Laboratory Information

NameDF View on WormBase
Allele designationny
HeadFitch, David
InstitutionNew York University, New York
Address New York University
Department of Biology
100 Washington Square East
New York 10003
United States
Website https://wormtails.bio.nyu.edu/Home.html
Gene classes lep 

Strains contributed by this laboratory

Strain Genotype Species Description
DF22 tlp-1(bx85) unc-24(e138) IV; him-5(e1490) V. C. elegans Unc. Although hermaphrodites appear WT in other ways, there are some problems with T cell lineages (affecting the phasmids) and tail cell fusions. Variably Dyf. Male tail tip morphogenesis is also defective, resulting in blobby, "leptoderan" tails. Males are infertile due to an inability to properly copulate.
DF5010 Metarhabditis blumi wild isolate. Metarhabditis blumi Metarhabditis blumi wild isolate. Formerly known as Rhabditis blumi. Isolated by J.P. Blum in April 1971 in a dung heap near Valencia, Spain. Gonochoristic. Grows well at 16-24C on OP50. Forms dauer larvae on overcrowded and starved plates. Freezes easily with C. elegans protocols with 80% viability.
DF5022 Pelodera strongyloides wild isolate. Pelodera strongyloides WT strain, Pelodera strongyloides. Isolated in April 1948 from pustules in the skin of a cow with dermatitis in central Illinois (see Levine et al 1950). Gonochoristic. May cause dermatitis in mammals (such as domesticated animals, and, rarely, humans). Grows well at 16-24C on OP50. Dauer larvae are the infective stage and are thermotactic. Freezes easily with C. elegans protocols with 70% viability. Previously called Pelodera strongyloides dermatitica by the CGC.
DF5081 Caenorhabditis japonica. C. japonica Male-female strain. Maintain by mating. Strain used for sequencing. DF5081 is the strain of Caenorhabditis japonica that Karin Kiontke generated with 20x incrossing of DF5080. http://genome.wustl.edu/pub/organism/Invertebrates/Caenorha bditis_japonica/assembly/Caenorhabditis_japonica-3.0.2/ASSE MBLY
DF5112 Caenorhabditis drosophilae wild isolate. C. drosophilae 20x inbred line derived from DF5077. Male-female strain. Heat shock 2.5-3.0 hrs at 30C immediately prior to freezing.
DF5152 Caenorhabditis sp. 30 wild isolate. C. sp. 30 Isofemale line. Isolated from a rotten lemon collected at Chan Chich Lodge in the town of Gallon Jug, Orange Walk, Belize (17.56399°N, 89.046282°W) on 11/15/12.
DF5173 Caenorhabditis sp. 8 wild isolate. C. sp. 8 Male-female strain. 20x inbred line derived from TMG13. Heat shock 2.5-3.0 hrs at 30C immediately prior to freezing.
DF64 nyDf1(ny15) IV; him-5(e1490) V. C. elegans Deficiency covers at least all of cosmid T23G4; right breakpoint may be in cosmid C47A4. Fails to complement tlp-1(bx85). Viable and fertile. Although hermaphrodites appear WT in other ways, there are some problems with T cell lineages (affecting the phasmids) and tail cell fusions. Variably Dyf. Male tail tip morphogenesis is also defective, resulting in blobby, "leptoderan" tails. Males are infertile due to an inability to properly copulate.
EM347 tlp-1(bx85) IV; him-5(e1490) V. C. elegans Although hermaphrodites appear WT in other ways, there are some problems with T cell lineages (affecting the phasmids) and tail cell fusions. Variably Dyf. Male tail tip morphogenesis is also defective, resulting in blobby, "leptoderan" tails. Males are infertile due to an inability to properly copulate. tlp-1 encodes a nuclear protein with a single C2H2-type zinc finger domain and an N-terminal "SPLALLA" domain, similar to that of Sp1 transcription factors of vertebrates. The bx85 mutation involves a truncation of TLP-1 due to a frameshift caused by a 5-bp deletion.
EM435 Oscheius myriophila wild isolate. Oscheius myriophila WT strain. Isolated by D. Fitch in June 1990 from soil in Scott Emmons' compost heap in the Fort Greene section of Brooklyn, NY. A second strain, DF5038, was isolated from the same location one year later from the head and ventral segments of a male pill bug (Armadillidium vulgare). Hermaphroditic. Males are easily isolated by heat shocking L4 or early adult hermaphrodites at 30C for 6-12 hrs. Grows well at 6-25C on OP50. Dauer larvae accumulate under starved or overcrowded conditions. Freezes easily using C. elegans protocols with 90% viability. Previously called Rhabditis sp. See also WBPaper00003418.
JU2156 Caenorhabditis zanzibari wild isolate. C. zanzibari Isolated by M.-A. Félix from rotting fruits of Calophyllum inophyllum (red mahogany) sampled in the Jozani National Forest, Zanzibar, Tanzania (6.2715°S, 39.416°E), on 3/9/2012. Previously known as Caenorhabditis sp. 26.
JU2161 Caenorhabditis zanzibari wild isolate. C. zanzibari Male-Female strain. Isolated by M.-A. Félix from a rotting mandarin sampled on a farm in the Dole district, Zanzibar, Tanzania (6.1074°S, 39.2515°E) , on 3/7/2012. Previously known as Caenorhabditis sp. 26.
NIC113 Caenorhabditis guadeloupensis wild isolate. C. guadeloupensis Caenorhabditis sp. 20 Isolated by Christian Braendle from rotten Heliconia flowers, Souffriere Forest trail Guadeloupe (16.0328, -61.676) in March 2010.
PS1010 Caenorhabditis angaria C. angaria Male-female strain. Gonochoristic. Grows well on OP50. Freezes well with C. elegans protocol. Isolated by Robin Giblin-Davis in Oct. 1990 in Dade County, FL in the abdomen of an adult female of Metamasius hemipterus. Consolt Walter Sudhaus or Robin Giblin-Davis for appropriate taxonomy before publication. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. sp. 3 in Kiontke and Sudhaus Wormbook Ecology chapter.
QG555 Caenorhabditis sp. 24 wild isolate. C. sp. 24 Isolated by Annalise Paaby from an orange peel collected beneath a fig tree on State Street, Santa Barbara, CA (34.421629, -119.702021) in July 2010.
QG702 Caenorhabditis panamensis wild isolate. C. panamensis Isofemale line. Isolated by M. Rockman from a rotting palm fruit (?) on the Snyder-Molino trail on Barro Colorado Island, Panama (9°09.656' N, 79°50.490'W) on 4/24/12. Previously known as Caenorhabditis sp. 28.
QG704 Caenorhabditis becei wild isolate. C. becei Isofemale line. Isolated by M. Rockman from a rotten Gustavia superba flower on Barro Colorado Island, Panama (9°09.222'N, 79°49.564'W) on 4/25/12. Previously known as Caenorhabditis sp. 29.
QX2263 Caenorhabditis sp. 27 wild isolate. C. sp. 27 Isolated from garden soil in Buenos Aires, Argentina on 3/2/2012.
ZF1092 Caenorhabditis sp. 25 wild isolate. C. sp. 25 Isolated by Adeline Seah and Takao Inoue from a rotten fruit in an urban garden in Singapore (1.32°N, 103.82°E) on 7/19/2006.
ZF1222 Caenorhabditis sp. 25 wild isolate. C. sp. 25 Isolated by Adeline Seah and Takao Inoue from a rotten fruit in an urban garden in Singapore (1.45°N, 103.72°E) on 7/19/2007.
This laboratory hasn't submitted any alleles to the CGC.