Laboratory Information

NameBU View on WormBase
Allele designationkq
HeadLee Myeongwoo
InstitutionBaylor University, Waco, TX
Address 101 Bagby Ave
Department of Biology
Baylor University
waco 76706
United States
Website http://www.baylor.edu/biology/index.php?id=68801
Gene classes

Strains contributed by this laboratory

Strain Genotype Species Description
BU715 unc-112(kq715) V. C. elegans Viable, fertile. Partially-penetrant defective distal tip cell (DTC) migration. unc-112(kq715) is a L715E substitution. Reference: Park A, et al. (2020). A novel mutation in unc-112/kindlin locus causes distal tip cell migration defects. microPublication Biology. 10.17912/micropub.biology.000265.
BU7222 pat-3(st564) III; kqEx73. C. elegans kqEx73 [pat-3(sp) + rab-3::RFP + cki-1::GFP]. Pick RFP+ to maintain. kqEx73 carries a form of pat-3 gene with splicing defects; rescues pat-3 null allele. pat-3(sp) is a frameshift mutation in the splice acceptor region (ag to aa) that abolishes conserved interaction domains such as the NPxY motifs and creates a splice variant with an extra 19 amino acids. The pat-3(sp) animals not only produce mutant pat-3, but also express the regular splice form due to utilization of an unusual splice acceptor. Abnormal Distal Tip Cell migration and pat-3 gene splicing (intron 7) defects. Reference: Kihira S, et al. PLoS One. 2012;7(8):e42425.
BU745 unc-52(kq745) II. C. elegans Superficially wild-type. Reduced motility in thrashing assays. unc-52(kq745) removes the RGD sequence (amino acids 746, 747, and 748). Reference: Wilsey R, et al. (2020). Two alleles of unc-52 locus disrupting potential cell-binding motif of UNC-52. microPublication Biology. 10.17912/micropub.biology.000250.
BU748 unc-52(kq748) II. C. elegans Superficially wild type. Reduced motility in thrashing assay. unc-52(kq748) disrupts the RGD sequence (D748E substitution). Reference: Wilsey R, et al. (2020). Two alleles of unc-52 locus disrupting potential cell-binding motif of UNC-52. microPublication Biology. 10.17912/micropub.biology.000250.
JE32 pat-3(st564) III; mwEx32. C. elegans mwEx32 [pat-3(YYFF) + sur-5::GFP]. Pick GFP+ to maintain. mwEx32 carries a mutant form of pat-3 gene with tyrosine (Y) to phenylalanine (F) mutations; rescues pat-3 null allele. Abnormal DTC migration. Reference: Lee M, et al. J Biol Chem. 2001 Sep 28;276(39):36404-10.
JE443 pat-3(st564) III; mwEx443. C. elegans mwEx443 [pat-3(+) + sur-5::GFP]. Pick GFP+ to maintain. mwEx443 contains wild type contains pat-3 genomic DNA, rescues pat-3 null allele. Reference: Lee M, et al. J Biol Chem. 2001 Sep 28;276(39):36404-10.
This laboratory hasn't submitted any alleles to the CGC.