DpyUnc and Unc-4 which give dead eggs. Maintain by picking WT. Strict maternal effect. |
KK28 |
unc-4(e120) mel-17(b299)/mnC1 [dpy-10(e128) unc-52(e444)] II. |
C. elegans |
Heterozygotes are WT and segregate WT, DpyUnc and Unc-4s which give only dead eggs. Maintain by picking WT. Strict maternal effect. |
KK288 |
sqt-3(sc8) par-1(b274) IV/nT1 [unc-?(n754) let-?] (IV;V). |
C. elegans |
Heterozygotes are Unc and segregate Unc, RolPar (adult homozygotes lay eggs that don't hatch) and dead eggs. nT1[unc-?(n754) let-?] is dominant Unc and recessive lethal. Strict maternal effect. sc8 previously called rol-4(sc8). |
KK299 |
par-5(it55) unc-22(e66) IV/nT1 [unc-?(n754) let-?] (IV;V). |
C. elegans |
Heterozygotes are Unc and segregate Unc, Twitchers which give only dead eggs, and dead eggs. nT1 heterozygotes are shorter and slower than par-5 unc-22 homozygous worms. par-5 region is not well balanced by nT1: check to make sure that unc-22 homozygotes lay dead eggs. Strict maternal effect lethal. |
KK300 |
par-4(it57) V. |
C. elegans |
Temperature-sensitive maternal effect lethal (strict). Maintain at 15-16C (95% of embyros hatch). Shift to 25C for Par phenotype (no hatching embryos). |
KK312 |
him-14(it44) II. |
C. elegans |
Maternal effect lethal mutation. High incidence of males in surviving offspring. Not rescued by male mating. Temperature sensitive. |
KK332 |
mel-11(it26) unc-4(e120) sqt-1(sc13)/mnC1 [dpy-10(e128) unc-52(e444)] II. |
C. elegans |
Heterozygotes are WT and segregate WT, DpyUnc, and UncRol (which give only dead eggs). Maintain by picking WT. mel-11 is not strict. See also WBPaper00002726. |
KK337 |
unc-4(e120) mex-1(it9)/mnC1 [dpy-10(e128) unc-52(e444)] II. |
C. elegans |
Heterozygotes are WT and segregate WT, DpyUnc and Unc-4 which give dead eggs. Maintain by picking WT. Strict maternal effect. it9 was previously called mel-21(it9). |
KK338 |
mel-10(it10) unc-4(e120) sqt-1(sc13)/mnC1 [dpy-10(e128) unc-52(e444)] II. |
C. elegans |
Heterozygotes are WT and segregate WT, DpyUnc and Unc-4s which give only dead eggs. Maintain by picking WT. Strict maternal effect. |
KK34 |
unc-4(e120) mel-16(b298)/mnC1 [dpy-10(e128) unc-52(e444)] II. |
C. elegans |
Heterozygotes are WT and segregate WT, DpyUnc and Unc-4s which give only dead eggs. Maintain by picking WT. |
KK344 |
unc-4(e120) mel-22(it30)/mnC1 [dpy-10(e128) unc-52(e444)] II. |
C. elegans |
Heterozygotes are WT and segregate WT, DpyUnc and Unc-4 which give dead eggs. Maintain by picking WT. Strict maternal effect. |
KK352 |
unc-4(e120) top-2(it38)/mnC1 [dpy-10(e128) unc-52(e444)] II. |
C. elegans |
Heterozygotes are WT and segregate WT, DpyUnc and Unc-4s which give only dead eggs. Maintain by picking WT. Not a strict maternal effect-rescued by male mating. |
KK356 |
mel-4(it12) unc-4(e120)/mnC1 [dpy-10(e128) unc-52(e444)] II. |
C. elegans |
Heterozygotes are WT and segregate WT, DpyUnc and Unc-4s which give only dead eggs (it12 is temperature sensitive). Maintain by picking WT. Strict maternal effect. |
KK357 |
mel-1(it19) unc-4(e120)/mnC1 [dpy-10(e128) unc-52(e444)] II. |
C. elegans |
Heterozygotes are WT and segregate WT, DpyUnc and Unc-4s which give only dead eggs. Maintain by picking WT. Strict maternal effect. |
KK359 |
tofu-6(it20) unc-4(e120)/mnC1 [dpy-10(e128) unc-52(e444)] II. |
C. elegans |
Heterozygotes are WT and segregate WT, DpyUnc and Unc-4s which give only dead eggs. Maintain by picking WT. Strict maternal effect. |
KK36 |
unc-4(e120) mel-13(b306) sqt-1(sc13)/mnC1 [dpy-10(e128) unc-52(e444)] II. |
C. elegans |
Heterozygotes are WT and segregate WT, paralysed DpyUnc and RolUnc-4 which give dead eggs. Maintain by picking WT. Strict maternal effect. |
KK364 |
mel-8(it27) unc-4(e120)/mnC1 [dpy-10(e128) unc-52(e444)] II. |
C. elegans |
mel-8 is a maternal effect lethal. WT heterozygotes segregate WT; mel-8 unc-4 homozygotes that lay eggs that don't hatch, and mnC1 dpy-10 unc-52 homozygotes (paralyzed Dpys). Some F2 mel homozygotes hatch, but such escapers arrest at L1 or L2. Previously called mel-6(it27). |
KK366 |
mel-7(it36) unc-4(e120)/mnC1 [dpy-10(e128) unc-52(e444)] II. |
C. elegans |
Heterozygotes are WT and segregate WT, DpyUnc and Unc-4s which give only dead eggs. Maintain by picking WT. Strict maternal effect. |
KK37 |
mel-5(b314) unc-4(e120)/mnC1 [dpy-10(e128) unc-52(e444)] II. |
C. elegans |
Heterozygotes are WT and segregate WT, DpyUnc and Unc-4s which give only dead eggs (0.5% leaky). Maintain by picking WT. Not a strict maternal effect. |
KK375 |
mel-12(it42) unc-4(e120)/mnC1 [dpy-10(e128) unc-52(e444)] II. |
C. elegans |
Heterozygotes are WT and segregate WT, DpyUnc and Unc-4s which give only dead eggs. Maintain by picking WT. Not a strict maternal effect-rescued by male mating. |
KK40 |
mel-3(b281) unc-4(e120)/mnC1 [dpy-10(e128) unc-52(e444)] II. |
C. elegans |
Heterozygotes are WT and segregate WT, DpyUnc and Unc-4s which give only dead eggs. Maintain by picking WT. Strict maternal effect. |
KK41 |
unc-4(e120) mel-20(b317)/mnC1 [dpy-10(e128) unc-52(e444)] II. |
C. elegans |
Heterozygotes are WT and segregate WT, DpyUnc and Unc-4s which give only dead eggs. Maintain by picking WT. Not a strict maternal effect-rescued by male mating. |
KK436 |
unc-4(e120) mel-14(it24)/mnC1 [dpy-10(e128) unc-52(e444)] II. |
C. elegans |
Heterozygotes are WT and segregate WT, DpyUnc and Unc-4s which give only dead eggs (approx .2% leaky). Maintain by picking WT. |
KK49 |
unc-4(e120) mel-18(b300)/mnC1 [dpy-10(e128) unc-52(e444)] II. |
C. elegans |
Heterozygotes are WT and segregate WT, DpyUnc and Unc-4s which give only dead eggs. Maintain by picking WT. Not a strict maternal effect-rescued by male mating. |
KK571 |
lon-1(e185) par-3(it71)/qC1 [dpy-19(e1259) glp-1(q339)] III. |
C. elegans |
Heterozygotes are WT and segregate WT, Dpy Steriles, and Lon which give only dead eggs. |
KK627 |
itDf2 V/nT1 [unc-?(n754) let-?] (IV;V). |
C. elegans |
Heterozygotes are Unc and segregate Uncs and dead eggs. |
KK725 |
nop-1(it142) III. |
C. elegans |
Recessive maternal effect. Embryos from nop-1 mothers exhibit no pseudocleavage contractions or furrow. The majority of the embryos survive and grow into fertile adults. |
KK818 |
par-6(zu222) unc-101(m1)/hIn1 [unc-54(h1040)] I. |
C. elegans |
Heterozygotes are WT and segregate WT, paralyzed Unc, and Coilers which give only dead eggs (slightly leaky, but survivors are agametic). zu222 is a strict maternal effect embryonic lethal. Partitioning defect similar to that of par-3; fails to localize PAR-3 protein. |
KK822 |
par-1(zu310) V. |
C. elegans |
Temperature-sensitive maternal effect lethal. Maintain at 15C. At 25C embryos show polarity defects and fail to hatch. Reference: Morton DG, Hoose WA, Kemphues KJ. Genetics. 2012 Aug 10. |