Laboratory Information

NameAMH View on WormBase
Allele designationsos
HeadAndrea Holgado
InstitutionSt Edward's University, Austin, TX
Address St Edward's University
3001 S. Congress Ave
Austin 78704
United States
Gene classes

Strains contributed by this laboratory

Strain Genotype Species Description
AMH1 unc-33(e204) IV; sosIs5. C. elegans sosIs5 [rab-3p::Cerulean-Venus::lgg-1 + unc-119(+)]. Paralyzed Unc.
AMH11 wpIs36 I; sosIs5. C. elegans wpIs36 [unc-47p::mCherry] I. sosIs5 [rab-3p::Cerulean-Venus::lgg-1 + unc-119(+)].
AMH13 wpIs36 I; unc-33(e204) IV; sosIs5. C. elegans wpIs36 [unc-47p::mCherry] I. sosIs5 [rab-3p::Cerulean-Venus::lgg-1 + unc-119(+)]. Paralyzed Unc.
AMH151 juIs76 II; daf-7(e1372) III. C. elegans juIs76 [unc-25p::GFP + lin-15(+)] II. Maintain at 15C. Temperature sensitive dauer constitutive. 100% dauers at 25C. Leaky at 20C. Crowds. Growth slow. GFP expression in GABAergic motor neurons.
AMH19 unc-33(e1193) IV; sosIs5. C. elegans sosIs5 [rab-3p::Cerulean-Venus::lgg-1 + unc-119(+)]. Unc; almost paralyzed. Growth slow.
AMH23 wpIs36 I; unc-33(e1193) IV; sosIs5. C. elegans wpIs36 [unc-47p::mCherry] I. sosIs5 [rab-3p::Cerulean-Venus::lgg-1 + unc-119(+)]. Unc; almost paralyzed. Growth slow.
AMH25 dhc-1(or352) I; sosIs5. C. elegans sosIs5 [rab-3p::Cerulean-Venus::lgg-1 + unc-119(+)]. Maintain at 15 degrees. Temperature-sensitive embryonic lethal. 100% dead embryos at 25 degrees. Small, posteriorly displaced first mitotic spindle with defective chomosome segregation and cytokinesis.
AMH26 unc-104(e1265) II; sosIs5. C. elegans sosIs5 [rab-3p::Cerulean-Venus::lgg-1 + unc-119(+)]. Unc. Slow moving.
AMH3 unc-33(mn407) IV; sosIs5. C. elegans sosIs5 [rab-3p::Cerulean-Venus::lgg-1 + unc-119(+)]. Unc.
AMH30 juIs76 II; daf-2(e1370) III. C. elegans juIs76 [unc-25p::GFP + lin-15(+)] II. Maintain at 15C. Temperature-sensitive dauer constitutive.
AMH31 juIs76 II; unc-33(e1193) IV. C. elegans juIs76 [unc-25p::GFP + lin-15(+)] II. Unc; almost paralyzed. Growth slow.
AMH32 juIs76 II; unc-33(mn407) IV. C. elegans juIs76 [unc-25p::GFP + lin-15(+)] II.
AMH34 juIs76 II; unc-33(e204) IV. C. elegans juIs76 [unc-25p::GFP + lin-15(+)] II.
AMH36 juIs76 II; daf-2(e1370) III; unc-33(mn407) IV. C. elegans juIs76 [unc-25p::GFP + lin-15(+)] II. Unc. Maintain at 15C. Synthetic Lethality at 25C.
AMH38 juIs76 II; daf-2(e1370) III; unc-33(e204) IV. C. elegans juIs76 [unc-25p::GFP + lin-15(+)] II.
AMH43 unc-33(e204) IV; adIs2122. C. elegans adIs2122 [lgg-1p::GFP::lgg-1 + rol-6(su1006)]. Rollers. Unc.
AMH46 juIs76 II; daf-2(e1370) III; unc-33(e1193) IV. C. elegans Maintain at 15C. Synthetic Lethality at 25C. Unc; almost paralyzed. Growth slow.
AMH5 unc-119(ed3) III; sosIs5. C. elegans sosIs5 [rab-3p::Cerulean-Venus::lgg-1 + unc-119(+)].
AMH50 juIs76; bec-1(ok691) IV/nT1 [qIs51] (IV;V). C. elegans juIs76 [unc-25p::GFP + lin-15(+)] II.  Heterozygotes are wild-type with pharyngeal GFP signal, and segregate WT GFP, arrested nT1[qIs51] aneuploids, and non-pharyngeal GFP bec-1 homozygotes. Homozygous nT1[qIs51] inviable. Pick WT GFP and check for correct segregation of progeny to maintain.
AMH53 dvIs50 I; sosIs5. C. elegans dvIs50 [snb-1::Abeta 1-42::3' UTR(long) + mtl-2::GFP] I. sosIs5 [rab-3p::Cerulean-Venus::lgg-1 + unc-119(+)].
AMH55 daf-2(e1370) III; otIs117 IV. C. elegans otIs117 [unc-33p::GFP + unc-4(+)] IV. Maintain at 15C. Temperature-sensitive dauer constitutive. Pan-neuronal GFP.
AMH57 unc-33(mn407); olaEx3013. C. elegans olaEx3013 [ttx-3p:mCherry::eGFP::lgg-1 + unc-122p::mCherry]. Pick animals with mCherry+ coelomocytes to maintain array. Unc. Tandem tags on LGG-1 label immature autophagosomes with both GFP and mCherry, but because GFP is preferentially quenched in an acidic environment, mature structures lose their GFP signal and display solely mCherry signal. Reference: Hill SE & Colon-Ramos D. 2018 bioRxiv 287144; doi: https://doi.org/10.1101/287144
AMH59 unc-33(e204); olaEx3013. C. elegans olaEx3013 [ttx-3p:mCherry::eGFP::lgg-1 + unc-122p::mCherry]. Pick animals with mCherry+ coelomocytes to maintain array. Paralyzed Unc. Tandem tags on LGG-1 label immature autophagosomes with both GFP and mCherry, but because GFP is preferentially quenched in an acidic environment, mature structures lose their GFP signal and display solely mCherry signal. Reference: Hill SE & Colon-Ramos D. 2018 bioRxiv 287144; doi: https://doi.org/10.1101/287144
AMH61 unc-13(e51) I; ddi-1(ok1468) IV. C. elegans ddi-1 also known as vsm-1.
AMH67 unc-33(e204) IV; daf-2(e1370) III. C. elegans Maintain at 15C. Synthetic Lethality at 25C.
AMH69 unc-33(mn407) IV; daf-2(e1370) III. C. elegans Maintain at 15C. Synthetic Lethality at 25C.
AMH7 unc-51(e369) V; sosIs5. C. elegans sosIs5 [rab-3p::Cerulean-Venus::lgg-1 + unc-119(+)]. Paralyzed Unc. Slow growth.
AMH71 unc-33(e1193) IV; daf-2(e1370) III. C. elegans Maintain at 15C. Synthetic Lethality at 25C.
AMH73 ddi-1(ok1468) IV; sosIs6. C. elegans sosIs6 [C01G5.6p::C01G5.6::GFP]. ddi-1 also known as vsm-1.
AMH82 ccIs4251 I; ddi-1(ok1468) IV. C. elegans ccIs4251 [myo-3p::GFP::LacZ::NLS + myo-3p::mitochondrial GFP + dpy-20(+)] I. ddi-1 also known as vsm-1.
AMH9 unc-51(e1189) V; sosIs5. C. elegans sosIs5 [rab-3p::Cerulean-Venus::lgg-1 + unc-119(+)]. Paralysed, Dpy, tends to curl. Egl.
AMH91 unc-104(e1265) II; olaEx3013. C. elegans olaEx3013 [ttx-3p::mCherry::eGFP::lgg-1 + unc-122p::mCherry]. Pick animals with mCherry+ coelomocytes to maintain array. Unc. Slow moving. Tandem tags on LGG-1 label immature autophagosomes with both GFP and mCherry, but because GFP is preferentially quenched in an acidic environment, mature structures lose their GFP signal and display solely mCherry signal. Reference: Hill SE & Colon-Ramos D. 2018 bioRxiv 287144; doi: https://doi.org/10.1101/287144
AMH96 sosIs3. C. elegans sosIs3 [unc-119p::ddi-1::GFP]. ddi-1 also known as vsm-1.
This laboratory hasn't submitted any alleles to the CGC.