Laboratory Information
| Name | EW View on WormBase |
|---|---|
| Allele designation | de |
| Head | Eisenmann, David |
| Institution | UMBC, Baltimore, MD |
| Address | Dave Eisenmann UMBC Biological Sciences 1000 Hilltop Circle Baltimore 21250 United States |
| Website | http://www.umbc.edu/biosci/Faculty/eisenmann.html |
| Gene classes |
Strains contributed by this laboratory
| Strain | Genotype | Species | Description |
|---|---|---|---|
| EW10 | pvl-5(ga87) II. | C. elegans | Egl. Low penetrance embryonic lethal. |
| EW12 | mig-14(ga62) II. | C. elegans | Egl. Low penetrance embryonic lethal. mig-14, mom-3, pvl-2, and let-553 are the same gene. |
| EW15 | bar-1(ga80) X. | C. elegans | [NOTE: (10/22/2020) This strain also carries a (T to A) missense mutation in pry-1 which results in a PRY-1 N354K amino acid substitution.] bar-1(ga80) hermaphrodites are usually Egl and often have a protruding vulva (Pvl), although approx. 40% of animals appear WT on plates. Also slightly Unc. In bar-1(ga80) hermaphrodites any of the six vulval precursor cells (P3.p - P8.p) can sometimes fuse with hyp7 without dividing, and P5.p - P7.p can adopt the tertiary cell fate instead of the primary or secondary fates. In addition, the neuroblast QL and its progeny migrate towards the anterior instead of the posterior, and the cell P12 usually adopts the fate of P11. bar-1(ga80) do mate, but poorly. bar-1 encodes a beta-catenin molecule and the ga80 mutation is predicted to cause an early truncation of the protein. |
| EW23 | unc-104(e1265) rol-6(e187) II. | C. elegans | Dpy. Roller. |
| EW32 | lin-26(ga91) II. | C. elegans | Pvl. Egl. |
| EW33 | unc-4(e120) pvl-4(ga96) II. | C. elegans | Egl. Pvl. Misshapen larvae (some lethal). Unc. |
| EW34 | egl-18(ga97) IV. | C. elegans | Egl. Rol. |
| EW35 | sem-4(ga82) I. | C. elegans | Egl. |
| EW45 | smg-1(e1228) I; unc-30(e191) IV; deIs1. | C. elegans | deIs1[unc-30(+) + lin-39TL::GFP (yeast DNA)]. A translational fusion of GFP to the C terminus of LIN-39. Present on a YAC. Yeast chromosomal DNA was injected and integrated. Expression is very faint. |
| EW49 | unc-30(e191) IV; deIs12. | C. elegans | deIs12[bar1p::GFP + pSC11(unc-30(+))]. Green worms that are non-Unc. |
| EW5 | pvl-6(ga81) V. | C. elegans | Egl. |
| EW51 | pvl-5(de4) II. | C. elegans | de4 is a weaker allele of pvl-5. Weakly penetrant Egl and Pvl phenotype. Low penetrance embryonic lethal and gonad migration defects. pvl-5(de4) animals have fewer Pn.p cells in the ventral midline at mid-L2 larval stage. |
| EW52 | lin-44(n1792) I; cwn-2(ok895) IV. | C. elegans | |
| EW53 | lin-44(n1792) I; cwn-1(ok546) II; cwn-2(ok895) IV. | C. elegans | |
| EW55 | dpy-20(e1362) IV; deEx100. | C. elegans | deEx100 [ajm-1::GFP + HinfI digested complex DNA + lin-44p::4xNLS::GFP + dpy20(+)]. Pick non-Dpy to maintain. |
| EW56 | dpy-20(e1362) IV; deEx101. | C. elegans | deEx101[dpy20(+); ajm-1::GFP; HinfI digested complex DNA; cwn-1p::4xNLSGFP]. Pick non-Dpy to maintain. |
| EW57 | dpy-20(e1362) IV; deEx102. | C. elegans | deEx102[dpy20(+); ajm-1::GFP; HinfI digested complex DNA; egl-20p::4xNLSGFP]. Pick non-Dpy to maintain. |
| EW58 | dpy-20(e1362) IV; deEx103. | C. elegans | deEx103[dpy20(+); ajm-1::GFP; HinfI digested complex DNA; cwn-2p::4xNLSGFP]. Pick non-Dpy to maintain. |
| EW59 | dpy-20(e1362) IV; deEx104. | C. elegans | deEx104 [ajm-1::GFP + mom-2p::4xNLS::GFP + dpy20(+) + HinfI digested complex DNA]. Pick non-Dpy to maintain. |
| EW61 | dpy-20(e1282) IV; him-5(e1490) V; deIs4. | C. elegans | deIs4 [ajm-1::GFP + GFP::lin-39(YAC) + dpy-20(+)]. A transcriptional fusion of GFP to the ATG of lin-39, with a stop codon between GFP and LIN-39 sequences. Present on a YAC. Yeast chromosomal DNA was injected and integrated. Expression is very faint and may get fainter over time. Freeze upon receipt. |
| EW62 | smg-1(e1228) I; dpy-20(e1282) IV; him-5(e1490) V; deIs4. | C. elegans | deIs4 [ajm-1::GFP + GFP::lin-39(YAC) + dpy-20(+)]. A transcriptional fusion of GFP to the ATG of lin-39, with a stop codon between GFP and LIN-39 sequences. Present on a YAC. Yeast chromosomal DNA was injected and integrated. Expression is very faint and may get fainter over time. Therefore, freeze upon receipt. |
| EW68 | lin-44(n1792) I; cwn-1(ok546) II. | C. elegans | |
| EW72 | cwn-1(ok546) II; cwn-2(ok895) IV. | C. elegans | |
| EW75 | cwn-2(ok895) IV/nT1 [qIs51] (IV;V); dpy-11(e1180) mom-2(or42) V/nT1 [qIs51] (IV;V). | C. elegans | Heterozygotes are WT and GFP+. Segregate more WT and GFP+, Dpys, and dead eggs. Dpys give only embryonic lethals. |
| EW77 | lin-44(n1792) I; egl-20(n585) IV/nT1 [qIs51] (IV;V); dpy-11(e1180) mom-2(or42) V/nT1 [qIs51] (IV;V). | C. elegans | |
| EW79 | cwn-1(ok546) II; egl-20(n585) IV/nT1 [qIs51] (IV;V); dpy-11(e1180) mom-2(or42) V/nT1 [qIs51] (IV;V). | C. elegans | Heterozygotes are WT and GFP+. Segregate more WT and GFP+, Dpys, and dead eggs. Dpys give only embryonic lethals. |
| KN594 | cwn-1(ok546) II; egl-20(n585) IV. | C. elegans | |
| KS46 | lin-44(n1792) I; egl-20(n585) IV. | C. elegans | |
| SD551 | let-60(ga89) IV. | C. elegans | Temperature sensitive. Nearly WT at 15C. At 20C the animals are 18% Muv and brood size is 88. At 25C the animals are 57% Muv and almost sterile (brood size is 6). Maintain at 15C. |
Alleles contributed by this laboratory
| Allele | Type | DNA Change | Protein Change |
|---|---|---|---|
| de4 | Allele |