Laboratory Information
| Name | AMH View on WormBase |
|---|---|
| Allele designation | sos |
| Head | Andrea Holgado |
| Institution | St Edward's University, Austin, TX |
| Address | St Edward's University 3001 S. Congress Ave Austin 78704 United States |
| Gene classes |
Strains contributed by this laboratory
| Strain | Genotype | Species | Description |
|---|---|---|---|
| AMH1 | unc-33(e204) IV; sosIs5. | C. elegans | sosIs5 [rab-3p::Cerulean-Venus::lgg-1 + unc-119(+)]. Paralyzed Unc. |
| AMH11 | wpIs36 I; sosIs5. | C. elegans | wpIs36 [unc-47p::mCherry] I. sosIs5 [rab-3p::Cerulean-Venus::lgg-1 + unc-119(+)]. |
| AMH13 | wpIs36 I; unc-33(e204) IV; sosIs5. | C. elegans | wpIs36 [unc-47p::mCherry] I. sosIs5 [rab-3p::Cerulean-Venus::lgg-1 + unc-119(+)]. Paralyzed Unc. |
| AMH151 | juIs76 II; daf-7(e1372) III. | C. elegans | juIs76 [unc-25p::GFP + lin-15(+)] II. Maintain at 15C. Temperature sensitive dauer constitutive. 100% dauers at 25C. Leaky at 20C. Crowds. Growth slow. GFP expression in GABAergic motor neurons. |
| AMH23 | wpIs36 I; unc-33(e1193) IV; sosIs5. | C. elegans | wpIs36 [unc-47p::mCherry] I. sosIs5 [rab-3p::Cerulean-Venus::lgg-1 + unc-119(+)]. Unc; almost paralyzed. Growth slow. |
| AMH25 | dhc-1(or352) I; sosIs5. | C. elegans | sosIs5 [rab-3p::Cerulean-Venus::lgg-1 + unc-119(+)]. Maintain at 15 degrees. Temperature-sensitive embryonic lethal. 100% dead embryos at 25 degrees. Small, posteriorly displaced first mitotic spindle with defective chomosome segregation and cytokinesis. |
| AMH26 | unc-104(e1265) II; sosIs5. | C. elegans | sosIs5 [rab-3p::Cerulean-Venus::lgg-1 + unc-119(+)]. Unc. Slow moving. |
| AMH3 | unc-33(mn407) IV; sosIs5. | C. elegans | sosIs5 [rab-3p::Cerulean-Venus::lgg-1 + unc-119(+)]. Unc. |
| AMH30 | juIs76 II; daf-2(e1370) III. | C. elegans | juIs76 [unc-25p::GFP + lin-15(+)] II. Maintain at 15C. Temperature-sensitive dauer constitutive. |
| AMH31 | juIs76 II; unc-33(e1193) IV. | C. elegans | juIs76 [unc-25p::GFP + lin-15(+)] II. Unc; almost paralyzed. Growth slow. |
| AMH32 | juIs76 II; unc-33(mn407) IV. | C. elegans | juIs76 [unc-25p::GFP + lin-15(+)] II. |
| AMH34 | juIs76 II; unc-33(e204) IV. | C. elegans | juIs76 [unc-25p::GFP + lin-15(+)] II. |
| AMH36 | juIs76 II; daf-2(e1370) III; unc-33(mn407) IV. | C. elegans | juIs76 [unc-25p::GFP + lin-15(+)] II. Unc. Maintain at 15C. Synthetic Lethality at 25C. |
| AMH38 | juIs76 II; daf-2(e1370) III; unc-33(e204) IV. | C. elegans | juIs76 [unc-25p::GFP + lin-15(+)] II. |
| AMH43 | unc-33(e204) IV; adIs2122. | C. elegans | adIs2122 [lgg-1p::GFP::lgg-1 + rol-6(su1006)]. Rollers. Unc. |
| AMH46 | juIs76 II; daf-2(e1370) III; unc-33(e1193) IV. | C. elegans | Maintain at 15C. Synthetic Lethality at 25C. Unc; almost paralyzed. Growth slow. |
| AMH5 | unc-119(ed3) III; sosIs5. | C. elegans | sosIs5 [rab-3p::Cerulean-Venus::lgg-1 + unc-119(+)]. |
| AMH50 | juIs76; bec-1(ok691) IV/nT1 [qIs51] (IV;V). | C. elegans | juIs76 [unc-25p::GFP + lin-15(+)] II. Heterozygotes are wild-type with pharyngeal GFP signal, and segregate WT GFP, arrested nT1[qIs51] aneuploids, and non-pharyngeal GFP bec-1 homozygotes. Homozygous nT1[qIs51] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. |
| AMH55 | daf-2(e1370) III; otIs117 IV. | C. elegans | otIs117 [unc-33p::GFP + unc-4(+)] IV. Maintain at 15C. Temperature-sensitive dauer constitutive. Pan-neuronal GFP. |
| AMH57 | unc-33(mn407); olaEx3013. | C. elegans | olaEx3013 [ttx-3p:mCherry::eGFP::lgg-1 + unc-122p::mCherry]. Pick animals with mCherry+ coelomocytes to maintain array. Unc. Tandem tags on LGG-1 label immature autophagosomes with both GFP and mCherry, but because GFP is preferentially quenched in an acidic environment, mature structures lose their GFP signal and display solely mCherry signal. Reference: Hill SE & Colon-Ramos D. 2018 bioRxiv 287144; doi: https://doi.org/10.1101/287144 |
| AMH59 | unc-33(e204); olaEx3013. | C. elegans | olaEx3013 [ttx-3p:mCherry::eGFP::lgg-1 + unc-122p::mCherry]. Pick animals with mCherry+ coelomocytes to maintain array. Paralyzed Unc. Tandem tags on LGG-1 label immature autophagosomes with both GFP and mCherry, but because GFP is preferentially quenched in an acidic environment, mature structures lose their GFP signal and display solely mCherry signal. Reference: Hill SE & Colon-Ramos D. 2018 bioRxiv 287144; doi: https://doi.org/10.1101/287144 |
| AMH61 | unc-13(e51) I; ddi-1(ok1468) IV. | C. elegans | ddi-1 also known as vsm-1. |
| AMH67 | unc-33(e204) IV; daf-2(e1370) III. | C. elegans | Maintain at 15C. Synthetic Lethality at 25C. |
| AMH69 | unc-33(mn407) IV; daf-2(e1370) III. | C. elegans | Maintain at 15C. Synthetic Lethality at 25C. |
| AMH7 | unc-51(e369) V; sosIs5. | C. elegans | sosIs5 [rab-3p::Cerulean-Venus::lgg-1 + unc-119(+)]. Paralyzed Unc. Slow growth. |
| AMH71 | unc-33(e1193) IV; daf-2(e1370) III. | C. elegans | Maintain at 15C. Synthetic Lethality at 25C. |
| AMH73 | ddi-1(ok1468) IV; sosIs6. | C. elegans | sosIs6 [C01G5.6p::C01G5.6::GFP]. ddi-1 also known as vsm-1. |
| AMH82 | ccIs4251 I; ddi-1(ok1468) IV. | C. elegans | ccIs4251 [myo-3p::GFP::LacZ::NLS + myo-3p::mitochondrial GFP + dpy-20(+)] I. ddi-1 also known as vsm-1. |
| AMH91 | unc-104(e1265) II; olaEx3013. | C. elegans | olaEx3013 [ttx-3p::mCherry::eGFP::lgg-1 + unc-122p::mCherry]. Pick animals with mCherry+ coelomocytes to maintain array. Unc. Slow moving. Tandem tags on LGG-1 label immature autophagosomes with both GFP and mCherry, but because GFP is preferentially quenched in an acidic environment, mature structures lose their GFP signal and display solely mCherry signal. Reference: Hill SE & Colon-Ramos D. 2018 bioRxiv 287144; doi: https://doi.org/10.1101/287144 |
| AMH96 | sosIs3. | C. elegans | sosIs3 [unc-119p::ddi-1::GFP]. ddi-1 also known as vsm-1. |
This laboratory hasn't submitted any alleles to the CGC.