Laboratory Information

NameJCP View on WormBase
Allele designationjcp
HeadCabello, Juan
InstitutionCenter for Biomedical Research of La Rioja, Logrono, Spain
Address Fundacion Rioja Salud - CIBIR
C/Pîqueras 98, 2º planta
Logroño 26006
Spain
Website http://www.riojasalud.es/cibir-personal/2074-juan-cabello
Gene classes kdin 

Strains contributed by this laboratory

Strain Genotype Species Description
GE2001 xpf-1(e1487) II; unc-24(e138) IV/nT1(IV;V); dpy-11(e224) ccz-1(t2070) V/nT1 (IV;V). C. elegans Him. Wild-type heterozygotes segregate WT heterozygotes and Dpy Unc ccz-1 homozygotes (produce only arrested embryos). ccz-1 embryos have spindle orientation defects, accumulate vesicles, and problems engulfing apoptotic corpses. Reference: Nieto C, et al. J Cell Sci. 2010 Jun 15;123(Pt 12):2001-7.
GE2516 xpf-1(e1487) II; unc-24(e138) IV/nT1(IV;V); dpy-11(e224) ccz-1(t2071) V/nT1 (IV;V). C. elegans Him. Wild-type heterozygotes segregate WT heterozygotes and Dpy Unc ccz-1 homozygotes (produce only arrested embryos). ccz-1 embryos have spindle orientation defects, accumulate vesicles, and problems engulfing apoptotic corpses. Reference: Nieto C, et al. J Cell Sci. 2010 Jun 15;123(Pt 12):2001-7.
JCP152 dpy-11(e224) ccz-1(t2129) V; jcpEx2. C. elegans jcpEx2 [ced-1p::F58G11.6(genomic)::YFP::let-858 3'UTR + unc-119(+) + myo-2::GFP]. Maintain by picking GFP+. Individuals that lost the array produce only arrested embryos with spindle orientation defects, accumulate vesicles, and problems engulfing apoptotic corpses. Reference: Nieto C, et al. J Cell Sci. 2010 Jun 15;123(Pt 12):2001-7.
JCP169 dpy-11(e224) ccz-1(t2129) V; jcpEx3. C. elegans jcpEx3 [ccz-1p::ccz-1(genomic)::YFP::let-858 3'UTR + unc-119(+) + pha-1(+)]. Array rescues lethality. Individuals that lost the array produce only arrested embryos with spindle orientation defects, accumulate vesicles, and problems engulfing apoptotic corpses. Reference: Nieto C, et al. J Cell Sci. 2010 Jun 15;123(Pt 12):2001-7.
JCP294 ints-6(t1903) IV. C. elegans Temperature-sensitive. Grows well at 15C; embryonic lethal at 25C. ints-6 previously known as dic-1. Reference: Gómez-Orte E, et al. PLoS Genet. 2019 Feb 26;15(2):e1007981.
JCP301 jcpSi3 II; unc-119(ed3) III. C. elegans jcpSi3 [ins-37p::ins-37::eGFP::ins-37 3'UTR + unc-119(+)] II. Superficially wild-type. Reference: Gómez-Orte E, et al. PLoS Genet. 2019 Feb 26;15(2):e1007981.
JCP341 jcpSi10 II; unc-119(ed3) III. C.elegans jcpSi10 [ints-6p::ints-6::3xFLAG::eGFP::ints-6 3'UTR + unc-119(+)] II. Superficially wild-type. ints-6 previously known as dic-1. Reference: Gómez-Orte E, et al. PLoS Genet. 2019 Feb 26;15(2):e1007981.
JCP343 jcpSi12 II; unc-119(ed3) III. C. elegans jcpSi12 [W04G5.8p::W04G5.8::eGFP::W04G5.8 3'UTR + unc-119(+)] II. Superficially wild-type. Reference: Gómez-Orte E, et al. PLoS Genet. 2019 Feb 26;15(2):e1007981.
JCP378 jcpSi19 II; unc-119(ed3) III. C. elegans jcpSi19 [eft-3p::ints-6::3xFLAG::eGFP::ints-6 3'UTR + unc-119(+)] II. Superficially wild-type. ints-6 previously known as dic-1. Reference: Gómez-Orte E, et al. PLoS Genet. 2019 Feb 26;15(2):e1007981.
JCP383 jcpSi10 II; ints-6(tm1615) IV. C. elegans jcpSi10 [ints-6p::ints-6::3xFLAG::eGFP::ints-6 3'UTR + unc-119(+)] II. Superficially wild-type. ints-6 previously known as dic-1. Reference: Gómez-Orte E, et al. PLoS Genet. 2019 Feb 26;15(2):e1007981.
JCP387 jcpSi24 II; unc-119(ed3) III. C. elegans jcpSi24 [H27M09.5p::H27M09.5::3xFLAG::eGFP::H27M09.5 3'UTR + unc-119(+)] II. Superficially wild-type. Reference: Gómez-Orte E, et al. PLoS Genet. 2019 Feb 26;15(2):e1007981.
JCP394 jcpSi31 II; unc-119(ed3) III. C. elegans jcpSi31 [Y75B8A.23p::Y75B8A.23::3xFLAG::eGFP::Y75B8A.2 3'UTR + unc-119(+)] II. Superficially wild-type. Reference: Gómez-Orte E, et al. PLoS Genet. 2019 Feb 26;15(2):e1007981.
JCP405 jcpSi37 II; unc-119(ed3) III. C. elegans jcpSi37 [F08H9.3p::F08H9.3::3xFLAG::eGFP::F08H9.3 3'UTR + unc-119(+)] II. Superficially wild-type. Reference: Gómez-Orte E, et al. PLoS Genet. 2019 Feb 26;15(2):e1007981.
JCP462 ints-6(jcp1[ints-6::3xFLAG]) IV. C. elegans 3xFLAG tag inserted into the endogenous ints-6 locus. Superficially wild-type. ints-6 previously known as dic-1. Reference: Gómez-Orte E, et al. PLoS Genet. 2019 Feb 26;15(2):e1007981.
JCP484 nxf-1(t2160) V; pxIs10. C. elegans pxIs10 [pha-4::GFP::CAAX + rol-6(su1006)]. Temperature-sensitive. Maintain at 15-20C. 100% embryonic lethal at 25C. Pharynx unattached (Pun) and morphogenic defects. Roller line that has GFP localized to the plasma membrane of the pharynx, gut and rectal cells in embryos and the somatic gonad during L2-L3 larval stage and beyond. Reference: Zheleva A, et al. PLoS Genet. 2019 Sep 16;15(9):e1008338.
JCP485 nxf-1(t2160) V; jcIs17 [hmp-1p::hmp-1::GFP + dlg-1p::dlg-1::dsRed + rol-6(su1006)] C. elegans Temperature sensitive
JCP486 nxf-1(t2160) V; xnIs96 [pJN455(hmr-1p::hmr-1::GFP::unc-54 3'UTR) + unc-119(+)] C. elegans Temperature sensitive
JCP489 nxf-1(t2160) V; jcIs25 [pPE103 (jac-1::GFP) + rol-6(su1006)] C. elegans Temperature sensitive.
JCP495 nxf-1(t2160) V. C. elegans Temperature-sensitive. Maintain at 15-20C. 100% embryonic lethal at 25C. Pharynx unattached (Pun) and morphogenic defects. Reference: Zheleva A, et al. PLoS Genet. 2019 Sep 16;15(9):e1008338.
JCP506 nxf-1(t2160) V; ddIs290 [sax-7::TY1::EGFP::3xFLAG(92C12) + unc-119(+)] C. elegans Temperature sensitive
JCP510 nxf-1(t2160) V; syls50[cdh-3::GFP + dpy-20(+)] C. elegans Temperature sensitive
JCP511 nxf-1(t2160) V; mcIs50 [lin-26p::vab-10(actin-binding domain)::GFP + myo-2p::GFP + pBluescript] C. elegans Temperature sensitive
JCP519 nxf-1(t2160) V; jcpEx6. C. elegans jcpEx6 [nxf-1p::nxf-1::3xFLAG::eGFP::nxf-1UTR]. jcpEx6 transgene rescues nxf-1(t2160) embryonic lethality at 25C. Temperature-sensitive; maintain at 25C to retain the array. nxf-1(t2160) mutants are 100% embryonic lethal at 25C. Reference: Zheleva A, et al. PLoS Genet. 2019 Sep 16;15(9):e1008338.
JCP53 dpy-11(e224) ccz-1(t2129) V/nT1 [qIs51] (IV;V). C. elegans Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested nT1[qIs51] aneuploids, and non-GFP ccz-1 homozygotes (produce only arrested embryos with spindle orientation defects, accumulate vesicles, and problems engulfing apoptotic corpses). Homozygous nT1[qIs51] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. Reference: Nieto C, et al. J Cell Sci. 2010 Jun 15;123(Pt 12):2001-7.
JCP543 nxf-1(t2160) V; ncIs13[ajm-1::GFP] C. elegans Temperature sensitive.
JCP561 unc-119(ed3) III; nxf-1(t2160) V; wgIs37. C. elegans wgIs37 [pha-4::TY1::EGFP::3xFLAG + unc-119(+)]. Temperature-sensitive. Maintain at 15-20C. 100% embryonic lethal at 25C. Pharynx unattached (Pun) and morphogenic defects. Reference: Zheleva A, et al. PLoS Genet. 2019 Sep 16;15(9):e1008338.
JCP565 nxf-1(t2160) V; ccIs4251 [(pSAK2) myo-3p::GFP::LacZ::NLS + (pSAK4) myo-3p::mitochondrial GFP + dpy-20(+)] C. elegans Temperature sensitive
JCP567 etIs1 IV; nxf-1(t2160) V. C. elegans etIs1 [ric-19p::ric-19::GFP + rol-6(su1006)] IV. Temperature-sensitive. Maintain at 15-20C. 100% embryonic lethal at 25C. Pharynx unattached (Pun) and morphogenic defects. Reference: Zheleva A, et al. PLoS Genet. 2019 Sep 16;15(9):e1008338.
JCP633 unc-119(ed3) III; nxf-1(t2160) V; xnIs312 [par-6p::par-6::mCherry + unc-119(+)] C. elegans Temperature sensitive
WS5235 ccz-1(t2129) V/nT1 [qIs51] (IV;V). C. elegans Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested nT1[qIs51] aneuploids, and non-GFP ccz-1 homozygotes (produce only arrested embryos with spindle orientation defects, accumulate vesicles, and problems engulfing apoptotic corpses). Homozygous nT1[qIs51] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. Reference: Nieto C, et al. J Cell Sci. 2010 Jun 15;123(Pt 12):2001-7.
This laboratory hasn't submitted any alleles to the CGC.