Laboratory Information

NameEW View on WormBase
Allele designationde
HeadEisenmann, David
InstitutionUMBC, Baltimore, MD
Address Dave Eisenmann
UMBC Biological Sciences
1000 Hilltop Circle
Baltimore 21250
United States
Website http://www.umbc.edu/biosci/Faculty/eisenmann.html
Gene classes

Strains contributed by this laboratory

Strain Genotype Species Description
EW10 pvl-5(ga87) II. C. elegans Egl. Low penetrance embryonic lethal.
EW12 mig-14(ga62) II. C. elegans Egl. Low penetrance embryonic lethal. mig-14, mom-3, pvl-2, and let-553 are the same gene.
EW15 bar-1(ga80) X. C. elegans [NOTE: (10/22/2020) This strain also carries a (T to A) missense mutation in pry-1 which results in a PRY-1 N354K amino acid substitution.] bar-1(ga80) hermaphrodites are usually Egl and often have a protruding vulva (Pvl), although approx. 40% of animals appear WT on plates. Also slightly Unc. In bar-1(ga80) hermaphrodites any of the six vulval precursor cells (P3.p - P8.p) can sometimes fuse with hyp7 without dividing, and P5.p - P7.p can adopt the tertiary cell fate instead of the primary or secondary fates. In addition, the neuroblast QL and its progeny migrate towards the anterior instead of the posterior, and the cell P12 usually adopts the fate of P11. bar-1(ga80) do mate, but poorly. bar-1 encodes a beta-catenin molecule and the ga80 mutation is predicted to cause an early truncation of the protein.
EW23 unc-104(e1265) rol-6(e187) II. C. elegans Dpy. Roller.
EW32 lin-26(ga91) II. C. elegans Pvl. Egl.
EW33 unc-4(e120) pvl-4(ga96) II. C. elegans Egl. Pvl. Misshapen larvae (some lethal). Unc.
EW34 egl-18(ga97) IV. C. elegans Egl. Rol.
EW35 sem-4(ga82) I. C. elegans Egl.
EW45 smg-1(e1228) I; unc-30(e191) IV; deIs1. C. elegans deIs1[unc-30(+) + lin-39TL::GFP (yeast DNA)]. A translational fusion of GFP to the C terminus of LIN-39. Present on a YAC. Yeast chromosomal DNA was injected and integrated. Expression is very faint.
EW49 unc-30(e191) IV; deIs12. C. elegans deIs12[bar1p::GFP + pSC11(unc-30(+))]. Green worms that are non-Unc.
EW5 pvl-6(ga81) V. C. elegans Egl.
EW51 pvl-5(de4) II. C. elegans de4 is a weaker allele of pvl-5. Weakly penetrant Egl and Pvl phenotype. Low penetrance embryonic lethal and gonad migration defects. pvl-5(de4) animals have fewer Pn.p cells in the ventral midline at mid-L2 larval stage.
EW52 lin-44(n1792) I; cwn-2(ok895) IV. C. elegans
EW53 lin-44(n1792) I; cwn-1(ok546) II; cwn-2(ok895) IV. C. elegans
EW55 dpy-20(e1362) IV; deEx100. C. elegans deEx100 [ajm-1::GFP + HinfI digested complex DNA + lin-44p::4xNLS::GFP + dpy20(+)]. Pick non-Dpy to maintain.
EW56 dpy-20(e1362) IV; deEx101. C. elegans deEx101[dpy20(+); ajm-1::GFP; HinfI digested complex DNA; cwn-1p::4xNLSGFP]. Pick non-Dpy to maintain.
EW57 dpy-20(e1362) IV; deEx102. C. elegans deEx102[dpy20(+); ajm-1::GFP; HinfI digested complex DNA; egl-20p::4xNLSGFP]. Pick non-Dpy to maintain.
EW58 dpy-20(e1362) IV; deEx103. C. elegans deEx103[dpy20(+); ajm-1::GFP; HinfI digested complex DNA; cwn-2p::4xNLSGFP]. Pick non-Dpy to maintain.
EW59 dpy-20(e1362) IV; deEx104. C. elegans deEx104 [ajm-1::GFP + mom-2p::4xNLS::GFP + dpy20(+) + HinfI digested complex DNA]. Pick non-Dpy to maintain.
EW61 dpy-20(e1282) IV; him-5(e1490) V; deIs4. C. elegans deIs4 [ajm-1::GFP + GFP::lin-39(YAC) + dpy-20(+)]. A transcriptional fusion of GFP to the ATG of lin-39, with a stop codon between GFP and LIN-39 sequences. Present on a YAC. Yeast chromosomal DNA was injected and integrated. Expression is very faint and may get fainter over time. Freeze upon receipt.
EW62 smg-1(e1228) I; dpy-20(e1282) IV; him-5(e1490) V; deIs4. C. elegans deIs4 [ajm-1::GFP + GFP::lin-39(YAC) + dpy-20(+)]. A transcriptional fusion of GFP to the ATG of lin-39, with a stop codon between GFP and LIN-39 sequences. Present on a YAC. Yeast chromosomal DNA was injected and integrated. Expression is very faint and may get fainter over time. Therefore, freeze upon receipt.
EW68 lin-44(n1792) I; cwn-1(ok546) II. C. elegans
EW72 cwn-1(ok546) II; cwn-2(ok895) IV. C. elegans
EW75 cwn-2(ok895) IV/nT1 [qIs51] (IV;V); dpy-11(e1180) mom-2(or42) V/nT1 [qIs51] (IV;V). C. elegans Heterozygotes are WT and GFP+. Segregate more WT and GFP+, Dpys, and dead eggs. Dpys give only embryonic lethals.
EW77 lin-44(n1792) I; egl-20(n585) IV/nT1 [qIs51] (IV;V); dpy-11(e1180) mom-2(or42) V/nT1 [qIs51] (IV;V). C. elegans
EW79 cwn-1(ok546) II; egl-20(n585) IV/nT1 [qIs51] (IV;V); dpy-11(e1180) mom-2(or42) V/nT1 [qIs51] (IV;V). C. elegans Heterozygotes are WT and GFP+. Segregate more WT and GFP+, Dpys, and dead eggs. Dpys give only embryonic lethals.
EW80 cwn-1(ok546) II; cwn-2(ok895) IV/nT1[qIs51] (IV;V); dpy-11(e1180) mom-2(or42) V/nT1[qIs51] (IV;V). C. elegans Heterozygotes are WT and GFP+. Segregate more WT and GFP+, Dpys, and dead eggs. Dpys give only embryonic lethals.
KN594 cwn-1(ok546) II; egl-20(n585) IV. C. elegans
KS46 lin-44(n1792) I; egl-20(n585) IV. C. elegans
SD551 let-60(ga89) IV. C. elegans Temperature sensitive. Nearly WT at 15C. At 20C the animals are 18% Muv and brood size is 88. At 25C the animals are 57% Muv and almost sterile (brood size is 6). Maintain at 15C.
temp_name262 cwn-1(ok546) II; cwn-2(ok895) IV/nT1 [qIs51] (IV;V); dpy-11(e1180) mom-2(or42) V/nT1 [qIs51] (IV;V). Balancer has broken down; no replacement available from EW

Alleles contributed by this laboratory

Allele Type DNA Change Protein Change
de4 Allele