Laboratory Information
| Name | DE View on WormBase |
|---|---|
| Allele designation | dn |
| Head | James W. Dennis |
| Institution | Mount Sinai Hospital, Toronto, Ontario, Canada |
| Address | Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital Room 988 600 University Avenue Toronto M5G 1X5 Canada |
| Gene classes | cbd |
Strains contributed by this laboratory
| Strain | Genotype | Species | Description |
|---|---|---|---|
| DE112 | sup-46(qa710) I; dnSi4 II; unc-119(ed9) III. | C. elegans | dnSi4 [gna-1::GFP::gna-1 3’UTR + Cbr-unc-119(+)] inserted into ttTi5605 on LG II. Unknown if unc-119(ed9) III is homozygous or heterozygous in this strain. |
| DE115 | dnSi8 I; unc-119(ed3) III; dnIs22. | C. elegans | dnSi8 [tdp1::flag::mCherry + Cbr-unc119(+)] inserted into ttTi5605 on LG II. Nuclear-localized mCherry. dnIs22 [sup-46::GFP + unc-119(+)] (site of integration unknown). Strong nuclear-localized GFP expression. [NOTE: This strain was produced by crossing two parental strains carrying strong unc-119 loss of function alleles. One parent was carrying ed3; the allele in the other parental strain is unknown.] |
| DE116 | dnSi9II; unc-119(ed9) III. | C. elegans | dnSi9 [sup-46::GFP::sup-46 3’UTR + Cbr-unc-119(+)] inserted into ttTi5605 on LG II. Strong nuclear-localized GFP expression (expression from dnIs22 is brighter). |
| DE12 | sup-46(qa710) I. | C. elegans | Superficially WT. Suppressor of gna-2. Reduced brood counts at all temperatures (very strongly reduced at 26C). Exhibits mating-dependent progressive hermaphrodite sterility. Reduced embryo survival following heat shock. |
| DE130 | unc-119(e2488) III; dnIs24. | C. elegans | dnIs24 [sup46::flag::mCherry + Cbr-unc-119(+)]; site of integration unknown. Strong nuclear-localized mCherry. |
| DE83 | egg-1(ok1459) III. | C. elegans | Temperature sensitive embryonic lethal. Maintain at 15 C. 6% hatching rate at 25 C. Reference: Johnston et al. (2010) Curr Biol. |
| DE90 | oxIs318 II; unc-119(ed3 or e2498) ruIs32 III; ddIs6 V; dnIs17. | C. elegans | oxIs318 [spe-11p::mCherry::histone + unc-119(+)] II. ruIs32 [pie-1p::GFP::histone H2B + unc-119(+)] III. ddIs6 [tbg-1::GFP + unc-119(+)] V. dnIs17 [pie-1p::GFP::hPLCIII?PH domain + unc-119(+)]. Maintain under normal conditions. Pick GFP+ to maintain. Reference: Johnston et al. (2010) Curr Biol. |
| NW1287 | gly-1(ev686) II. | C. elegans | Phenotypically wild type. F44F4.6 |
| XA728 | gly-2(qa700) I. | C. elegans | Phenotypically WT. C55B7.2 |
| XA738 | gly-16(qa701) I. | C. elegans | Phenotypically wild type. T15D6.2 |
| XA749 | gly-1(qa702) II. | C. elegans | Phenotypically wild type. F44F4.6 |
| XA762 | gly-2(qa703) I. | C. elegans | Phenotypically WT. C55B7.2 |
| XA763 | gly-18(qa704) I. | C. elegans | Phenotypically wild type. F22D6.11 |
| XA774 | gld-1(q485)/gna-2(qa705) unc-55(e1170) I. | C. elegans | Heterozygotes are WT and segregate WT, steriles with a tumorous germline (gld-1 homozygotes), and Uncs that lay non-refractile eggs that fail to hatch (gna-2 unc-55) homozygotes. |
| XA780 | gna-2(qa705)/goa-1(n499) I. | C. elegans | Heterozygotes are Egl and Paralyzed. Segregate embryonic lethals (n499) homozygotes. Segregates WT animals that lay only non-refractile eggs that fail to hatch. Pick paralyzed Egl worms to maintain (eggs on the plate should all be pale brown and non-refractile; presence of refractile eggs indicates a recombination has occurred). XA780 recombines at a frequency of about 1%. |
| XA792 | sup-46(qa707) I. | C. elegans | Superficially WT. Suppressor of gna-2. Reduced brood counts at all temperatures (strongly reduced at 26C). Reduced embryo survival following heat shock. Hypomorph. |
| XA797 | sup-46(qa708) I. | C. elegans | Superficially WT. Suppressor of gna-2. Reduced brood counts at all temperatures (very strongly reduced at 26C). Exhibits mating-dependent progressive hermaphrodite sterility. Reduced embryo survival following heat shock. |
This laboratory hasn't submitted any alleles to the CGC.