Caenorhabditis Genetics Center (CGC)

Laboratory Information

NameAMP View on WormBase
Allele designationohm
HeadNicholas Stroustrup
InstitutionCentre de Regulacio Genomica, Barcelona, Spain
Address PRBB Piso 6
C/ Dr. Aiguader, 88
Barcelona 08003
Spain
Website http://www.crg.eu/en/programmes-groups/dynamics-living-systems
Gene classes

Strains contributed by this laboratory

Strain Genotype Species Description
AMP100 ieSi57 II; rpb-2(cer135[rpb-2::GFP(delta)piRNA::AID*::3xFLAG]) III. C. elegans ieSi57 [eft-3p::TIR1::mRuby::unc-54 3'UTR + Cbr-unc-119(+)] II. cer135 is a rpb-2::GFP(delta)piRNA::AID*::3xFLAG tag inserted into the endogenous rpb-2 locus. This strain allows auxin-dependent disruption of RNA polymerase II with dose-dependent lifespan shortening. Reference: Oswal N, et al. PLoS Comput Biol. 2022 Sep 30;18(9):e1010415. PMID: 36178967.
AMP101 weSi174 II; daf-2(syb1177[daf-2::AID*::TEV::3xFLAG]) III. C. elegans weSi174 [eif-3.Bp::TIR1::linker::mCherry(dpiRNA)::tbb-2 3'UTR + unc-119(+)] II. Auxin-inducible degradation of DAF-2::AID* causes dauer formation. Reference: Eder M, et al. Cell. 2024 Jul 25;187(15):3919-3935.e19. doi: 10.1016/j.cell.2024.05.050. PMID: 38908368.
AMP116 weSi174 II; daf-2(syb1177[daf-2::AID*::TEV::3xFLAG]) glp-1(e2141) III. C. elegans weSi174 [eif-3.Bp::TIR1::linker::mCherry(dpiRNA)::tbb-2 3'UTR + unc-119(+)] II. Maintain at 20C or less. Sterile at 25C. Auxin-inducible degradation of DAF-2::AID* causes dauer formation. Reference: Eder M, et al. Cell. 2024 Jul 25;187(15):3919-3935.e19. doi: 10.1016/j.cell.2024.05.050. PMID: 38908368.
AMP158 ohmSi10 II. C. elegans ohmSi10 [eft-3p::TIR1(F79A)::mRuby::unc-54 3'UTR + Cbr-unc-119(+)] II. Single copy transgene inserted into chromosome II (oxTi179). Pan-somatic expression of modified Arabidopsis thaliana TIR1(F79A) tagged with mRuby for super-sensitive auxin-inducible degradation in somatic tissues using 5-Ad-IAA as a ligand. Generated by CRISPR/Cas9 modification of ieSi57 in parental strain CA1200. ohmSi10 previously referred to as ohm10. Reference: Vicencio J, et al. Nat Commun. 2025 Dec 12;16(1):10848. doi: 10.1038/s41467-025-66347-x. PMID: 41388022.
AMP175 ohmSi24 Iv. C. elegans ohmSi24 [unc-119p::TIR1(F79G)::mRuby] (IV: -0.05). Neuron-specific expression of modified Arabidopsis thaliana TIR1(F79G) tagged with mRuby for auxin-inducible degradation (AIDv2) in neuronal tissues. Generated by CRISPR/Cas9 modification of emcSi70 in parental strain HAL227. ohmSi24 previously referred to as ohm24. Reference: Vicencio J, et al. Nat Commun. 2025 Dec 12;16(1):10848. doi: 10.1038/s41467-025-66347-x. PMID: 41388022.
AMP184 ohmSi8 II. C. elegans ohmSi8 [eft-3p::TIR1(F79G)::mRuby::unc-54 3'UTR + Cbr-unc-119(+)] II. Single copy transgene inserted into chromosome II (oxTi179). Pan-somatic expression of modified Arabidopsis thaliana TIR1(F79G) tagged with mRuby for auxin-inducible degradation (AIDv2) in somatic tissues. Generated by CRISPR/Cas9 modification of ieSi57 in parental strain CA1200. ohmSi8 previously referred to as ohm8. Reference: Vicencio J, et al. Nat Commun. 2025 Dec 12;16(1):10848. doi: 10.1038/s41467-025-66347-x. PMID: 41388022.
AMP245 ohmSi49 IV. C. elegans ohmSi49 [eft-3p::TIR1::SL2::NLS::mTagBFP2::tbb-2 3'UTR] IV. Pan-somatic expression of TIR1 transgene with nuclear-localized mTagBFP2 co-expression marker. ohmSi49 previously referred to as ohm49. Reference: Vicencio J, et al. Nat Commun. 2025 Dec 12;16(1):10848. doi: 10.1038/s41467-025-66347-x. PMID: 41388022.
AMP267 ohmSi53. C. elegans ohmSi53 [eft-3p::TIR1::SL2::NLS::mTagBFP2::eft-3 3'UTR] IV. Pan-somatic expression of a TIR1 transgene with nuclear-localized mTagBFP2 co-expression marker. Generated by CRISPR/Cas9 modification of ohmSi49 in parental strain AMP245. ohmSi53 previously referred to as ohm53. Reference: Vicencio J, et al. Nat Commun. 2025 Dec 12;16(1):10848. doi: 10.1038/s41467-025-66347-x. PMID: 41388022.l. Nature Communications (2025).
This laboratory hasn't submitted any alleles to the CGC.